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The annotation and conditions in this rule are derived from the following entries: A2T3S5 (VP3_ROTSH), Q6WAT6 (VP3_ROTBU)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_04128
    • taxon = Rotavirus
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 718 - 718 aligns to "H" in entry A2T3S5 (individually applies "For 2'-5'-phosphodiesterase activity")
    • Subsequence at position 556 - 692 aligns to entry A2T3S5 (individually applies "GTase/RTPase activity")
    • Subsequence at position 720 - 720 aligns to "T" in entry A2T3S5 (individually applies "For 2'-5'-phosphodiesterase activity")
    • Subsequence at position 693 - @CTER@ aligns to entry A2T3S5 (individually applies "2'-5'-phosphodiesterase activity")
    • Subsequence at position 429 - 555 aligns to entry A2T3S5 (individually applies "N7-methyltransferase activity")
    • Subsequence at position 797 - 797 aligns to "H" in entry A2T3S5 (individually applies "For 2'-5'-phosphodiesterase activity")
    • Subsequence at position 246 - 428 aligns to entry A2T3S5 (individually applies "2'-O-methyltransferase activity")
    • Subsequence at position 171 - 245 aligns to entry A2T3S5 (individually applies "N7-methyltransferase activity")
    • Subsequence at position 799 - 799 aligns to "T" in entry A2T3S5 (individually applies "For 2'-5'-phosphodiesterase activity")

... then these annotations are applied i

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namei

  • Recommended name:
    Protein VP3

Cleaved chain(s) or included domain(s)i

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> describes the function(s) of a protein.<p><a href='/help/function' target='_top'>More...</a></p>Functioni

  • Counteracts the host innate immune response thanks to its phosphodiesterase that degrades the 5'-triphosphorylated, 2'-5' linked adenylate oligomers produced by the host cell IFN-inducible 2',5'-oligoadenylate synthetase (OAS). The host RNaseL is therefore not activated.
  • Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms a VP1-VP3 complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores do not.

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

  • Contains a bipartite N7-methytransferase domain, a 2'-O-methytransferase domain and a GTase/RTPase domain. The C-terminus contains a phosphodiesterase domain that degrades the 5'-triphosphorylated, 2'-5' linked adenylate oligomers produced by the host cell in response to IFN stimulation.

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

  • Interacts with VP1. Interacts with VP2.

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    • Virion
    • Note: Attached inside the inner capsid as a minor component. There are about 11 to 12 copies per virion.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei

  • For 2'-5'-phosphodiesterase activity (to residues corresponding to position 718)
  • For 2'-5'-phosphodiesterase activity (to residues corresponding to position 720)
  • For 2'-5'-phosphodiesterase activity (to residues corresponding to position 797)
  • For 2'-5'-phosphodiesterase activity (to residues corresponding to position 799)

<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni

  • GTase/RTPase activity (to residues corresponding to positions 556 - 692)
  • 2'-5'-phosphodiesterase activity (to residues corresponding to positions 693 - @CTER@i)
  • N7-methyltransferase activity (to residues corresponding to positions 429 - 555)
  • 2'-O-methyltransferase activity (to residues corresponding to positions 246 - 428)
  • N7-methyltransferase activity (to residues corresponding to positions 171 - 245)

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO (Gene Ontology) termsi

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