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The annotation and conditions in this rule are derived from the following entries: Q8LEE8 (LISC_ARATH), P32875 (LIPA_YEAST)

If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 127 - 127 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 132 - 132 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 138 - 138 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 1 (4Fe-4S)")
    • Subsequence at position 158 - 158 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 162 - 162 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")
    • Subsequence at position 165 - 165 aligns to "C" in entry Q8LEE8 (individually applies "Iron-sulfur 2 (4Fe-4S-S-AdoMet)")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Lipoyl synthase, chloroplastic (EC:2.8.1.8)
    Alternative name(s):
    Lipoate synthase
    Short name:
    LS
    Short name:
    Lip-syn
    Lipoate synthase, plastidial
    Short name:
    LIP1p
    Lipoic acid synthase

Gene namei

  • Name:LIP1P

Sequence similaritiesi

Functioni

  • Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.

Catalytic activityi

  • Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.

Subcellular locationi

Pathwayi

  • Pathwayi: protein lipoylation via endogenous pathway

    This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]. This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Cofactori

  • [4Fe-4S] clusterNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Metal bindingi

  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 127)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 132)
  • Iron-sulfur 1 (4Fe-4S) (to residues corresponding to position 138)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 158)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 162)
  • Iron-sulfur 2 (4Fe-4S-S-AdoMet) (to residues corresponding to position 165)

Keywordsi

GO (Gene Ontology) termsi

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