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The annotation and conditions in this rule are derived from the following entries: Q97W94 (MTAP_SULSO), Q8U4Q8 (MTAP_PYRFU), Q8U2I1 (PNPH_PYRFU), Q9HZK1 (MTIP_PSEAE)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_01963
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented
    • Matches HAMAP signature MF_01963
    • taxon = Archaea
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 aligns to entry Q8U2I1
    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 aligns to entry Q8U2I1
    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 does not align to entry Q8U2I1
    • Subsequence at position 214 - 216 does not align to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 does not align to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 does not align to entry Q8U2I1
    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 aligns to entry Q8U2I1
    • Subsequence at position 214 - 216 does not align to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 aligns to entry Q8U2I1
    • Subsequence at position 169 - 169 does not align to "[SE]" in entry Q8U2I1
    • Subsequence at position 214 - 216 does not align to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 214 - 216 does not align to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 169 - 169 does not align to "[SE]" in entry Q8U2I1
    • Subsequence at position 169 - 169 does not align to "[SE]" in entry Q8U2I1
    • Subsequence at position 214 - 216 does not align to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 223 - 223 does not align to entry Q8U2I1
    • Subsequence at position 169 - 169 does not align to "[SE]" in entry Q8U2I1
    • Subsequence at position 223 - 223 does not align to entry Q8U2I1
    • Subsequence at position 223 - 223 aligns to entry Q8U2I1
    • Subsequence at position 214 - 216 aligns to "[DN]-x-[DA]" in entry Q97W94
    • Subsequence at position 16 - 16 aligns to "[ST]" in entry Q97W94 (individually applies "Phosphate")
    • Subsequence at position 58 - 59 aligns to "R-[HN]" in entry Q97W94 (individually applies "Phosphate binding")
    • Subsequence at position 91 - 92 aligns to "[ST]-A" in entry Q97W94 (individually applies "Phosphate binding")
    • Subsequence at position 190 - 190 aligns to "M" in entry Q97W94 (individually applies "Substrate; via amide nitrogen")
    • Subsequence at position 191 - 191 aligns to "[ST]" in entry Q97W94 (individually applies "Phosphate")
    • Subsequence at position 169 - 169 aligns to "[SE]" in entry Q8U2I1

... then these annotations are applied i

Protein namesi

  • Recommended name:
    S-methyl-5'-thioadenosine phosphorylase (EC:2.4.2.28)
    Alternative name(s):
    5'-methylthioadenosine phosphorylase
    Short name:
    MTA phosphorylase
    Short name:
    MTAP
  • Recommended name:
    Probable 6-oxopurine nucleoside phosphorylase (EC:2.4.2.1)
    Alternative name(s):
    Purine nucleoside phosphorylase
    Short name:
    PNP
  • Recommended name:
    Probable S-methyl-5'-thioinosine phosphorylase (EC:2.4.2.44)
    Alternative name(s):
    5'-methylthioinosine phosphorylase
    Short name:
    MTI phosphorylase
    Short name:
    MTIP
  • Recommended name:
    Purine nucleoside phosphorylase (EC:2.4.2.1)
    Short name:
    PNP

Gene namei

  • Name:mtnP

Subunit structurei

  • Homohexamer. Dimer of a homotrimer.
  • Homohexamer. Dimer of a homotrimer.
  • Homotrimer.
  • Homohexamer. Dimer of a homotrimer.

Catalytic activityi

  • S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.
  • Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
  • S-methyl-5'-thioinosine + phosphate = hypoxanthine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.
  • Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

Pathwayi

  • Pathwayi: L-methionine biosynthesis via salvage pathway

    This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route). This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.
  • Pathwayi: purine nucleoside salvage

    This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
    View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Functioni

  • Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
  • Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage.
  • Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.
  • Purine nucleoside phosphorylase involved in purine salvage.

Sequence similaritiesi

Miscellaneousi

  • Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.
  • Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.
  • Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.

Binding sitei

  • Phosphate (to residues corresponding to position 16)
  • Substrate; via amide nitrogen (to residues corresponding to position 190)
  • Phosphate (to residues corresponding to position 191)

Sitei

  • Important for substrate specificity (to residues corresponding to position 223)
  • Important for substrate specificity (to residues corresponding to position 169)

Regioni

  • Substrate binding (to residues corresponding to positions 214 - 216)
  • Phosphate binding (to residues corresponding to positions 58 - 59)
  • Phosphate binding (to residues corresponding to positions 91 - 92)

Keywordsi

GO (Gene Ontology) termsi

  • GO:0017061 S-methyl-5-thioadenosine phosphorylase activity
  • GO:0019509 L-methionine salvage from methylthioadenosine
  • GO:0006166 purine ribonucleoside salvage
  • GO:0016763 transferase activity, transferring pentosyl groups

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