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The annotation and conditions in this rule are derived from the following entries: P42450 (MASZ_CORGL), P37330 (MASZ_ECOLI), P9WK17 (MASZ_MYCTU)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00641
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 118 - 118 aligns to "V" in entry P37330 (individually applies "Acetyl-CoA; via carbonyl oxygen")
    • Subsequence at position 125 - 126 aligns to entry P37330 (individually applies "Acetyl-CoA binding")
    • Subsequence at position 274 - 274 aligns to "S" in entry P37330 (individually applies "Acetyl-CoA")
    • Subsequence at position 311 - 311 aligns to "R" in entry P37330 (individually applies "Acetyl-CoA")
    • Subsequence at position 338 - 338 aligns to "R" in entry P37330 (individually applies "Proton acceptor")
    • Subsequence at position 338 - 338 aligns to "R" in entry P37330 (individually applies "Glyoxylate")
    • Subsequence at position 427 - 427 aligns to "E" in entry P37330 (individually applies "Glyoxylate")
    • Subsequence at position 427 - 427 aligns to "E" in entry P37330 (individually applies "Magnesium")
    • Subsequence at position 452 - 455 aligns to entry P37330 (individually applies "Glyoxylate binding")
    • Subsequence at position 455 - 455 aligns to "D" in entry P37330 (individually applies "Magnesium")
    • Subsequence at position 536 - 536 aligns to "P" in entry P37330 (individually applies "Acetyl-CoA; via carbonyl oxygen")
    • Subsequence at position 617 - 617 aligns to "C" in entry P37330 (individually applies "Cysteine sulfenic acid (-SOH)")
    • Subsequence at position 631 - 631 aligns to "D" in entry P37330 (individually applies "Proton donor")
    • Subsequence at position 688 - 688 aligns to "C" in entry P37330 (individually applies "Cysteine sulfenic acid (-SOH)")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Malate synthase G (EC:2.3.3.9)

Gene namei

  • Name:glcB

Catalytic activityi

  • Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.

Subunit structurei

  • Monomer.

Pathwayi

  • Pathwayi: glyoxylate cycle

    This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate. This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sequence similaritiesi

Cofactori

Functioni

  • Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.

Subcellular locationi

Metal bindingi

  • Magnesium (to residues corresponding to position 427)
  • Magnesium (to residues corresponding to position 455)

Active sitei

  • Proton acceptor (to residues corresponding to position 338)
  • Proton donor (to residues corresponding to position 631)

Binding sitei

  • Acetyl-CoA; via carbonyl oxygen (to residues corresponding to position 118)
  • Acetyl-CoA (to residues corresponding to position 274)
  • Acetyl-CoA (to residues corresponding to position 311)
  • Glyoxylate (to residues corresponding to position 338)
  • Glyoxylate (to residues corresponding to position 427)
  • Acetyl-CoA; via carbonyl oxygen (to residues corresponding to position 536)

Modified residuei

  • Cysteine sulfenic acid (-SOH) (to residues corresponding to position 617)
  • Cysteine sulfenic acid (-SOH) (to residues corresponding to position 688)

Regioni

  • Acetyl-CoA binding (to residues corresponding to positions 125 - 126)
  • Glyoxylate binding (to residues corresponding to positions 452 - 455)

Keywordsi

GO (Gene Ontology) termsi

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