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The annotation and conditions in this rule are derived from the following entries: Q58819 (ENDA_METJA)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_01833
    • taxon = Archaea
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 115 - 115 aligns to "Y" in entry Q58819 (individually applies "")
    • Subsequence at position 125 - 125 aligns to "H" in entry Q58819 (individually applies "")
    • Subsequence at position 156 - 156 aligns to "K" in entry Q58819 (individually applies "")

... then these annotations are applied i

Protein namei

  • Recommended name:
    tRNA-splicing endonuclease (EC:
    Alternative name(s):
    tRNA-intron endonuclease

Gene namei

  • Name:endA

Sequence similaritiesi

Catalytic activityi

  • PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.


  • Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.

Subunit structurei

  • Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.

Active sitei

  • (to residues corresponding to position 115)
  • (to residues corresponding to position 125)
  • (to residues corresponding to position 156)


GO (Gene Ontology) termsi

  • GO:0000213 tRNA-intron endonuclease activity
  • GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation
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