Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

The annotation and conditions in this rule are derived from the following entries: P0A6K1 (DAPF_ECOLI), P44859 (DAPF_HAEIN), Q8NP73 (DAPF_CORGL), P9WP19 (DAPF_MYCTU)

If a protein meets these conditions... i

Common conditions

Special conditions

    • Subsequence at position 11 - 11 aligns to "N" in entry P44859 (individually applies "Substrate")
    • Subsequence at position 44 - 44 aligns to "Q" in entry P44859 (individually applies "Substrate")
    • Subsequence at position 64 - 64 aligns to "[NQ]" in entry P44859 (individually applies "Substrate")
    • Subsequence at position 73 - 73 aligns to "C" in entry P44859 (individually applies "Proton donor")
    • Subsequence at position 74 - 75 aligns to "[GI]-N" in entry P44859 (individually applies "Substrate binding")
    • Subsequence at position 157 - 157 aligns to "N" in entry P44859 (individually applies "Substrate")
    • Subsequence at position 159 - 159 aligns to "H" in entry P44859 (individually applies "Could be important to modulate the pK values of the two catalytic cysteine residues")
    • Subsequence at position 190 - 190 aligns to "N" in entry P44859 (individually applies "Substrate")
    • Subsequence at position 208 - 208 aligns to "E" in entry P44859 (individually applies "Could be important to modulate the pK values of the two catalytic cysteine residues")
    • Subsequence at position 208 - 209 aligns to "E-R" in entry P44859 (individually applies "Substrate binding")
    • Subsequence at position 217 - 217 aligns to "C" in entry P44859 (individually applies "Proton acceptor")
    • Subsequence at position 218 - 219 aligns to "G-[ST]" in entry P44859 (individually applies "Substrate binding")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Diaminopimelate epimerase (EC:5.1.1.7)
    Short name:
    DAP epimerase
    Alternative name(s):
    PLP-independent amino acid racemase

Gene namei

  • Name:dapF

Pathwayi

  • Pathwayi: L-lysine biosynthesis via DAP pathway

    This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate. This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Catalytic activityi

  • LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.

Subunit structurei

  • Homodimer.

Sequence similaritiesi

Subcellular locationi

Functioni

  • Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine.
  • Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.

Binding sitei

  • Substrate (to residues corresponding to position 11)
  • Substrate (to residues corresponding to position 44)
  • Substrate (to residues corresponding to position 64)
  • Substrate (to residues corresponding to position 157)
  • Substrate (to residues corresponding to position 190)

Sitei

  • Could be important to modulate the pK values of the two catalytic cysteine residues (to residues corresponding to position 159)
  • Could be important to modulate the pK values of the two catalytic cysteine residues (to residues corresponding to position 208)
  • Important for dimerization (to residues corresponding to position 268)

Regioni

  • Substrate binding (to residues corresponding to positions 74 - 75)
  • Substrate binding (to residues corresponding to positions 208 - 209)
  • Substrate binding (to residues corresponding to positions 218 - 219)

Active sitei

  • Proton donor (to residues corresponding to position 73)
  • Proton acceptor (to residues corresponding to position 217)

Keywordsi

GO (Gene Ontology) termsi

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health