Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

The annotation and conditions in this rule are derived from the following entries: P07649 (TRUA_ECOLI)

If a protein meets these conditions... i

Common conditions

Special conditions

  • Subsequence at position 58 - 58 aligns to "R" in entry P07649 (applies "Interaction with tRNA. Important for base-flipping") Subsequence at position 78 - 78 aligns to "R" in entry P07649 (applies "Interaction with tRNA") Subsequence at position 107 - 111 aligns to "F-H-A-R-F" in entry P07649 (applies "RNA binding") Subsequence at position 110 - 110 aligns to "R" in entry P07649 (applies "Interaction with tRNA") Subsequence at position 126 - 126 aligns to "R" in entry P07649 (applies "Interaction with tRNA") Subsequence at position 139 - 139 aligns to "F" in entry P07649 (applies "Interaction with tRNA") Subsequence at position 168 - 172 aligns to "Q-C-Q-S-R" in entry P07649 (applies "Interaction with tRNA")
    • Subsequence at position 60 - 60 aligns to "D" in entry P07649 (individually applies "Nucleophile")
    • Subsequence at position 118 - 118 aligns to "Y" in entry P07649 (individually applies "Substrate")

... then these annotations are applied i

Protein namei

  • Recommended name:
    tRNA pseudouridine synthase A (EC:5.4.99.12)
    Alternative name(s):
    tRNA-uridine isomerase I
    tRNA pseudouridine(38-40) synthase
    tRNA pseudouridylate synthase I

Gene namei

  • Name:truA

Catalytic activityi

  • tRNA uridine(38-40) = tRNA pseudouridine(38-40).

Functioni

  • Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.

Subunit structurei

  • Homodimer.

Sequence similaritiesi

Sitei

  • Interaction with tRNA. Important for base-flipping (to residues corresponding to position 58)
  • Interaction with tRNA (to residues corresponding to position 78)
  • Interaction with tRNA (to residues corresponding to position 110)
  • Interaction with tRNA (to residues corresponding to position 126)
  • Interaction with tRNA (to residues corresponding to position 139)

Binding sitei

  • Substrate (to residues corresponding to position 118)

Active sitei

  • Nucleophile (to residues corresponding to position 60)

Regioni

  • RNA binding (to residues corresponding to positions 107 - 111)
  • Interaction with tRNA (to residues corresponding to positions 168 - 172)

Keywordsi

GO (Gene Ontology) termsi

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again