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The annotation and conditions in this rule are derived from the following entries: P05523 (FPG_ECOLI), P42371 (FPG_LACLC), O50606 (FPG_THET8)

If a protein meets these conditions... i

Common conditions

    • Matches HAMAP signature MF_00103
    • taxon = Bacteria
    • fragment ≠ the sequence is fragmented

Special conditions

    • Subsequence at position 2 - 2 aligns to "P" in entry P05523 (individually applies "Schiff-base intermediate with DNA")
    • Subsequence at position 3 - 3 aligns to "E" in entry P05523 (individually applies "Proton donor")
    • Subsequence at position 57 - 57 aligns to "K" in entry P05523 (individually applies "Proton donor; for beta-elimination activity")
    • Subsequence at position 90 - 90 aligns to "H" in entry P05523 (individually applies "DNA")
    • Subsequence at position 109 - 109 aligns to "R" in entry P05523 (individually applies "DNA")
    • Subsequence at position 150 - 150 aligns to "[KR]" in entry P05523 (individually applies "DNA")
    • Subsequence at position 259 - 259 aligns to "R" in entry P05523 (individually applies "Proton donor; for delta-elimination activity")

... then these annotations are applied i

Protein namei

  • Recommended name:
    Formamidopyrimidine-DNA glycosylase (EC:3.2.2.23)
    Short name:
    Fapy-DNA glycosylase
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyase MutM (EC:4.2.99.18)
    Short name:
    AP lyase MutM

Gene namei

  • Name:mutM, Synonym:fpg

Sequence similaritiesi

Subunit structurei

  • Monomer.

Cofactori

  • Zn2+Note: Binds 1 zinc ion per subunit.

Functioni

  • Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Catalytic activityi

  • Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
  • The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Active sitei

  • Schiff-base intermediate with DNA (to residues corresponding to position 2)
  • Proton donor (to residues corresponding to position 3)
  • Proton donor; for beta-elimination activity (to residues corresponding to position 57)
  • Proton donor; for delta-elimination activity (to residues corresponding to position 259)

Binding sitei

  • DNA (to residues corresponding to position 90)
  • DNA (to residues corresponding to position 109)
  • DNA (to residues corresponding to position 150)

Keywordsi

GO (Gene Ontology) termsi

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