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Protein

D-alanine--D-alanyl carrier protein ligase

Gene

dltA

Organism
Staphylococcus aureus
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.UniRule annotationSAAS annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

D-alanine + ATP + holo-[D-alanyl-carrier protein] = AMP + diphosphate + D-alanyl-[D-alanyl-carrier protein].UniRule annotationSAAS annotation

Pathwayi: lipoteichoic acid biosynthesis

This protein is involved in the pathway lipoteichoic acid biosynthesis, which is part of Cell wall biogenesis.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway lipoteichoic acid biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189D-alanineUniRule annotation1
Binding sitei293D-alanine; via carbonyl oxygenUniRule annotation1
Binding sitei365ATPUniRule annotation1
Binding sitei473ATPUniRule annotation1
Binding sitei473D-alanineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 145ATPUniRule annotation2
Nucleotide bindingi284 – 289ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotationImported
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00556

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanyl carrier protein ligaseUniRule annotation (EC:6.2.1.-UniRule annotation)
Short name:
DCLUniRule annotation
Alternative name(s):
D-alanine--poly(phosphoribitol) ligase subunit 1UniRule annotation
D-alanine-activating enzymeUniRule annotation
Short name:
DAEUniRule annotation
Gene namesi
Name:dltAUniRule annotationImported
ORF Names:BTN44_08160Imported, C3B39_04425Imported, EQ90_04305Imported, HMPREF3211_01980Imported
OrganismiStaphylococcus aureusImported
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000070177 Componenti: Unassembled WGS sequence
  • UP000237348 Componenti: Unassembled WGS sequence
  • UP000236484 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 386AMP-bindingInterPro annotationAdd BLAST378
Domaini395 – 473AMP-binding_CInterPro annotationAdd BLAST79

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily.UniRule annotationSAAS annotation

Family and domain databases

HAMAPiMF_00593 DltA, 1 hit
InterProiView protein in InterPro
IPR010071 AA_adenyl_domain
IPR025110 AMP-bd_C
IPR000873 AMP-dep_Synth/Lig
IPR010072 DltA
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR01733 AA-adenyl-dom, 1 hit
TIGR01734 D-ala-DACP-lig, 1 hit

Sequencei

Sequence statusi: Complete.

X5EMW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIINKLQA FADANPQSIA VRHTTDELTY QQLMDESSKL AHRLQGSKKP
60 70 80 90 100
MILFGHMSPY MIVGMIGAIK AGCGYVPVDT SIPEDRIKMI INKVQPEFVF
110 120 130 140 150
NTTDESFESL EGEVFTIEDI KTSQDPVIFD SQIKDNDTVY TIFTSGSTGE
160 170 180 190 200
PKGVQIEYAS LVQFTEWMLE LNKSGNEQQW LNQAPFSFDL SVMAIYPCLA
210 220 230 240 250
SGGTLNLVDK NMINKPKLLN EMLTATPINI WVSTPSFMEM CLLLPTLNEE
260 270 280 290 300
QYGSLNEFFF CGEILPHRAA KALVNRFPSA TIYNTYGPTE ATVAVTSIQI
310 320 330 340 350
TQEILDQYPT LPVGVERPGA RLSTTDEGEL VIEGQSVSLG YLKNDQKTAE
360 370 380 390 400
VFNFDDGIRT YHTGDKAKFE NGQWFIQGRI DFQIKLNGYR MELEEIETQL
410 420 430 440 450
RQSEFVKEAI VVPVYKNDKV IHLIGAIVPT TEVTDNAEMT KNIKNDLKSR
460 470 480
LPEYMIPRKF EWMEQLPLTS NGKIDRKKIA EVING
Length:485
Mass (Da):54,670
Last modified:June 11, 2014 - v1
Checksum:iFFA535607F1FD328
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP018629 Genomic DNA Translation: AUS75211.1
LALJ01000006 Genomic DNA Translation: KMR37401.1
LRQH01000146 Genomic DNA Translation: KXA34243.1
PQBH01000002 Genomic DNA Translation: POH56153.1
RefSeqiWP_000129653.1, NZ_PYFI01000001.1

Genome annotation databases

EnsemblBacteriaiCFF98890; CFF98890; ERS093009_00357
CXE18884; CXE18884; ERS074020_00104
CXL46552; CXL46552; ERS072738_00109
CZQ25806; CZQ25806; ERS1058648_00233
KXA34243; KXA34243; HMPREF3211_01980
OAP80407; OAP80407; A4U86_00805
SCR11086; SCR11086; SAMEA2298760_00779
PATRICifig|1280.10758.peg.803

Similar proteinsi

Entry informationi

Entry nameiX5EMW7_STAAU
AccessioniPrimary (citable) accession number: X5EMW7
Entry historyiIntegrated into UniProtKB/TrEMBL: June 11, 2014
Last sequence update: June 11, 2014
Last modified: July 18, 2018
This is version 47 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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