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Entry version 60 (02 Jun 2021)
Sequence version 1 (11 Jun 2014)
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Protein

E3 ubiquitin protein ligase

Gene

Bre1

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.UniRule annotationARBA annotation EC:2.3.2.27

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.UniRule annotationARBA annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulatorUniRule annotationARBA annotation, TransferaseUniRule annotation
Biological processUbl conjugation pathwayUniRule annotation
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00143

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
E3 ubiquitin protein ligaseUniRule annotation (EC:2.3.2.27UniRule annotation)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Bre1Imported
Synonyms:Bre-1Imported, bre-1Imported, BRE1Imported, bre1Imported, dBRE1Imported, dBre1Imported, Dmel\CG10542Imported, EC3-8Imported, l(3)01640Imported
ORF Names:CG10542Imported, Dmel_CG10542Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3L

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0086694, Bre1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

NucleusUniRule annotation

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0086694, Expressed in embryo and 36 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
X2JGB3, baseline and differential

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
X2JGB3

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini991 – 1030RING-typeInterPro annotationAdd BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni182 – 214DisorderedSequence analysisAdd BLAST33
Regioni312 – 335DisorderedSequence analysisAdd BLAST24
Regioni546 – 586DisorderedSequence analysisAdd BLAST41
Regioni615 – 664DisorderedSequence analysisAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili61 – 81Sequence analysisAdd BLAST21
Coiled coili255 – 310Sequence analysisAdd BLAST56
Coiled coili337 – 382Sequence analysisAdd BLAST46
Coiled coili422 – 442Sequence analysisAdd BLAST21
Coiled coili496 – 530Sequence analysisAdd BLAST35
Coiled coili664 – 691Sequence analysisAdd BLAST28
Coiled coili705 – 762Sequence analysisAdd BLAST58
Coiled coili807 – 827Sequence analysisAdd BLAST21
Coiled coili842 – 890Sequence analysisAdd BLAST49
Coiled coili912 – 932Sequence analysisAdd BLAST21
Coiled coili940 – 967Sequence analysisAdd BLAST28

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi561 – 586Polar residuesSequence analysisAdd BLAST26
Compositional biasi633 – 648Polar residuesSequence analysisAdd BLAST16
Compositional biasi649 – 664Basic and acidic residuesSequence analysisAdd BLAST16

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the BRE1 family.UniRule annotationARBA annotation

Keywords - Domaini

Coiled coilUniRule annotationARBA annotation, Zinc-fingerPROSITE-ProRule annotationARBA annotation

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000002866

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_002640_0_0_1

Identification of Orthologs from Complete Genome Data

More...
OMAi
NDQKEMK

Database of Orthologous Groups

More...
OrthoDBi
782448at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
X2JGB3

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.40.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013956, E3_ubiquit_lig_Bre1
IPR018957, Znf_C3HC4_RING-type
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS

The PANTHER Classification System

More...
PANTHERi
PTHR23163, PTHR23163, 4 hits

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00097, zf-C3HC4, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00184, RING, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequence (1+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

X2JGB3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKRSADDAT GSSCLVAAAA AGQPPIKKVH FEPHLIGPVS TLEEMDIKVL
60 70 80 90 100
EFQNKKLAQR IEQRMRTEAE LRHRIEQLEK RQTQDDAVLN VVNRYWNQLN
110 120 130 140 150
EDIRVLLQRF DAETADELEN RNENEVTTSF LAQLSTWDKE ELDEKLANRV
160 170 180 190 200
QVSKRAVAKI VQVIDRLMQR NEKITHVLKG DSLASAGSGS GAGAGGEEEQ
210 220 230 240 250
QQASGDAETT TSSAGVHALE ETLKQTHIEI MSENHKLQNL NTSLHEKFHT
260 270 280 290 300
MSLKMKEYQD AHTAKETENA ELKNQIDELQ YDLEKIHCRN DKLENHLAEA
310 320 330 340 350
IEKLKAYHQI YGDPNKSTNS AKTPTTTGSG GATTSVNSQL LEELQKELEE
360 370 380 390 400
YRELANNRLQ ELDKLHATHR ETLKEVEKLK MDIRQLPESV IVETTEYKCL
410 420 430 440 450
QSQFSVLYNE SMQIKTMLDE TRNQLQTSKN QHLRQIEVME SEELIAQKKV
460 470 480 490 500
RSEMIQMEDV LALIRKEYET LRIEFEQNMA ANEQTAPINR EMRHLITSLQ
510 520 530 540 550
NHNGQLKGEV QRYKRKYKDT STDNLKLRQE LADALATLEG NKLQAATGAA
560 570 580 590 600
GEEIKQENST GVKEENSNNV SASGQTNQTN SGNDTNVAIK EENHISAEDE
610 620 630 640 650
ADDEASGKDV KDGIKQEKLS SGDAAAAEKK DSPGPGNSTS SATNSVPVKN
660 670 680 690 700
EKDSKDGVKG KDVKAVESET VRDLKAQLKK ALNDQKEMKL LLDMYKGVSK
710 720 730 740 750
DQRDKVQLMA TEKKLRSEIE ELRQQLKKLQ ESKREERKKL ADEEALRKIK
760 770 780 790 800
QLEEQKYELQ KQMANHKPTD NSWGSGAPGT ANYTRPFVGS HEEEALLNEM
810 820 830 840 850
EVTGQAFEDM QEQNSRLIQQ LREKDDANFK LMSERIKANQ LHKLLREEKT
860 870 880 890 900
VLEDQMATAT TQIEAMHIVL RKLEEKERSL QATVASIEKE LMLRQQAMEM
910 920 930 940 950
HKRKAIESAQ SAADLKLHLE KYHAQMKEAQ QVVAEKTSSL EAEAYKTKRL
960 970 980 990 1000
QEELAQFKRK AERMKKMEMS GTTIDEVMIE EIREYKETLT CPSCKVKRKD
1010 1020 1030 1040
AVLSKCFHVF CYDCLRTRYE TRQRKCPKCN CAFGANDYHR LYLQ
Length:1,044
Mass (Da):119,060
Last modified:June 11, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i426B02E6C90B37BC
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q9VRP9BRE1_DROME
E3 ubiquitin-protein ligase Bre1
Bre1 CG10542
1,044Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AHN57975.1

NCBI Reference Sequences

More...
RefSeqi
NP_001286950.1, NM_001300021.1
NP_647989.2, NM_139732.3

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0077116; FBpp0076822; FBgn0086694
FBtr0344538; FBpp0310889; FBgn0086694

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
38652

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG10542

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA Translation: AHN57975.1
RefSeqiNP_001286950.1, NM_001300021.1
NP_647989.2, NM_139732.3

3D structure databases

SMRiX2JGB3
ModBaseiSearch...

Protocols and materials databases

The DNASU plasmid repository

More...
DNASUi
38652

Genome annotation databases

EnsemblMetazoaiFBtr0077116; FBpp0076822; FBgn0086694
FBtr0344538; FBpp0310889; FBgn0086694
GeneIDi38652
KEGGidme:Dmel_CG10542

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
38652
FlyBaseiFBgn0086694, Bre1

Phylogenomic databases

GeneTreeiENSGT00390000002866
HOGENOMiCLU_002640_0_0_1
OMAiNDQKEMK
OrthoDBi782448at2759
PhylomeDBiX2JGB3

Enzyme and pathway databases

UniPathwayiUPA00143

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...
BioGRID-ORCSi
38652, 1 hit in 3 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
Bre1, fly

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
38652

Gene expression databases

BgeeiFBgn0086694, Expressed in embryo and 36 other tissues
ExpressionAtlasiX2JGB3, baseline and differential

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013956, E3_ubiquit_lig_Bre1
IPR018957, Znf_C3HC4_RING-type
IPR001841, Znf_RING
IPR013083, Znf_RING/FYVE/PHD
IPR017907, Znf_RING_CS
PANTHERiPTHR23163, PTHR23163, 4 hits
PfamiView protein in Pfam
PF00097, zf-C3HC4, 1 hit
SMARTiView protein in SMART
SM00184, RING, 1 hit
PROSITEiView protein in PROSITE
PS00518, ZF_RING_1, 1 hit
PS50089, ZF_RING_2, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiX2JGB3_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: X2JGB3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: June 11, 2014
Last sequence update: June 11, 2014
Last modified: June 2, 2021
This is version 60 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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