UniProtKB - X2JAI4 (X2JAI4_DROME)
DNA replication licensing factor MCM3
Mcm3
Functioni
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
UniRule annotationCatalytic activityi
- EC:3.6.4.12UniRule annotationARBA annotation
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: UniProtKB-UniRule
GO - Biological processi
- DNA replication initiation Source: InterPro
- mushroom body development Source: FlyBase
- neuroblast proliferation Source: FlyBase
Keywordsi
Molecular function | DNA-bindingUniRule annotationARBA annotation, HelicaseUniRule annotationARBA annotation, Hydrolase |
Biological process | DNA replicationUniRule annotationARBA annotation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: DNA replication licensing factor MCM3UniRule annotation (EC:3.6.4.12UniRule annotation) |
Gene namesi | Name:Mcm3Imported Synonyms:dMCM3Imported, dMcm3Imported, Dmel\CG4206Imported, DmeMCM3Imported, DmMCM3Imported, DmMcm3Imported, MCM3Imported, McM3Imported, mcm3Imported, Mcp-PCR2Imported, PCR2Imported, smuImported ORF Names:CG4206Imported, Dmel_CG4206Imported |
Organismi | Drosophila melanogaster (Fruit fly)Imported |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0284442, Mcm3 |
VEuPathDBi | VectorBase:FBgn0284442 |
Subcellular locationi
Nucleus
- Nucleus UniRule annotationARBA annotation
Nucleus
- CMG complex Source: UniProt
Other locations
- MCM complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
NucleusUniRule annotationARBA annotationExpressioni
Gene expression databases
Bgeei | FBgn0024332, Expressed in embryo and 33 other tissues |
ExpressionAtlasi | X2JAI4, baseline and differential |
Interactioni
Subunit structurei
Component of the MCM2-7 complex.
UniRule annotationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 290 – 496 | MCMInterPro annotationAdd BLAST | 207 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 654 – 716 | DisorderedSequence analysisAdd BLAST | 63 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 654 – 680 | Basic and acidic residuesSequence analysisAdd BLAST | 27 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT01040000240460 |
HOGENOMi | CLU_000995_6_0_1 |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAHEGEQFIK DIQREYVDFL DDEEDQGIYA GHVKDMIAEK SKRLIVNVND
60 70 80 90 100
LKRKNPQRAL GLLSNAADEQ LAFGRALKEY ASTVDPGYAK MHEDLFVGFE
110 120 130 140 150
GCFGNRHVTP RSLTSIYLGN MVCVEGIVTK VSLIRPKVVR SVHYCPNTRK
160 170 180 190 200
VMERKYTDLT SFEAVPSGAA YPTKDEDGNL LETEYGLSVY KDHQTLTIQE
210 220 230 240 250
MPEKAPAGQL PRSVDIVCDD DLVDRCKPGD RVQIVGSYRC LPGKRGGYTS
260 270 280 290 300
GTFRTVLLAN NISLLSKESN LDISREDIML CKKLAKNNDI FELLSKSLAP
310 320 330 340 350
SIHGHAYVKQ AILCLLLGGV EKILPNGTRL RGDINVLLIG DPSVAKSQLL
360 370 380 390 400
RYVLNTAPRA IPTTGRGSSG VGLTAAVTTD QETGERRLEA GAMVLADRGV
410 420 430 440 450
VCIDEFDKMS DIDRTAIHEV MEQGRVTISK AGIHASLNAR CSVLAAANPV
460 470 480 490 500
YGRYDQYKTP MENIGLQDSL LSRFDLLFVM LDVIDSDVDQ MISDHVVRMH
510 520 530 540 550
RYRNPKEADG EPLSMGSSYA DSLSFVSSSE EKDTEVYEKY DALLHGKSRQ
560 570 580 590 600
RHEKILSVEF MRKYIHIAKC MKPKLGEQAC EAIANEYSRL RSQEAVETDV
610 620 630 640 650
ARTQPITART LETLIRLSTA HARARMSKSV TIDDAHAAIE LVQFAYFKKV
660 670 680 690 700
LDKDRPSKRR RNSGSDAEDD NGEASSQRSP SRRSKRTRTA TVGADSDEED
710 720 730 740 750
IEPPQPDAGD LTRRETRRSL PARSVAMLMA SPSSEEQSVA TSTTEPAIIS
760 770 780 790 800
DARLGEFKNN LQRLFREARE QSLALARITT AINVGSQEPF TAGEIEAAVH
810
RMTEDNQIMV ADDIVFLI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ9XYU1 | MCM3_DROME | DNA replication licensing factor Mc... | Mcm3 Mcm3-RA, CG4206 | 819 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AHN59355.1 |
RefSeqi | NP_001284884.1, NM_001297955.1 |
Genome annotation databases
EnsemblMetazoai | FBtr0340476; FBpp0309417; FBgn0284442 |
GeneIDi | 31449 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE014298 Genomic DNA Translation: AHN59355.1 |
RefSeqi | NP_001284884.1, NM_001297955.1 |
3D structure databases
SMRi | X2JAI4 |
ModBasei | Search... |
Proteomic databases
PRIDEi | X2JAI4 |
Genome annotation databases
EnsemblMetazoai | FBtr0340476; FBpp0309417; FBgn0284442 |
GeneIDi | 31449 |
Organism-specific databases
CTDi | 4172 |
FlyBasei | FBgn0284442, Mcm3 |
VEuPathDBi | VectorBase:FBgn0284442 |
Phylogenomic databases
GeneTreei | ENSGT01040000240460 |
HOGENOMi | CLU_000995_6_0_1 |
Miscellaneous databases
BioGRID-ORCSi | 31449, 0 hits in 1 CRISPR screen |
GenomeRNAii | 31449 |
Gene expression databases
Bgeei | FBgn0024332, Expressed in embryo and 33 other tissues |
ExpressionAtlasi | X2JAI4, baseline and differential |
Family and domain databases
Gene3Di | 2.40.50.140, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR031327, MCM IPR008046, Mcm3 IPR018525, MCM_CS IPR001208, MCM_dom IPR041562, MCM_lid IPR027925, MCM_N IPR033762, MCM_OB IPR012340, NA-bd_OB-fold IPR027417, P-loop_NTPase |
PANTHERi | PTHR11630, PTHR11630, 1 hit |
Pfami | View protein in Pfam PF00493, MCM, 1 hit PF17855, MCM_lid, 1 hit PF14551, MCM_N, 1 hit PF17207, MCM_OB, 1 hit |
PRINTSi | PR01657, MCMFAMILY PR01659, MCMPROTEIN3 |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00350, MCM, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00847, MCM_1, 1 hit PS50051, MCM_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | X2JAI4_DROME | |
Accessioni | X2JAI4Primary (citable) accession number: X2JAI4 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | June 11, 2014 |
Last sequence update: | June 11, 2014 | |
Last modified: | May 25, 2022 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |