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Protein

Lipoyl synthase, mitochondrial

Gene

COCMIDRAFT_88806

Organism
Bipolaris oryzae ATCC 44560
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Octanoyltransferase (COCMIDRAFT_3003)
  2. Lipoyl synthase, mitochondrial (COCMIDRAFT_88806)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi129Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi134Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi140Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi160Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi164Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi167Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferaseUniRule annotation
Ligand4Fe-4SUniRule annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
ORF Names:COCMIDRAFT_88806Imported
OrganismiBipolaris oryzae ATCC 44560Imported
Taxonomic identifieri930090 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris
Proteomesi
  • UP000054032 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

MitochondrionUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliW6ZVT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 359Elp3InterPro annotationAdd BLAST210

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

KOiK03644

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDF00271 lipoyl_synthase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

W6ZVT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASIPRTRC LAALKVVPRS TAPLRSFATT SDAPQPPASE TPSKRSRPAT
60 70 80 90 100
SFSDTLNAGP SFGDFVNPNA PLSPAEAYEI KTVQVGPEGR KKTITRLPEW
110 120 130 140 150
LRTPIPSNAN QNYKQIKKDL RGLNLATVCE EARCPNISDC WGGSSKSAAT
160 170 180 190 200
ATIMLMGDTC TRGCRFCAVK TSKAPPPLDP HEPENTAEAL RRWGLGYVVI
210 220 230 240 250
TVVDRDDLAD SGAHHIAETI MKIKQKNPTQ LVELLGGDYG GNLEMAKVVA
260 270 280 290 300
RSGVDVFAHN IETTERLTPF VRDRRAKFRQ SLDVLRSAKE AQPELITKTS
310 320 330 340 350
MMLGLGETDE DVWHALRELR ANGVDVVTFG QYMRPTKRHL AVHEYVTPDK
360 370 380 390 400
FELWRQRALD MGFLYCASGP LVRSSYKAGE AFIENVLKKR RLGTSAETKV
410
AELNTAEEAG KAI
Length:413
Mass (Da):45,364
Last modified:April 16, 2014 - v1
Checksum:i7365B6F1DBF390EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KI963947 Genomic DNA Translation: EUC47901.1
RefSeqiXP_007685624.1, XM_007687434.1

Genome annotation databases

EnsemblFungiiEUC47901; EUC47901; COCMIDRAFT_88806
GeneIDi19127348
KEGGibor:COCMIDRAFT_88806

Similar proteinsi

Entry informationi

Entry nameiW6ZVT2_COCMI
AccessioniPrimary (citable) accession number: W6ZVT2
Entry historyiIntegrated into UniProtKB/TrEMBL: April 16, 2014
Last sequence update: April 16, 2014
Last modified: July 18, 2018
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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