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Protein

Dihydroxy-acid dehydratase

Gene

ilvD

Organism
Mannheimia sp. USDA-ARS-USMARC-1261
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (X781_4940), Acetolactate synthase (X781_12250)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (X781_5750)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (X781_4940), Acetolactate synthase (X781_12250)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (X781_5750)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi150Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi223Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotationSAAS annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00047; UER00057
UPA00049; UER00061

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxy-acid dehydrataseUniRule annotationSAAS annotation (EC:4.2.1.9UniRule annotationSAAS annotation)
Short name:
DADUniRule annotation
Gene namesi
Name:ilvDUniRule annotation
ORF Names:X781_7640Imported
OrganismiMannheimia sp. USDA-ARS-USMARC-1261Imported
Taxonomic identifieri1432056 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeMannheimia
Proteomesi
  • UP000019094 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01687

Family and domain databases

HAMAPiMF_00012 IlvD, 1 hit
InterProiView protein in InterPro
IPR004404 DihydroxyA_deHydtase
IPR000581 DiOHA_6PGluconate_deHydtase
IPR020558 DiOHA_6PGluconate_deHydtase_CS
IPR037237 IlvD/EDD_N
PfamiView protein in Pfam
PF00920 ILVD_EDD, 1 hit
SUPFAMiSSF143975 SSF143975, 1 hit
TIGRFAMsiTIGR00110 ilvD, 1 hit
PROSITEiView protein in PROSITE
PS00886 ILVD_EDD_1, 1 hit
PS00887 ILVD_EDD_2, 1 hit

Sequencei

Sequence statusi: Complete.

W0Q3S6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKNYRFPKN SSRINPFNKH NIKNLEKIMP ILRSATSTQG RNMAGARALW
60 70 80 90 100
RATGMKEGDF GKPIIAVVNS FTQFVPGHVH LKDMGQLVAA EIEKAGGVAK
110 120 130 140 150
EFNTIAVDDG IAMGHGGMLY SLPSRDLIAD SVEYMVNAHC ADAMVCISNC
160 170 180 190 200
DKITPGMLMA AMRLNIPTIF VSGGPMEAGK TKLSDQLIRL DLVDAMIESA
210 220 230 240 250
DPNVSDERID AIEKHACPTC GSCSGMFTAN SMNCLTEALG LSLPGNGSML
260 270 280 290 300
ATHADRKELF LKAGREIVEL CRKYYQEDDE SVLPRSIASF DAFENAMSLD
310 320 330 340 350
IAMGGSSNTV LHLLAAAQEA GVDFKMADID RLSRVVPCLS KIAPNTNKYH
360 370 380 390 400
MEDVHRAGGI MGLLGELDRA GLIHRHVKTV LGLTIEEQLN QYDIIRNKDE
410 420 430 440 450
ELHKFFRAGP AGIRTTKAFS QDCRWDTVDD DRENGCIRNV ENAVTKEGGL
460 470 480 490 500
AVLFGNIAED GCIVKTAGVD ESIWKFTGEA IVFESQEDAV AGILGGKVKE
510 520 530 540 550
GHIVIIRYEG PKGGPGMQEM LYPTSYLKSM GLGKKCALLT DGRFSGGTSG
560 570 580 590 600
LSIGHASPEA ASGGAIALIN DGDTVSIDIP NRSINLMISD EELAERRKEM
610 620 630 640
EARGDKAWQP VNRKREVSFA LKVFGHFATS ADKGAVRDKS LLK
Length:643
Mass (Da):69,676
Last modified:March 19, 2014 - v1
Checksum:i8F7344C051290FAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP006942 Genomic DNA Translation: AHG72912.1

Genome annotation databases

EnsemblBacteriaiAHG72912; AHG72912; X781_7640
KEGGimvr:X781_7640
PATRICifig|1432056.3.peg.769

Similar proteinsi

Entry informationi

Entry nameiW0Q3S6_9PAST
AccessioniPrimary (citable) accession number: W0Q3S6
Entry historyiIntegrated into UniProtKB/TrEMBL: March 19, 2014
Last sequence update: March 19, 2014
Last modified: November 22, 2017
This is version 24 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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