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Entry version 42 (16 Oct 2019)
Sequence version 1 (19 Feb 2014)
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Protein

Magnetosome protein MamB

Gene

mamB

Organism
Magnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Plays a dual, essential role in magnetosome formation; required for magnetosome vesicle formation as well as biomineralization (Probable) (PubMed:29243866). Requires heterodimerization with MamM for stability (PubMed:22007638). Probably binds and transports iron (Probable). One of 7 genes (mamLQBIEMO) able to induce magnetosome membrane biogenesis; coexpression of mamLQRBIEMO in a deletion of the 17 gene mamAB operon restores magnetosome vesicle formation but not magnetite biosynthesis (PubMed:27286560).2 Publications3 Publications

Miscellaneous

This bacteria makes up to 60 cubo-octahedral magnetosomes of about 45 nm in diameter which contain membrane-bound crystals of magnetite (Fe3O4).1 Publication
Expression of just the minimal mamAB gene cluster (MGMSRv2__2365 to MGMSRv2__2381), including this gene, is sufficient to form a minimal magnetosome chain with small magnetite particles.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processBiomineralization, Ion transport, Iron transport, Transport
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
MGRY1430440:MGMSRV2_RS11710-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.4.7.3 the cation diffusion facilitator (cdf) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Magnetosome protein MamBCurated
Alternative name(s):
MM33.31 Publication
Probable iron transporter MamBCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:mamB1 Publication
Ordered Locus Names:MGMSRv2__2368
ORF Names:mgI499, MGR_4102
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMagnetospirillum gryphiswaldense (strain DSM 6361 / JCM 21280 / NBRC 15271 / MSR-1)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri431944 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeMagnetospirillum
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000018922 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 12CytoplasmicCuratedAdd BLAST12
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 83LumenalCuratedAdd BLAST50
Transmembranei84 – 104HelicalSequence analysisAdd BLAST21
Topological domaini105 – 112CytoplasmicCurated8
Transmembranei113 – 133HelicalSequence analysisAdd BLAST21
Topological domaini134 – 164LumenalCuratedAdd BLAST31
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 297CytoplasmicCuratedAdd BLAST112

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Magnetosome, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

The most severe single mam gene deletion. Single gene disruption has no accumulation of magnetite, no intracellular magnetosome vesicles form, wild type levels of MamM, mislocation of MamC in 1-3 foci, MamI mislocalized in 1 to a few patches (PubMed:22007638, PubMed:27286560, PubMed:29243866). Deletion of approximately 80 kb of DNA, including this operon, leads to cells that are non-magnetic, lack internal membrane systems, grow poorly, have reduced mobility and take-up and accumulate iron poorly (PubMed:13129949).4 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi6C → S: Loss of magnetic response. 1 Publication1
Mutagenesisi9C → S: Loss of magnetic response. 1 Publication1
Mutagenesisi46H → A: Loss of magnetic response. 1 Publication1
Mutagenesisi50D → A: Loss of magnetic response. MamD is correctly localized, magnetosome vesicles form but are empty. 2 Publications1
Mutagenesisi50D → E: Loss of magnetic response. 1 Publication1
Mutagenesisi138C → A: Loss of magnetic response, decreased protein oligomerization. 1 Publication1
Mutagenesisi154D → A: Loss of magnetic response. 1 Publication1
Mutagenesisi158D → A: Loss of magnetic response. 1 Publication1
Mutagenesisi247D → A: About 30% fewer magnetite particles per cell, their size is normal. 1 Publication1
Mutagenesisi288 – 297Missing : About 60% magnetic response. 1 Publication10
Mutagenesisi293 – 297Missing : About 40% magnetic response. 1 Publication5

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004477361 – 297Magnetosome protein MamBAdd BLAST297

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified ‘at the protein level’.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Upon induction in a non-magnetic deletion mutant this protein is first found in the cell inner membrane, then collects into foci along the entire cell length from which magnetosome vesicle formation and subsequent clustering occur (at protein level).1 Publication

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Part of the probable 17 gene mamAB operon.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Forms homodimers via its C-terminal domain, may form higher order multimers that are sensitive to reducing agent. Probably interacts with MamE (Probable).

Interacts with MamM via their C-terminal domains (PubMed:22007638, PubMed:29243866).

1 Publication2 Publications

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
1430440.MGMSRv2_2368

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 214Transmembrane domain (TMD)1 PublicationAdd BLAST214
Regioni215 – 297C-terminal domain (CTD)1 PublicationAdd BLAST83

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The C-terminal domain (CTD) is probably responsible for hetero- and homodimerization and binds 1 Fe cation per subunit (Probable). The CTD assumes a V-shaped, dimeric metallo-chaperone-like fold (By similarity).By similarity1 Publication

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. [View classification]1 Publication

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

Database of Orthologous Groups

More...
OrthoDBi
381612at2

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.20.1510.10, 1 hit
3.30.70.1350, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR002524 Cation_efflux
IPR027470 Cation_efflux_CTD
IPR036837 Cation_efflux_CTD_sf
IPR027469 Cation_efflux_TMD_sf

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF01545 Cation_efflux, 1 hit
PF16916 ZT_dimer, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF160240 SSF160240, 1 hit
SSF161111 SSF161111, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01297 CDF, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

V6F510-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFENCRDCR EEVVWWAFTA DICMTLFKGI LGLMSGSVAL VADSLHSGAD
60 70 80 90 100
VVASGVTQLS LKISNKPADE RYPFGYGNIQ YISSAIVGSL LLIGASFLMY
110 120 130 140 150
GSVVKLISGT YEAPSIFAAL GASVTVIVNE LMYRYQICVG NENNSPAIIA
160 170 180 190 200
NAWDNRSDAI SSAAVMVGVI ASVIGFPIAD TIAAIGVSAL VGHIGLELIG
210 220 230 240 250
KAVHGLMDSS VDTELLQTAW QIATDTPLVH SIYFLRGRHV GEDVQFDIRL
260 270 280 290
RVDPNLRIKD SSMVAEAVRQ RIQDEIPHAR DIRLFVSPAP AAVTVRV
Length:297
Mass (Da):31,942
Last modified:February 19, 2014 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i1D03291B50B341FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti193H → R in AAL09999 (PubMed:11571158).Curated1
Sequence conflicti193H → R in CAJ30127 (PubMed:11571158).Curated1
Sequence conflicti193H → R in CAM78034 (PubMed:17449609).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF374354 Genomic DNA Translation: AAL09999.1
BX571797 Genomic DNA Translation: CAE12043.1
AM085146 Genomic DNA Translation: CAJ30127.1
CU459003 Genomic DNA Translation: CAM78034.1
HG794546 Genomic DNA Translation: CDK99583.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
CDK99583; CDK99583; MGMSRv2__2368

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
mgy:MGMSRv2__2368

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF374354 Genomic DNA Translation: AAL09999.1
BX571797 Genomic DNA Translation: CAE12043.1
AM085146 Genomic DNA Translation: CAJ30127.1
CU459003 Genomic DNA Translation: CAM78034.1
HG794546 Genomic DNA Translation: CDK99583.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

STRINGi1430440.MGMSRv2_2368

Protein family/group databases

TCDBi2.A.4.7.3 the cation diffusion facilitator (cdf) family

Genome annotation databases

EnsemblBacteriaiCDK99583; CDK99583; MGMSRv2__2368
KEGGimgy:MGMSRv2__2368

Phylogenomic databases

OrthoDBi381612at2

Enzyme and pathway databases

BioCyciMGRY1430440:MGMSRV2_RS11710-MONOMER

Family and domain databases

Gene3Di1.20.1510.10, 1 hit
3.30.70.1350, 1 hit
InterProiView protein in InterPro
IPR002524 Cation_efflux
IPR027470 Cation_efflux_CTD
IPR036837 Cation_efflux_CTD_sf
IPR027469 Cation_efflux_TMD_sf
PfamiView protein in Pfam
PF01545 Cation_efflux, 1 hit
PF16916 ZT_dimer, 1 hit
SUPFAMiSSF160240 SSF160240, 1 hit
SSF161111 SSF161111, 1 hit
TIGRFAMsiTIGR01297 CDF, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMAMB_MAGGM
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: V6F510
Secondary accession number(s): Q6NE50, Q93DY6
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 18, 2019
Last sequence update: February 19, 2014
Last modified: October 16, 2019
This is version 42 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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