UniProtKB - V6CLJ5 (LAG1_CAEEL)
Suppressor of hairless protein homolog
lag-1
Functioni
Transcriptional regulator that plays a central role in lin-12/Notch and glp-1/Notch signaling pathways, involved in cell-cell communication that regulate a broad spectrum of cell-fate determinations (PubMed:8625826).
Binds directly to the 5'-[A/G]TGGGAA-3' DNA consensus sequence, which is present in the regulatory region of several genes (PubMed:8625826, PubMed:18706403, PubMed:23615264, PubMed:15297877, PubMed:32196486, PubMed:21737278).
Acts as a transcriptional repressor when it is not associated with Notch proteins (By similarity).
When in a complex with a Notch intracellular domain (NICD) product of lin-12/Notch or glp-1/Notch, and transcription regulator lag-3, it may act as a transcriptional activator that activates transcription of target genes(PubMed:18381292, PubMed:10830967, PubMed:32196486, PubMed:9003776).
Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively (By similarity).
Autonomously required in the germline for the stem cell fate, acting in the glp-1-dependent transcriptional activation of genes, including lst-1 and sygl-1 (PubMed:32196486).
Involved in cell-fate specification during reproductive system development, by positively autoregulating its own expression, in response to lin-12/Notch signaling (PubMed:23615264, PubMed:32839181).
Plays a role in Notch-dependent induction of left-right asymmetry in interneurons and motoneurons (PubMed:21737278).
May repress expression of hlh-6, in a lin-12/Notch-independent manner (PubMed:18706403).
By similarity9 PublicationsGO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: InterPro
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- double-stranded DNA binding Source: WormBase
- Notch binding Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- sequence-specific DNA binding Source: UniProtKB
- transcription coactivator binding Source: UniProtKB
GO - Biological processi
- cell fate determination Source: UniProtKB
- cell fate specification Source: WormBase
- cell projection morphogenesis Source: UniProtKB
- dauer exit Source: WormBase
- germ-line stem cell division Source: WormBase
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- nematode larval development Source: WormBase
- Notch signaling pathway Source: WormBase
- oocyte growth Source: UniProtKB
- oviposition Source: WormBase
- positive regulation of production of miRNAs involved in gene silencing by miRNA Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of vulval development Source: WormBase
- regulation of cell fate specification Source: WormBase
- regulation of gene expression Source: UniProtKB
- regulation of mesodermal cell fate specification Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: UniProtKB
- stem cell fate determination Source: UniProtKB
Keywordsi
Molecular function | DNA-binding |
Biological process | Notch signaling pathway, Transcription, Transcription regulation |
Enzyme and pathway databases
SignaLinki | V6CLJ5 |
Names & Taxonomyi
Protein namesi | Recommended name: Suppressor of hairless protein homologCuratedAlternative name(s): CSL transcription factor lag-11 PublicationCurated Lin-12 and glp-1 phenotype proteinImported |
Gene namesi | Name:lag-1Imported ORF Names:K08B4.1Imported |
Organismi | Caenorhabditis elegansImported |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | K08B4.1a ; CE28839 ; WBGene00002245 ; lag-1 K08B4.1b ; CE25048 ; WBGene00002245 ; lag-1 K08B4.1c ; CE49374 ; WBGene00002245 ; lag-1 K08B4.1d ; CE49360 ; WBGene00002245 ; lag-1 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleus Source: WormBase
- RNA polymerase II transcription regulator complex Source: WormBase
Other locations
- chromatin Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 743 – 790 | Missing in q385; die as L1 larvae with cell transformations that result in the loss of the excretory cell, loss of the rectum and a twisted nose. 1 PublicationAdd BLAST | 48 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000452277 | 1 – 790 | Suppressor of hairless protein homologAdd BLAST | 790 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | WBGene00002245, Expressed in multi-cellular organism and 5 other tissues |
ExpressionAtlasi | V6CLJ5, baseline and differential |
Interactioni
Subunit structurei
Component of a complex consisting of at least a lin-12/Notch intracellular domain (NICD), lag-1, and lag-3 (PubMed:16530045, PubMed:10830967, PubMed:18381292). An NICD product of lin-12/Notch, including the RBP-j associated molecule (RAM) and ankyrin repeat (ANK) domains, interacts directly with lag-1 (PubMed:16530045, PubMed:10830967, PubMed:18381292, PubMed:9003776).
3 PublicationsGO - Molecular functioni
- Notch binding Source: UniProtKB
- transcription coactivator binding Source: UniProtKB
Protein-protein interaction databases
ComplexPortali | CPX-3152, CSL-Notch-Mastermind transcription factor complex |
IntActi | V6CLJ5, 15 interactors |
MINTi | V6CLJ5 |
STRINGi | 6239.K08B4.1a |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | V6CLJ5 |
SMRi | V6CLJ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 680 – 776 | IPT/TIGBy similarityAdd BLAST | 97 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 27 | DisorderedSequence analysisAdd BLAST | 27 | |
Regioni | 92 – 125 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 343 – 353 | DNA-binding2 PublicationsAdd BLAST | 11 | |
Regioni | 484 – 489 | DNA-binding2 Publications | 6 | |
Regioni | 514 – 519 | DNA-binding2 Publications | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3743, Eukaryota |
GeneTreei | ENSGT00390000005197 |
HOGENOMi | CLU_022207_1_0_1 |
OMAi | IQFQATQ |
OrthoDBi | 444988at2759 |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1450, 1 hit |
IDEALi | IID50096 |
InterProi | View protein in InterPro IPR015350, Beta-trefoil_DNA-bd_dom IPR036358, BTD_sf IPR040159, CLS_fam IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR008967, p53-like_TF_DNA-bd IPR015351, RBP-J/Cbf11/Cbf12_DNA-bd IPR037095, RBP-J/Cbf11_DNA-bd_sf IPR038007, RBP-Jkappa_IPT |
PANTHERi | PTHR10665, PTHR10665, 1 hit |
Pfami | View protein in Pfam PF09270, BTD, 1 hit PF09271, LAG1-DNAbind, 1 hit PF20144, TIG_SUH, 1 hit |
SMARTi | View protein in SMART SM01268, BTD, 1 hit SM01267, LAG1_DNAbind, 1 hit |
SUPFAMi | SSF110217, SSF110217, 1 hit SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
s (4)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MFSWRRGDCE SQKEENRSEE RKGEETIRFP TRSPFHCVLF LLTDGFVLHK
60 70 80 90 100
PTASPTVGFS PTIFSFYYSR WESSHRISHD ESGFCTAKTP LQDSTFTRHP
110 120 130 140 150
STSVPSSPST PRHSGMDYHQ SSSVASSEST ASTVAAAAAA AAAASLNQHH
160 170 180 190 200
HPHLYCDDGL LSRSLTDMVS SGGYDSSSSS LSAAASMCYP TPDAYYYHAP
210 220 230 240 250
PPPPPPQAQQ GFSSTDAWLQ MQMQPTYHNF GSTVVSTNSP LPSHLLSYGG
260 270 280 290 300
QPAFADPFYT IGQSTPNTSS FLDTSNSSFG APSTVVANPM TNYQLAFQAK
310 320 330 340 350
LGSLHSLIGD SVQSLTSDRM IDFLSNKEKY ECVISIFHAK VAQKSYGNEK
360 370 380 390 400
RFFCPPPCIY LIGQGWKLKK DRVAQLYKTL KASAQKDAAI ENDPIHEQQA
410 420 430 440 450
TELVAYIGIG SDTSERQQLD FSTGKVRHPG DQRQDPNIYD YCAAKTLYIS
460 470 480 490 500
DSDKRKYFDL NAQFFYGCGM EIGGFVSQRI KVISKPSKKK QSMKNTDCKY
510 520 530 540 550
LCIASGTKVA LFNRLRSQTV STRYLHVEGN AFHASSTKWG AFTIHLFDDE
560 570 580 590 600
RGLQETDNFA VRDGFVYYGS VVKLVDSVTG IALPRLRIRK VDKQQVILDA
610 620 630 640 650
SCSEEPVSQL HKCAFQMIDN ELVYLCLSHD KIIQHQATAI NEHRHQINDG
660 670 680 690 700
AAWTIISTDK AEYRFFEAMG QVANPISPCP VVGSLEVDGH GEASRVELHG
710 720 730 740 750
RDFKPNLKVW FGATPVETTF RSEESLHCSI PPVSQVRNEQ THWMFTNRTT
760 770 780 790
GDVEVPISLV RDDGVVYSSG LTFSYKSLER HGPCRIVSNY
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_060946 | 1 – 255 | MFSWR…QPAFA → MPLAYSTHDNFYESPKTPQP TWDHVHAQFPLGEPARNLDK FIVPEAMFQSVSPLAGVAAA PSQIAALQQIQALMTFQMQQ NNLFPKIDTISKSPTPELAS PSAKRMRLSPSTSSHSDVAS TSKGTNGQDQSKNSPTNS in isoform a. Add BLAST | 255 | |
Alternative sequenceiVSP_060947 | 1 – 255 | MFSWR…QPAFA → MPLAYSTHDNFYESPKTPQP TWDHVHAQFPLGEPARNLDK FIEAMFQSVSPLAGVAAAPS QIAALQQIQALMTFQMQQNN LFPKIDTISKSPTPELASPS AKRMRLSPSTSSHSDVASTS KGTNGQDQSKNSPTNS in isoform b. Add BLAST | 255 | |
Alternative sequenceiVSP_060948 | 1 – 255 | MFSWR…QPAFA → MFQSVSPLAGVAAAPSQIAA LQQIQALMTFQMQQNNLFPK IDTISKSPTPELASPSAKRM RLSPSTSSHSDVASTSKGTN GQDQSKNSPTNS in isoform c. Add BLAST | 255 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284604 Genomic DNA Translation: CCD72375.1 BX284604 Genomic DNA Translation: CCD72376.1 BX284604 Genomic DNA Translation: CDK13424.1 BX284604 Genomic DNA Translation: CDK13425.1 U49794 mRNA Translation: AAB03858.1 U49795 Genomic DNA Translation: AAB03859.1 |
PIRi | T33741 |
RefSeqi | NP_001023278.1, NM_001028107.3 [V6CLJ5-2] NP_001293739.1, NM_001306810.1 NP_001293740.1, NM_001306811.1 [V6CLJ5-1] NP_741410.1, NM_171350.4 [V6CLJ5-3] |
Genome annotation databases
EnsemblMetazoai | K08B4.1a.1; K08B4.1a.1; WBGene00002245 [V6CLJ5-3] K08B4.1b.1; K08B4.1b.1; WBGene00002245 [V6CLJ5-2] K08B4.1c.1; K08B4.1c.1; WBGene00002245 [V6CLJ5-4] K08B4.1d.1; K08B4.1d.1; WBGene00002245 [V6CLJ5-1] |
GeneIDi | 177373 |
KEGGi | cel:CELE_K08B4.1 |
UCSCi | K08B4.1a, c. elegans |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX284604 Genomic DNA Translation: CCD72375.1 BX284604 Genomic DNA Translation: CCD72376.1 BX284604 Genomic DNA Translation: CDK13424.1 BX284604 Genomic DNA Translation: CDK13425.1 U49794 mRNA Translation: AAB03858.1 U49795 Genomic DNA Translation: AAB03859.1 |
PIRi | T33741 |
RefSeqi | NP_001023278.1, NM_001028107.3 [V6CLJ5-2] NP_001293739.1, NM_001306810.1 NP_001293740.1, NM_001306811.1 [V6CLJ5-1] NP_741410.1, NM_171350.4 [V6CLJ5-3] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1TTU | X-ray | 2.85 | A | 309-780 | [»] | |
2FO1 | X-ray | 3.12 | A | 309-780 | [»] | |
3BRD | X-ray | 2.21 | A | 309-780 | [»] | |
AlphaFoldDBi | V6CLJ5 | |||||
SMRi | V6CLJ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3152, CSL-Notch-Mastermind transcription factor complex |
IntActi | V6CLJ5, 15 interactors |
MINTi | V6CLJ5 |
STRINGi | 6239.K08B4.1a |
Genome annotation databases
EnsemblMetazoai | K08B4.1a.1; K08B4.1a.1; WBGene00002245 [V6CLJ5-3] K08B4.1b.1; K08B4.1b.1; WBGene00002245 [V6CLJ5-2] K08B4.1c.1; K08B4.1c.1; WBGene00002245 [V6CLJ5-4] K08B4.1d.1; K08B4.1d.1; WBGene00002245 [V6CLJ5-1] |
GeneIDi | 177373 |
KEGGi | cel:CELE_K08B4.1 |
UCSCi | K08B4.1a, c. elegans |
Organism-specific databases
CTDi | 177373 |
WormBasei | K08B4.1a ; CE28839 ; WBGene00002245 ; lag-1 K08B4.1b ; CE25048 ; WBGene00002245 ; lag-1 K08B4.1c ; CE49374 ; WBGene00002245 ; lag-1 K08B4.1d ; CE49360 ; WBGene00002245 ; lag-1 |
Phylogenomic databases
eggNOGi | KOG3743, Eukaryota |
GeneTreei | ENSGT00390000005197 |
HOGENOMi | CLU_022207_1_0_1 |
OMAi | IQFQATQ |
OrthoDBi | 444988at2759 |
Enzyme and pathway databases
SignaLinki | V6CLJ5 |
Gene expression databases
Bgeei | WBGene00002245, Expressed in multi-cellular organism and 5 other tissues |
ExpressionAtlasi | V6CLJ5, baseline and differential |
Family and domain databases
Gene3Di | 2.60.40.10, 1 hit 2.60.40.1450, 1 hit |
IDEALi | IID50096 |
InterProi | View protein in InterPro IPR015350, Beta-trefoil_DNA-bd_dom IPR036358, BTD_sf IPR040159, CLS_fam IPR013783, Ig-like_fold IPR014756, Ig_E-set IPR008967, p53-like_TF_DNA-bd IPR015351, RBP-J/Cbf11/Cbf12_DNA-bd IPR037095, RBP-J/Cbf11_DNA-bd_sf IPR038007, RBP-Jkappa_IPT |
PANTHERi | PTHR10665, PTHR10665, 1 hit |
Pfami | View protein in Pfam PF09270, BTD, 1 hit PF09271, LAG1-DNAbind, 1 hit PF20144, TIG_SUH, 1 hit |
SMARTi | View protein in SMART SM01268, BTD, 1 hit SM01267, LAG1_DNAbind, 1 hit |
SUPFAMi | SSF110217, SSF110217, 1 hit SSF49417, SSF49417, 1 hit SSF81296, SSF81296, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | LAG1_CAEEL | |
Accessioni | V6CLJ5Primary (citable) accession number: V6CLJ5 Secondary accession number(s): G5EDU7, Q8MXE7, V6CK60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 7, 2021 |
Last sequence update: | February 19, 2014 | |
Last modified: | May 25, 2022 | |
This is version 56 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families