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Entry version 21 (11 Dec 2019)
Sequence version 1 (19 Feb 2014)
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Protein

Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase

Gene

lhpI

Organism
Azospirillum brasilense
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reduction of both Delta1-pyrroline-2-carboxylate (Pyr2C) and Delta1-piperideine-2-carboxylate (Pip2C) to L-proline and L-pipecolate, respectively, using NADPH or NADH as the electron donor. Can also catalyze the reverse oxidation reactions, albeit at a much lower rate. Together with LhpH, is involved in a trans-3-hydroxy-L-proline (t3LHyp) degradation pathway to L-proline, which allows A.brasilense to grow on t3LHyp as a sole carbon source. Also appears to be involved in D-proline and D-lysine metabolism. Does not show ornithine cyclodeaminase (OCD) activity.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 31400 min(-1) for Pyr2C reduction using NADPH. kcat is 36900 min(-1) for Pyr2C reduction using NADH. kcat is 9950 min(-1) for Pip2C reduction using NADPH. kcat is 9690 min(-1) for Pip2C reduction using NADH. kcat is 621 min(-1) for Delta1-pyrroline-(4S)-hydroxy-2-carboxylate (Pyr4SH2C) reduction using NADPH. kcat is 235 min(-1) for L-proline oxidation using NADP+. kcat is 254 min(-1) for L-proline oxidation using NAD+. kcat is 150 min(-1) for L-pipecolate oxidation using NADP+. kcat is 222 min(-1) for L-pipecolate oxidation using NAD+.1 Publication
  1. KM=0.628 mM for Delta1-pyrroline-2-carboxylate (using NADPH, at pH 6.5)1 Publication
  2. KM=0.837 mM for Delta1-pyrroline-2-carboxylate (using NADH, at pH 6.5)1 Publication
  3. KM=0.474 mM for Delta1-piperideine-2-carboxylate (using NADPH, at pH 6.5)1 Publication
  4. KM=0.600 mM for Delta1-piperideine-2-carboxylate (using NADH, at pH 6.5)1 Publication
  5. KM=0.491 mM for Delta1-pyrroline-(4S)-hydroxy-2-carboxylate (using NADPH, at pH 6.5)1 Publication
  6. KM=3.63 mM for L-proline (using NADP+, at pH 10.5)1 Publication
  7. KM=3.99 mM for L-proline (using NAD+, at pH 10.5)1 Publication
  8. KM=6.54 mM for L-pipecolate (using NADP+, at pH 10.5)1 Publication
  9. KM=14.8 mM for L-pipecolate (using NAD+, at pH 10.5)1 Publication

    pH dependencei

    Optimum pH is 6.5 for Pyr2C reduction and 10.5 for L-proline oxidation.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Amino-acid degradation

    This protein is involved in Amino-acid degradation.1 Publication
    View all proteins of this organism that are known to be involved in Amino-acid degradation.

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionOxidoreductase
    LigandNAD, NADP

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    MetaCyc:MONOMER-18703

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    V5YW53

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductase1 Publication (EC:1.5.1.11 Publication)
    Short name:
    Pyr2C/Pip2C reductase1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:lhpI1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAzospirillum brasilense
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri192 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesRhodospirillaceaeAzospirillum

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    <p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

    Disruption of this gene leads to loss of growth on T3LHyp, D-proline and D-lysine as a sole carbon source, indicating that this gene has dual metabolic functions as a reductase for Pyr2C and Pip2C in these pathways. On the other hand, there is no difference in growth on other carbon sources, including T4LHyp, between the wild-type and mutant strains.

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004322951 – 311Delta(1)-pyrroline-2-carboxylate/Delta(1)-piperideine-2-carboxylate reductaseAdd BLAST311

    <p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

    <p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

    Induced by trans-3-hydroxy-L-proline (T3LHyp), D-proline and D-lysine, but not by trans-4-hydroxy-L-proline (T4LHyp) and by cis-4-hydroxy-D-proline (C4DHyp).1 Publication

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    1 Publication

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    V5YW53

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the ornithine cyclodeaminase/mu-crystallin family.1 Publication

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.30.1780.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR003462 ODC_Mu_crystall
    IPR023401 ODC_N

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR13812 PTHR13812, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF02423 OCD_Mu_crystall, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF001439 CryM, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF51735 SSF51735, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    V5YW53-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MTALSPIPVF DAADTAALLA YPALLATLGQ AVADYAAGEI VSPERLVVPL
    60 70 80 90 100
    QAGGVMLSMP SSARDLATHK LVNVCPGNGA RGLPTILGQV TAYDASTGEM
    110 120 130 140 150
    RFALDGPTVT GRRTAAVTAL GIQALHGAAP RDILLIGTGK QAANHAEALA
    160 170 180 190 200
    AIFPEARLHV RGTSADSAAA FCAAHRAQAP RLVPLDGDAI PDAIDVVVTL
    210 220 230 240 250
    TTSRTPVYRE AAREGRLVVG VGAFTADAAE IDANTVRASR LVVDDPAGAR
    260 270 280 290 300
    HEAGDLIVAQ VDWQHVASLA DVLGGTFDRS GPLLFKSVGC AAWDLAACRT
    310
    ARDALAARRA G
    Length:311
    Mass (Da):31,831
    Last modified:February 19, 2014 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i2059EC3659AA6136
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AB894495 Genomic DNA Translation: BAO21622.1

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB894495 Genomic DNA Translation: BAO21622.1

    3D structure databases

    SMRiV5YW53
    ModBaseiSearch...

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-18703
    SABIO-RKiV5YW53

    Family and domain databases

    Gene3Di3.30.1780.10, 1 hit
    InterProiView protein in InterPro
    IPR036291 NAD(P)-bd_dom_sf
    IPR003462 ODC_Mu_crystall
    IPR023401 ODC_N
    PANTHERiPTHR13812 PTHR13812, 1 hit
    PfamiView protein in Pfam
    PF02423 OCD_Mu_crystall, 1 hit
    PIRSFiPIRSF001439 CryM, 1 hit
    SUPFAMiSSF51735 SSF51735, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPY2CR_AZOBR
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: V5YW53
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: February 19, 2014
    Last modified: December 11, 2019
    This is version 21 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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