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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Acinetobacter brisouii CIP 110357
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Metal bindingi225ManganeseUniRule annotation1
Metal bindingi245Manganese; via tele nitrogenUniRule annotation1
Binding sitei267SubstrateUniRule annotation1
Active sitei269UniRule annotation1
Metal bindingi294ManganeseUniRule annotation1
Binding sitei384GTPUniRule annotation1
Binding sitei415GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi268 – 273GTPUniRule annotation6
Nucleotide bindingi516 – 519GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylaseUniRule annotationSAAS annotation, KinaseImported, Lyase, Transferase
Biological processGluconeogenesisUniRule annotation
LigandGTP-bindingUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding, PyruvateImported

Enzyme and pathway databases

UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Alternative name(s):
GTP-dependent phosphoenolpyruvate carboxykinaseUniRule annotation
Short name:
GTP-PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
ORF Names:P255_01763Imported
OrganismiAcinetobacter brisouii CIP 110357Imported
Taxonomic identifieri1341683 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacter
Proteomesi
  • UP000018418 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 237PEPCK_NInterPro annotationAdd BLAST220
Domaini241 – 603PEPCK_CInterPro annotationAdd BLAST363

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni216 – 218Substrate bindingUniRule annotation3
Regioni382 – 384Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

V2URK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQVNAPEFV RHPKLIAWVE EIAKLTKPAK IEWCDGSEEE YQRLIDLMIA
60 70 80 90 100
NGTMQKLNEE KHPGSYLANS DPSDVARVED RTFICSAKKE DAGATNNWED
110 120 130 140 150
PAVMREKLNG LFDGAMQGRT MYVVPFSMGP LGSHIAHIGI EITDSPYVAV
160 170 180 190 200
SMRKMARMGK AVYDVLGTDG EFVPCVHTVG APLAEGQKDV VWPCNKEKYI
210 220 230 240 250
VHYPETREIW SFGSGYGGNA LLGKKCLALR IASVMGREQG WLAEHMLILG
260 270 280 290 300
ITNPQGEKHY VAAAFPSACG KTNFAMLIPP AGYEGWKIET VGDDIAWIKP
310 320 330 340 350
GEDGRLYAIN PEAGFFGVAP GTNTKTNPNC MATMHKDVIY TNVAVTEDGQ
360 370 380 390 400
VWWEGLSKEV PANLTNWKGQ PHVAGEKAAH PNARFTVSAG QCPSIDADWE
410 420 430 440 450
NPAGVPISAF IFGGRRADTV PLISEAFDWV DGVYKAATMG SETTAAAVGQ
460 470 480 490 500
QGIVRRDPFA MLPFTGYNMA DYFSHWLELG EQVAAKAQAS GNKLPKIFNV
510 520 530 540 550
NWFRRDENGN FVWPGFGQNM RVLEWIINRC ENRANAVETP IGFVPTYDDL
560 570 580 590 600
NWTGTDFSKA DFEKVTSQDK ALWIKEIESH TELFNKLGDR LPKALKARQA
610
ELLEAVKATE TANANV
Length:616
Mass (Da):67,932
Last modified:January 22, 2014 - v1
Checksum:i153B1A6F46882D77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AYEU01000006 Genomic DNA Translation: ESK51255.1
RefSeqiWP_004900251.1, NZ_KI530762.1

Genome annotation databases

EnsemblBacteriaiESK51255; ESK51255; P255_01763
PATRICifig|1341683.3.peg.1752

Similar proteinsi

Entry informationi

Entry nameiV2URK0_9GAMM
AccessioniPrimary (citable) accession number: V2URK0
Entry historyiIntegrated into UniProtKB/TrEMBL: January 22, 2014
Last sequence update: January 22, 2014
Last modified: February 28, 2018
This is version 28 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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