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Protein

ATPase GET3

Gene

GET3

Organism
Microbotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) (Anther smut fungus)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68UniRule annotation1
Binding sitei241ATPUniRule annotation1
Binding sitei268ATPUniRule annotation1
Metal bindingi279Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi282Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 46ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotation
Biological processTransportUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase GET3UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Golgi to ER traffic protein 3UniRule annotation
Guided entry of tail-anchored proteins 3UniRule annotation
Gene namesi
Name:GET3UniRule annotation
ORF Names:MVLG_00298Imported
OrganismiMicrobotryum lychnidis-dioicae (strain p1A1 Lamole / MvSl-1064) (Anther smut fungus)Imported
Taxonomic identifieri683840 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaPucciniomycotinaMicrobotryomycetesMicrobotryalesMicrobotryaceaeMicrobotryum
Proteomesi
  • UP000017200 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation, Endoplasmic reticulumUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 330ArsA_ATPaseInterPro annotationAdd BLAST299

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiEOG092C33XF

Family and domain databases

CDDicd02035 ArsA, 1 hit
HAMAPiMF_03112 Asna1_Get3, 1 hit
InterProiView protein in InterPro
IPR025723 Anion-transp_ATPase-like_dom
IPR016300 ATPase_ArsA/GET3
IPR027542 ATPase_ArsA/GET3_euk
IPR027417 P-loop_NTPase
PANTHERiPTHR10803 PTHR10803, 1 hit
PTHR10803:SF3 PTHR10803:SF3, 1 hit
PfamiView protein in Pfam
PF02374 ArsA_ATPase, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00345 GET3_arsA_TRC40, 1 hit

Sequencei

Sequence statusi: Complete.

U5GYN3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSSQMTYIC AEPEGDALPA NLQNIIDQES LKWIFVGGKG GVGKTTTSCS
60 70 80 90 100
LAIQLAQHRE SVLLISTDPA HNLSDAFSQK FGKEATRVNG FTNLSAMEID
110 120 130 140 150
PSASMQDMVE AGETDSSGMQ GMMQDLAFAI PGIDEAMGFA EVMKHVKSME
160 170 180 190 200
FSVIVFDTAP TGHTLRFLSF PSVLEKALAK LSTLSGRFGP MLNQVSSMMG
210 220 230 240 250
AGVNTGEMFE KLESMRLVVT EVNQQFKNPD LTTFVPVMIS EFLSLYETER
260 270 280 290 300
LIQELTTYQI DVHAIVVNQL LWPDAGSTCK HCNIRWKQQQ KYLKEAYELY
310 320 330
GEDFHIVKMP LLSEEVRGTD SLKQFSNMLI TPYVPTNNK
Length:339
Mass (Da):37,570
Last modified:December 11, 2013 - v1
Checksum:i66206FE8A8104076
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AEIJ01000023 Genomic DNA No translation available.
GL541644 Genomic DNA Translation: KDE09392.1

Genome annotation databases

EnsemblFungiiMVLG_00298T0; MVLG_00298T0; MVLG_00298

Similar proteinsi

Entry informationi

Entry nameiU5GYN3_USTV1
AccessioniPrimary (citable) accession number: U5GYN3
Entry historyiIntegrated into UniProtKB/TrEMBL: December 11, 2013
Last sequence update: December 11, 2013
Last modified: May 23, 2018
This is version 42 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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