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UniProtKB - U3JAV8 (U3JAV8_DANRE)
Protein
CAD protein
Gene
cad
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Catalytic activityi
- EC:3.5.2.3ARBA annotation
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphateARBA annotationEC:6.3.5.5ARBA annotation
- EC:2.1.3.2ARBA annotation
Cofactori
Zn2+ARBA annotation
: UMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1, 2 and 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.ARBA annotation This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.
GO - Molecular functioni
- amino acid binding Source: InterPro
- aspartate carbamoyltransferase activity Source: GO_Central
- ATP binding Source: UniProtKB-UniRule
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-EC
- dihydroorotase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: ZFIN
- 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
- citrulline biosynthetic process Source: GO_Central
- facial nerve morphogenesis Source: ZFIN
- glossopharyngeal nerve morphogenesis Source: ZFIN
- glutamine metabolic process Source: GO_Central
- lymphangiogenesis Source: ZFIN
- neuron projection morphogenesis Source: ZFIN
- nitrogen compound metabolic process Source: GO_Central
- regulation of Notch signaling pathway Source: ZFIN
- regulation of vascular endothelial growth factor receptor signaling pathway Source: ZFIN
- regulation of vascular endothelial growth factor signaling pathway Source: ZFIN
- retina development in camera-type eye Source: ZFIN
- trigeminal nerve morphogenesis Source: ZFIN
- UTP biosynthetic process Source: GO_Central
- vagus nerve morphogenesis Source: ZFIN
Keywordsi
Molecular function | Allosteric enzymeARBA annotation, HydrolaseARBA annotation, LigaseARBA annotation, Multifunctional enzymeARBA annotation, TransferaseARBA annotation |
Biological process | Pyrimidine biosynthesisARBA annotation |
Ligand | ATP-bindingPROSITE-ProRule annotationARBA annotation, Metal-bindingARBA annotation, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-DRE-500753, Pyrimidine biosynthesis |
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:cadImported |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio)Imported |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-021030-4, cad |
Subcellular locationi
Expressioni
Gene expression databases
Bgeei | ENSDARG00000041895, Expressed in paraxial mesoderm and 48 other tissues |
ExpressionAtlasi | U3JAV8, baseline and differential |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 521 – 713 | ATP-graspInterPro annotationAdd BLAST | 193 | |
Domaini | 1057 – 1248 | ATP-graspInterPro annotationAdd BLAST | 192 | |
Domaini | 1313 – 1468 | MGS-likeInterPro annotationAdd BLAST | 156 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1843 – 1877 | DisorderedSequence analysisAdd BLAST | 35 |
Sequence similaritiesi
In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.ARBA annotation
Keywords - Domaini
RepeatARBA annotationPhylogenomic databases
eggNOGi | KOG0370, Eukaryota |
GeneTreei | ENSGT00940000157241 |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51855, MGS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
U3JAV8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLKMASLVL EDGTTFKGRL FGAVSSVSGE VVFQTGMVGY PEALTDPSYK
60 70 80 90 100
SQILTLTYPM IGNYGIPKDE DGEFGLSKWF ESSQIHAAAL IVGEVSENPS
110 120 130 140 150
HWSAAKSLDE WLREQGIPGL QGVDTRCLTK KIREKGTMLG KLVVEGTPAD
160 170 180 190 200
NIPFDNPDAR NLVKEVSMKA PKVFNPEGTV RITAIDCGIK YNQIRCLCQR
210 220 230 240 250
GARVTVVPWD QPLDSNDFDG LFISNGPGNP EYCKETVENI RKVACVENPK
260 270 280 290 300
PIFGICLGHQ LLSLVIGAKT YKMKYGNRGH NQPCIHKGTS RCYITSQNHG
310 320 330 340 350
FAVDPETLPK DWDVLFTNAN DQTSEGIVHN HKPLFSVQFH PEHMAGPTDL
360 370 380 390 400
VGLFDVFLDT VRDVKENKAG KSVKQRLMEH LTFPGSPDPN AFVRPRKVLV
410 420 430 440 450
LGSGGLSIGQ AGEFDYSGSQ AIKAMKEENI QTILINPNIA TVQTSKGLAD
460 470 480 490 500
KVYFLPITPE YVTQVIKNER PDGVLLTFGG QTALNCGVEL KKQGVLEKYK
510 520 530 540 550
VRVLGTPVAS IEMTEDRKIF VEKMEEINEH VAPSEAAMSV EQAVAAAERL
560 570 580 590 600
GYPVLVRSAF ALGGLGSGFA NNRDEMITLV TQAFAHTSQV LVDKSLKGWK
610 620 630 640 650
EIEYEVVRDA YDNCVTVCNM ENIDPLGIHT GESIVVAPSQ TLNDYEYNML
660 670 680 690 700
RNTAIKVIRH LGVVGECNIQ YALNPESEQY YIIEVNARLS RSSALASKAT
710 720 730 740 750
GYPLAYVAAK LSLGIPLPVL KNSVTNSTTA NFEPSLDYCV VKVPRWDLSK
760 770 780 790 800
FLRVSTKIGS SMKSVGEVMA IGRSFEEAFQ KALRMVDENC VGFDHTIKPE
810 820 830 840 850
SEEELQTPTD KRIFVLAAAL HAGYTVERLY ELTKIDHWFL HKMKNIADHK
860 870 880 890 900
KLLETYKQDE SAMPPEIMRK AKQLGFSDKQ IAQAVQSTEL AVRKLRRDWK
910 920 930 940 950
IFPVVKQIDT VAAEWPAQTN YLYLTYHGSE SDVVFEQPHV MVIGSGVYRI
960 970 980 990 1000
GSSVEFDWCA VGCIMELRKM GYKTIMVNYN PETVSTDYDM CDRLYFDEIS
1010 1020 1030 1040 1050
FEVVMDIYEM ENPEGVILSM GGQLPNNIAM SLHRQQCRIL GTSPEFIDNA
1060 1070 1080 1090 1100
ENRFKFSRML DTIGISQPRW KELSDTESAV NFCETVGYPC LVRPSYVLSG
1110 1120 1130 1140 1150
AAMNVAYSDT DMEKFLSSAV AVSKEHPVVI SKFIQEAKEI DVDAVACDGE
1160 1170 1180 1190 1200
VIAIAVSEHV ENAGVHSGDA TLVTPPQDIN QKTMERIKMI VHAIGQELQV
1210 1220 1230 1240 1250
TGPFNLQLIA KDDQLKVIEC NVRVSRSFPY VSKTLGVDLV ALATRVILGE
1260 1270 1280 1290 1300
EVEAVGLMRG NGIVGVKVPQ FSFSRLAGAD VVLGVEMTST GEVACFGENR
1310 1320 1330 1340 1350
YEAYLKAMLS TGFKIPKKNI LLSIGSYKNK SELLPTVQTL ESLGYNLYAS
1360 1370 1380 1390 1400
LGTADFYTEH GVKVMAVDWP FEEESDCPNK DKQRNIMDYL EENHFDLVIN
1410 1420 1430 1440 1450
LSMRNSGGRR LSSFVTKGYR TRRMAIDFSV PLITDIKCTK LFVQALKQIG
1460 1470 1480 1490 1500
QAPRVKTHVD SMTSQKLIRL PGLIDVHVHL REPGATHKED FSSGTAAALA
1510 1520 1530 1540 1550
GGITMVCAMP NTNPAIIDPN SFTMAQKLAK AGCRCDYALF VGASSDNAAL
1560 1570 1580 1590 1600
LPSIASSTAG LKMYLNDTYS TLKMDNVSLW MEHFEKWPKH LPIVAHAEKQ
1610 1620 1630 1640 1650
TVAAILLVAQ LYQRPVHICH VAKKEEILII RAAKQKGIQV TCEVAPHHLF
1660 1670 1680 1690 1700
LCEDNVPVIG KDKAQVRPML GTREDMEALW ENLDIIDCFA TDHAPHSAEE
1710 1720 1730 1740 1750
KISEKPPPGY PGLETMLPLL FTAVSEGRLT VDDIIKRLYE NPRKIFSLPA
1760 1770 1780 1790 1800
QEDTYVEVDL EQEWIIPKHM QFTKSKWTPF EGMKVKGKVM RVVLRGEVAY
1810 1820 1830 1840 1850
IDGQVLVPPG YGQDVKSWSA TPTATTEVIM DAPKRVIEAM TPERPRQAAP
1860 1870 1880 1890 1900
VDVVRSRAPS PRRSAGDGRF ILPPRIHRSS DPGLPPELAL PAEAYAHPPP
1910 1920 1930 1940 1950
LARILSPQAG QPQILPHPQT SPLLHPLVGQ HILSVRQFSK EQMSHLFNVA
1960 1970 1980 1990 2000
HTLRLMVQKE RPLDILKGKV MASMFYEVST RTSSSFAAAM HRLGGSVVHF
2010 2020 2030 2040 2050
CESTSSTQKG ESLVDSVNTM SCYADVIVLR HPIPGAVESA ARHCRRPVIN
2060 2070 2080 2090 2100
AGDGVGEHPT QALLDIFTIR EELGTVNGMT ITMVGDLKHG RTVHSLARLL
2110 2120 2130 2140 2150
TQYRITLRYV APKNLSMPAE IIDFVASKGI KQEEFNSIEE ALPDTDVLYM
2160 2170 2180 2190 2200
TRIQKERFSS EKEYNACFGQ FILTPHIMTG AKRKMVVMHP LPRVNEISVE
2210 2220 2230
VDTDPRAAYF RQAENGMYIR MALLATVLGR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1Q9V4 | F1Q9V4_DANRE | Carbamoyl-phosphate synthetase 2, a... | cad | 485 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX511178 Genomic DNA No translation available. |
RefSeqi | NP_001295754.1, NM_001308825.1 |
Genome annotation databases
Ensembli | ENSDART00000153310.3; ENSDARP00000127377.1; ENSDARG00000041895.10 |
GeneIDi | 266992 |
KEGGi | dre:266992 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BX511178 Genomic DNA No translation available. |
RefSeqi | NP_001295754.1, NM_001308825.1 |
3D structure databases
AlphaFoldDBi | U3JAV8 |
SMRi | U3JAV8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000127377 |
Genome annotation databases
Ensembli | ENSDART00000153310.3; ENSDARP00000127377.1; ENSDARG00000041895.10 |
GeneIDi | 266992 |
KEGGi | dre:266992 |
Organism-specific databases
CTDi | 790 |
ZFINi | ZDB-GENE-021030-4, cad |
Phylogenomic databases
eggNOGi | KOG0370, Eukaryota |
GeneTreei | ENSGT00940000157241 |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Reactomei | R-DRE-500753, Pyrimidine biosynthesis |
Gene expression databases
Bgeei | ENSDARG00000041895, Expressed in paraxial mesoderm and 48 other tissues |
ExpressionAtlasi | U3JAV8, baseline and differential |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51855, MGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | U3JAV8_DANRE | |
Accessioni | U3JAV8Primary (citable) accession number: U3JAV8 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | November 13, 2013 |
Last sequence update: | November 13, 2013 | |
Last modified: | May 25, 2022 | |
This is version 58 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |