Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Entry version 36 (29 Sep 2021)
Sequence version 1 (13 Nov 2013)
Previous versions | rss
Add a publicationFeedback
Protein

Fused isobutyryl-CoA mutase

Gene

icmF

Organism
Geobacillus kaustophilus GBlys
Status
Unreviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible interconversion of isobutyryl-CoA and n-butyryl-CoA, using radical chemistry. Also exhibits GTPase activity, associated with its G-protein domain (MeaI) that functions as a chaperone that assists cofactor delivery and proper holo-enzyme assembly.

UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi23Cobalt (adenosylcobalamin axial ligand)UniRule annotation1
Metal bindingi214Magnesium 1; catalyticUniRule annotation1
Metal bindingi238Magnesium 2; via carbonyl oxygenUniRule annotation1
Metal bindingi239Magnesium 2UniRule annotation1
Metal bindingi252Magnesium 1; catalyticUniRule annotation1
Metal bindingi252Magnesium 2UniRule annotation1
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei255GTPUniRule annotation1
Metal bindingi300Magnesium 1; catalyticUniRule annotation1
Metal bindingi300Magnesium 2UniRule annotation1
Metal bindingi301Magnesium 2; via carbonyl oxygenUniRule annotation1
Binding sitei578Substrate; via carbonyl oxygenUniRule annotation1
Binding sitei613SubstrateUniRule annotation1
Binding sitei719SubstrateUniRule annotation1
Binding sitei763SubstrateUniRule annotation1
Binding sitei812SubstrateUniRule annotation1
Binding sitei847SubstrateUniRule annotation1
Binding sitei852SubstrateUniRule annotation1
Binding sitei964GTPUniRule annotation1
Binding sitei1085GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi210 – 215GTPUniRule annotation6
Nucleotide bindingi347 – 350GTPUniRule annotation4

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChaperoneUniRule annotation, HydrolaseUniRule annotation, IsomeraseUniRule annotationARBA annotation, Multifunctional enzymeUniRule annotation
LigandCobalaminUniRule annotationARBA annotation, Cobalt, GTP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotationARBA annotation, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Fused isobutyryl-CoA mutaseUniRule annotation
Including the following 2 domains:
Isobutyryl-CoA mutaseUniRule annotation (EC:5.4.99.13UniRule annotation)
Short name:
ICMUniRule annotation
P-loop GTPaseUniRule annotation (EC:3.6.5.-UniRule annotation)
Alternative name(s):
G-protein chaperoneUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:icmFUniRule annotation
ORF Names:GBL_1103Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiGeobacillus kaustophilus GBlysImported
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1337888 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000016424 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Unassembled WGS sequence

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

UniRule annotation

GO - Molecular functioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini10 – 140B12-bindingInterPro annotationAdd BLAST131

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and domains' section denotes the positions of regions of coiled coil within the protein.<p><a href='/help/coiled' target='_top'>More...</a></p>Coiled coili179 – 199Sequence analysisAdd BLAST21
Coiled coili475 – 495Sequence analysisAdd BLAST21

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Is composed of four functional domains: the N-terminal 5'-deoxyadenosylcobalamin binding region that is homologous to the small subunit of ICM (IcmB), a middle P-loop GTPase domain (MeaI) that likely acts as a chaperone for ICM, a structured linker region involved in dimer formation, and a C-terminal part that is homologous to the large substrate-binding subunit of ICM (IcmA).UniRule annotation

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the IcmF family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.300, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_02050, IcmF, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR006159, Acid_CoA_mut_C
IPR016176, Cbl-dep_enz_cat
IPR006158, Cobalamin-bd
IPR036724, Cobalamin-bd_sf
IPR033669, IcmF
IPR006099, MeMalonylCoA_mutase_a/b_cat
IPR027417, P-loop_NTPase

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02310, B12-binding, 1 hit
PF01642, MM_CoA_mutase, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51703, SSF51703, 1 hit
SSF52242, SSF52242, 1 hit
SSF52540, SSF52540, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00640, acid_CoA_mut_C, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51332, B12_BINDING, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

U2WQ53-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAHIYRPKHH VRFVTASSLF DGHDASINIM RRILQASGAE VIHLGHNRSV
60 70 80 90 100
EEIVNAAIQE DVQGIAVSSY QGGHMEFFKY MYDLLQERGA SHIRIYGGGG
110 120 130 140 150
GVIIPREIKE LHEYGIARIF SPEDGRRLGL QGMINVMLEE CDFPTVTTVT
160 170 180 190 200
DELERLPSGD VQAIARLITL CEYRAEGENR EAAAAAEAAI EQVKALEKRV
210 220 230 240 250
PVLGITGTGG AGKSSLTDEL VRRFLNEIPD IKIAILSVDP TKQKTGGALL
260 270 280 290 300
GDRIRMNSIN SPRVYMRSLA TRHSRTELSP AIRDAISVVK AAGFDLVIIE
310 320 330 340 350
TSGIGQGDAA ITEVCDVSMY VMTSEFGAPT QLEKIDMIDY ADLIVINKFE
360 370 380 390 400
RKGSEDAKRQ VQKQYQRSHQ LFDRDVSEMP VYGTIASQFN DPGTNTLFVA
410 420 430 440 450
LVDTINKKAG TNWKTSLKTV ANVEKHNVII PNERRYYLRE IAETVRSYHR
460 470 480 490 500
RAEQQVEVAR RLFQIEGAIE AAKERGEAED VIRALETLKA DYEAKLTPES
510 520 530 540 550
KRILATWEET KAKYAAKQFV TKVRDKEIVT ELTTKTLSGL DIPKVVLPKF
560 570 580 590 600
KDYGEILRWV YKENVPGSFP YTAGVFPFKR QGEDPKRQFA GEGTPERTNR
610 620 630 640 650
RFHYLCKEDK AKRLSTAFDS VTLYGEDPDY RPDIFGKVGE SGVSVCTLDD
660 670 680 690 700
MKKLYKGFDL CDPLTSVSMT INGPAPILLA MFMNTAIDQQ VEKKEAELGR
710 720 730 740 750
PLTPEEYEQV KEWTLQTVRG TVQADILKED QGQNTCIFST DFALKMMGDI
760 770 780 790 800
QEYFIKHRVR NYYSVSISGY HIAEAGANPI TQLAFTLANG FTYVEYYLSR
810 820 830 840 850
GMHIDDFAPN LSFFFSNGLD PEYSVIGRVA RRIWAIVMRE KYGANERSQK
860 870 880 890 900
LKYHIQTSGR SLHAQEIDFN DIRTTLQALL AIYDNCNSLH TNAYDEAITT
910 920 930 940 950
PTEESVRRAM AIQLIITKEF GLTKNENPLQ GSFIIEELTD LVEEAVLQEF
960 970 980 990 1000
ERLNDRGGVL GAMEMQYQRG KIQDESLYYE TKKHTGELPI IGVNTFLNPN
1010 1020 1030 1040 1050
PPSEDELNNI QLARATYEEK ETQIRNLREF QERNKDKAGP ALERLKQVAT
1060 1070 1080
SGGNIFEELM ETVKVASLGQ ITRALYEVGG QYRRNM
Length:1,086
Mass (Da):122,459
Last modified:November 13, 2013 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5FCB6448771EEBE4
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BASG01000006 Genomic DNA Translation: GAD12886.1

NCBI Reference Sequences

More...
RefSeqi
WP_014196968.1, NZ_BASG01000006.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
GAD12886; GAD12886; GBL_1103

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BASG01000006 Genomic DNA Translation: GAD12886.1
RefSeqiWP_014196968.1, NZ_BASG01000006.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

EnsemblBacteriaiGAD12886; GAD12886; GBL_1103

Family and domain databases

Gene3Di3.40.50.300, 1 hit
HAMAPiMF_02050, IcmF, 1 hit
InterProiView protein in InterPro
IPR006159, Acid_CoA_mut_C
IPR016176, Cbl-dep_enz_cat
IPR006158, Cobalamin-bd
IPR036724, Cobalamin-bd_sf
IPR033669, IcmF
IPR006099, MeMalonylCoA_mutase_a/b_cat
IPR027417, P-loop_NTPase
PfamiView protein in Pfam
PF02310, B12-binding, 1 hit
PF01642, MM_CoA_mutase, 1 hit
SUPFAMiSSF51703, SSF51703, 1 hit
SSF52242, SSF52242, 1 hit
SSF52540, SSF52540, 1 hit
TIGRFAMsiTIGR00640, acid_CoA_mut_C, 1 hit
PROSITEiView protein in PROSITE
PS51332, B12_BINDING, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiU2WQ53_GEOKU
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: U2WQ53
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: September 29, 2021
This is version 36 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again