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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus pyogenes GA06023
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162PROSITE-ProRule annotation1
Active sitei162NucleophileUniRule annotation1
Binding sitei1895,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei197dUMPUniRule annotation1
Binding sitei2865,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi141 – 142dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi186 – 189dUMPUniRule annotation4
Nucleotide bindingi227 – 229dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotationImported
ORF Names:HMPREF1231_1866Imported
OrganismiStreptococcus pyogenes GA06023Imported
Taxonomic identifieri1115816 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000016663 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 286Thymidylat_syntInterPro annotationAdd BLAST271

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

U2TY56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTERLLEMT KADQIFKANI QKIINEGSLS EQARPKYKDG RTAHSKYITG
60 70 80 90 100
AFAEYDLAKG EFPITTLRPI PIKSAIKELF WIYQDQSNSL DVLEAKYNVH
110 120 130 140 150
YWNEWEVDQT RTIGQRYGAV VKKHDIISKI LKQLAENPWN RRNVISLWDY
160 170 180 190 200
EAFEETKGLL PCAFQIMFDV RRVGEDLYLD ASLTQRSNDI LVAHHINAMQ
210 220 230 240 250
YVALQMMIAK HFGWKIGKFF YFVNNLHIYD NQFDQAQELL KRQPVASQPK
260 270 280
LVLNVPDRTN FFDIKPDDFE LQNYDPVKPQ LHFDLAI
Length:287
Mass (Da):33,650
Last modified:November 13, 2013 - v1
Checksum:i78A239F4A92B7B88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AWPJ01000335 Genomic DNA Translation: ERL10953.1

Genome annotation databases

EnsemblBacteriaiERL10953; ERL10953; HMPREF1231_1866
PATRICifig|1115816.3.peg.1544

Similar proteinsi

Entry informationi

Entry nameiU2TY56_STRPY
AccessioniPrimary (citable) accession number: U2TY56
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: February 28, 2018
This is version 30 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
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Main funding by: National Institutes of Health