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Protein

Probable dual-specificity RNA methyltransferase RlmN

Gene

rlmN

Organism
Actinomyces sp. oral taxon 877 str. F0543
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs.SAAS annotation

Miscellaneous

Reaction proceeds by a ping-pong mechanism involving intermediate methylation of a conserved cysteine residue.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation
2 S-adenosyl-L-methionine + adenine(37) in tRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(37) in tRNA + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei140Proton acceptorUniRule annotation1
Metal bindingi160Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi164Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi167Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation1
Binding sitei249S-adenosyl-L-methionineUniRule annotation1
Binding sitei351S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei394S-methylcysteine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotationImported, Transferase
Biological processrRNA processingUniRule annotationSAAS annotation, tRNA processingUniRule annotationSAAS annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Probable dual-specificity RNA methyltransferase RlmNUniRule annotation (EC:2.1.1.192UniRule annotation)
Alternative name(s):
23S rRNA (adenine(2503)-C(2))-methyltransferaseUniRule annotation
23S rRNA m2A2503 methyltransferaseUniRule annotation
Ribosomal RNA large subunit methyltransferase NUniRule annotation
tRNA (adenine(37)-C(2))-methyltransferaseUniRule annotation
tRNA m2A37 methyltransferaseUniRule annotation
Gene namesi
Name:rlmNUniRule annotation
ORF Names:HMPREF1550_00640Imported
OrganismiActinomyces sp. oral taxon 877 str. F0543Imported
Taxonomic identifieri1227264 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinomycetalesActinomycetaceaeActinomyces
Proteomesi
  • UP000016532 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Keywords - PTMi

Disulfide bondUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliU1R138
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini155 – 324Radical_SAMInterPro annotationAdd BLAST170

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 216S-adenosyl-L-methionine bindingUniRule annotation2
Regioni272 – 274S-adenosyl-L-methionine bindingUniRule annotation3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili318 – 338Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the radical SAM superfamily. RlmN family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

OrthoDBiPOG091H02LT

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01849 RNA_methyltr_RlmN, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR027492 RNA_MTrfase_RlmN
IPR004383 rRNA_lsu_MTrfase_RlmN/Cfr
IPR007197 rSAM
PANTHERiPTHR30544 PTHR30544, 1 hit
PfamiView protein in Pfam
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF006004 CHP00048, 1 hit
SFLDiSFLDF00275 adenosine_C2_methyltransferase, 1 hit
SFLDS00029 Radical_SAM, 1 hit
TIGRFAMsiTIGR00048 rRNA_mod_RlmN, 1 hit

Sequencei

Sequence statusi: Complete.

U1R138-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTRTQVAAR GAAAGAGREV RPTDQPPEGA AHAGARPVLS FTAKRRGKPP
60 70 80 90 100
SHLADLDEAG RKAVLASMGL PPFRADQLSR HYFERFEADP ADMSDIPASM
110 120 130 140 150
RQRVRDSLLP PLVSGVVSLR ADGGRTVKDL WRLYDGAQVE SVLMRYPQRT
160 170 180 190 200
TLCVSSQAGC GMACPFCATG QMGLTRNLST AEIVDQVRLA QAACRDGALA
210 220 230 240 250
GGPTRLTNVV FMGMGEPLAN YKTVVGALHR LVDPVPEGFG MSARNVTVST
260 270 280 290 300
VGLVPAIRRL AGEGLPVTLA VSLHAPDDDL RDDLIPVNSR WKVGELLDAA
310 320 330 340 350
RHYFLVTGRR VSIEYALIKD MNDQVWRAQL LADELNRRGK GWAHVNPIPL
360 370 380 390 400
NPTPGSIWTA STRRSQDAFV ATLRDNGVVT SIRDTRGSDI DGACGQLATS
410
AAKRAAGDGS SRKETR
Length:416
Mass (Da):44,635
Last modified:November 13, 2013 - v1
Checksum:i9BBF750B44A983A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AWSG01000027 Genomic DNA Translation: ERH33295.1

Genome annotation databases

EnsemblBacteriaiERH33295; ERH33295; HMPREF1550_00640
PATRICifig|1227264.3.peg.554

Similar proteinsi

Entry informationi

Entry nameiU1R138_9ACTO
AccessioniPrimary (citable) accession number: U1R138
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: December 20, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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