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Protein

Multifunctional fusion protein

Gene

carB

Organism
Treponema socranskii subsp. socranskii VPI DR56BR1116 = ATCC 35536
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotationSAAS annotation
LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Cofactori

Mn2+SAAS annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional fusion protein (carB)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional fusion protein (carB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.UniRule annotationSAAS annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Multifunctional fusion protein (carB)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (HMPREF1325_1521)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1112SubstrateUniRule annotation1
Binding sitei1163SubstrateUniRule annotation1
Sitei1287Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Binding sitei1318SubstrateUniRule annotation1
Sitei1336Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation1
Active sitei1345Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseUniRule annotationImported, LigaseUniRule annotationImported
Biological processAmino-acid biosynthesis, Arginine biosynthesisUniRule annotation, Lysine biosynthesisUniRule annotation, Pyrimidine biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotation, ManganeseSAAS annotation, Metal-bindingSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00034;UER00025

UPA00068;UER00171

UPA00070;UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional fusion proteinUniRule annotation
Including the following 2 domains:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Alternative name(s):
PLP-independent amino acid racemaseUniRule annotation
Carbamoyl-phosphate synthase large chainUniRule annotation (EC:6.3.5.5UniRule annotation)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chainUniRule annotation
Gene namesi
Name:carBUniRule annotation
Synonyms:dapFUniRule annotation
ORF Names:HMPREF1325_0913Imported
OrganismiTreponema socranskii subsp. socranskii VPI DR56BR1116 = ATCC 35536Imported
Taxonomic identifieri1125725 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000016412 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.UniRule annotationSAAS annotation
Homodimer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini133 – 328ATP-graspInterPro annotationAdd BLAST196
Domaini700 – 890ATP-graspInterPro annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 402Carboxyphosphate synthetic domainUniRule annotationAdd BLAST402
Regioni959 – 1402Allosteric domainUniRule annotationAdd BLAST444
Regioni1173 – 1174Substrate bindingUniRule annotation2
Regioni1336 – 1337Substrate bindingUniRule annotation2
Regioni1346 – 1347Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the CarB family.UniRule annotationSAAS annotation
Belongs to the diaminopimelate epimerase family.UniRule annotation

Keywords - Domaini

RepeatUniRule annotation

Phylogenomic databases

OrthoDBiPOG091H01IP

Family and domain databases

Gene3Di1.10.1030.10, 1 hit
3.40.50.1380, 1 hit
HAMAPiMF_01210_B CPSase_L_chain_B, 1 hit
MF_00197 DAP_epimerase, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR001653 DAP_epimerase_DapF
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PF01678 DAP_epimerase, 2 hits
PF02142 MGS, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52335 SSF52335, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
TIGR00652 DapF, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00867 CPSASE_2, 2 hits

Sequencei

Sequence statusi: Complete.

U1GRY7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPLNTSIRKV MVIGSGPIVI GQAVEFDYAG TQACKALKEQ GLSVVLVNSN
60 70 80 90 100
PATLMTDRSM ADAVYIEPLI PETIRRIIEK EKPDSLLSTL GGQTGLTLSM
110 120 130 140 150
ELAKSGFLKS HGVQLLGARP DTIDKAEDRQ IFKDTMLSIG EPCIPSKVVT
160 170 180 190 200
TLEDAVDFAE HEIGYPAIVR PAFTLGGTGG GIAETKSELE EIAQNGLHRS
210 220 230 240 250
PIHQILVEKC IAGWKEIEFE VVRDHSGNAI TVCSMENVDP VGIHTGDSIV
260 270 280 290 300
VAPTMTLSDK EYQMLRSASF NIVGSLGMEG GCNVQFALHP STSEYAVIEV
310 320 330 340 350
NPRVSRSSAL ASKATGYPIA RVATLIAIGY NLDEIPNFVT KKTSAYFEPV
360 370 380 390 400
VDYVVVKMPK FPFDKFVYAK RTLGTQMKAT GEVMSIGRTF EEAIMKAVRG
410 420 430 440 450
AEIGTDSMRL KQFVNESDER IKERIGECTD RRIFAIYEAL VRGIMSIDEI
460 470 480 490 500
NKITKIDIWF LAKLQHIAFR ELELASVKTG KQKLSPLLYL EAKKDGFPDK
510 520 530 540 550
TITELSGVAI PGGSGIIKEA KDAAALVKQG KLAHIPATYK MVDTCTGEFS
560 570 580 590 600
AETPYFYGGY YEENEAADFL AEHAKSKSKK GTIIVLGSGP IRIGQGIEFD
610 620 630 640 650
YASVQCVWSL KKLGYDVVTI NNNPETVSTD FDTSDRLYFE PLTPEDVMSV
660 670 680 690 700
INTEKPIGVV VAFGGQTAIK LTKFLDEQGI KILGTSAESI DIAEDREKFE
710 720 730 740 750
ALCVKLGINK PAGETVLTTE EAIASAQRIG YPILLRPSYV LGGQNMIIAW
760 770 780 790 800
GDADVREYMG IILSQGIENP VLIDKYMGGT ELEVDAICDG KDILIPGIME
810 820 830 840 850
HIERAGIHSG DSIAVYPSWN LNDILRQKII KQSRELALAL GTKGLVNIQY
860 870 880 890 900
LIYNNDLYVI EVNPRSSRTV PYISKVTGVP MVELAVRAML GEKVKTFGYG
910 920 930 940 950
TGLYRISPYF AVKVPVFSFE KLMDVDTHLG PEMKSTGEVL GLASTMEEAI
960 970 980 990 1000
YKGLIGAGYR MKRSGGVLFT VRKTDQFEIP DLARKFYDMG FTLYATEGTA
1010 1020 1030 1040 1050
GVIKDFGMEV TVVNKIHENP DDNLLTLLDT GKIDYVISTS TKGRDPHADS
1060 1070 1080 1090 1100
VRMRRHAVER DIPCLTAIDT ANAIANCLMS HYNAENVELV DINALRESKE
1110 1120 1130 1140 1150
KLRFCKMQST GNDFILIDAR KQAVANPAGL AVRLCNRRME IGADSLVLVK
1160 1170 1180 1190 1200
DSDKADAYMQ FFNQDGSEGR LAGNAIRSVA KYLYDNNING VKDRGDAASP
1210 1220 1230 1240 1250
TASLSIDTAS GTKSLVLYKL DGKVSSVTVD MGRPLFDAAS LPTTLSPVST
1260 1270 1280 1290 1300
SRESLAARIP QKAIVNVPLT VDGTKYDVTC LSVGTPHCVV FCGFVDKVDV
1310 1320 1330 1340 1350
EKIGPLFENN AVFPNRTNTE FVRVVGRNEL KMRTWERGNG ETPACGTGAC
1360 1370 1380 1390 1400
AAAIAAVLNG YCPMDENITV QVKGGTLIVK YTGDTVYLTG QSDTVYEGEI

EI
Length:1,402
Mass (Da):152,859
Last modified:November 13, 2013 - v1
Checksum:i87141E64A78732FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AUZJ01000071 Genomic DNA Translation: ERF59429.1

Genome annotation databases

EnsemblBacteriaiERF59429; ERF59429; HMPREF1325_0913
PATRICifig|1125725.3.peg.2665

Similar proteinsi

Entry informationi

Entry nameiU1GRY7_TRESO
AccessioniPrimary (citable) accession number: U1GRY7
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: July 18, 2018
This is version 38 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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