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Protein

tRNA(Ile)-lysidine synthase

Gene

tilS

Organism
Bacillus licheniformis CG-B52
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.UniRule annotationSAAS annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

(tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H2O.UniRule annotationSAAS annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi25 – 30ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationSAAS annotation
Biological processtRNA processingUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(Ile)-lysidine synthaseUniRule annotationSAAS annotation (EC:6.3.4.19UniRule annotationSAAS annotation)
Alternative name(s):
tRNA(Ile)-2-lysyl-cytidine synthaseUniRule annotation
tRNA(Ile)-lysidine synthetaseUniRule annotation
Gene namesi
Name:tilSUniRule annotation
ORF Names:N399_00390Imported
OrganismiBacillus licheniformis CG-B52Imported
Taxonomic identifieri1368424 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000017025 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini385 – 458TilS_CInterPro annotationAdd BLAST74

Domaini

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.UniRule annotation

Sequence similaritiesi

Belongs to the tRNA(Ile)-lysidine synthase family.UniRule annotationSAAS annotation

Family and domain databases

CDDicd01992 PP-ATPase, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_01161 tRNA_Ile_lys_synt, 1 hit
InterProiView protein in InterPro
IPR012796 Lysidine-tRNA-synth_C
IPR014729 Rossmann-like_a/b/a_fold
IPR011063 TilS/TtcA_N
IPR012094 tRNA_Ile_lys_synt
IPR012795 tRNA_Ile_lys_synt_N
IPR015262 tRNA_Ile_lys_synt_subst-bd
PANTHERiPTHR43033:SF1 PTHR43033:SF1, 1 hit
PfamiView protein in Pfam
PF01171 ATP_bind_3, 1 hit
PF09179 TilS, 1 hit
PF11734 TilS_C, 1 hit
SMARTiView protein in SMART
SM00977 TilS_C, 1 hit
TIGRFAMsiTIGR02433 lysidine_TilS_C, 1 hit
TIGR02432 lysidine_TilS_N, 1 hit

Sequencei

Sequence statusi: Complete.

T5HW33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSIEDFMKK YELSFDGVTL IAGVSGGPDS MALLHALHHT VPASATLIAA
60 70 80 90 100
HVDHMFRGEE SERDMRFVQD YCEAEGIQCE AVQIDVLAFA AENNLNKQAA
110 120 130 140 150
ARECRYAFFQ ELMKRHRAEY LVLGHHGDDQ VETMLMKMAK GTVGIGLAGI
160 170 180 190 200
QPKRRFDSGW LLRPFLRLSK DDLLAYCKEN RIPFRTDPSN SEDDYTRNRF
210 220 230 240 250
RHHVLPFLKK ESEDVHKRFQ SVSEFLTEDE LYLQALTKDK MNTVITSKSS
260 270 280 290 300
SGVEISIERL LALPMPLQRR GVHLILNYLY ENVPSAFSAH HIQVFLDWIS
310 320 330 340 350
QDGPSGSLDF PNGLKVAKSY HTCLFTFQRL QCENVSYHYE ISGAGEEELI
360 370 380 390 400
LPGGSSLHVS GPGSTRKLQG NDVFATSPKQ VQFPLYVRTR QNGDRVKLKG
410 420 430 440 450
MNGSKKVKDI FIDEKVPLSK RDSWPIVTDA VGNIIWIPGL KKTIFEELDV
460 470
TNNDRIVLQY RQHEKCRGLA KNETGY
Length:476
Mass (Da):54,014
Last modified:November 13, 2013 - v1
Checksum:i6FFE55C19E785FBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AVEZ01000023 Genomic DNA Translation: EQM28527.1
RefSeqiWP_016886275.1, NZ_AVEZ01000023.1

Genome annotation databases

EnsemblBacteriaiEQM28527; EQM28527; N399_00390
PATRICifig|1368424.3.peg.1128

Similar proteinsi

Entry informationi

Entry nameiT5HW33_BACLI
AccessioniPrimary (citable) accession number: T5HW33
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: February 28, 2018
This is version 27 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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