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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Clostridioides difficile CD160
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferaseUniRule annotationImported, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotationImported
ORF Names:QEW_0105Imported
OrganismiClostridioides difficile CD160Imported
Taxonomic identifieri1151292 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesPeptostreptococcaceaeClostridioides
Proteomesi
  • UP000015838 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymeraseUniRule annotationImported

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliT3DPH3
SMRiT3DPH3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 227RPOLDInterPro annotationAdd BLAST208

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 228Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST228
Regioni247 – 315Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST69

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

OrthoDBiPOG091H001Z

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

T3DPH3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIEKPKVD IVELSEDYRY GKFVIEPLER GYGITIGNAL RRILLSSLPG
60 70 80 90 100
VAVNAIKIDG VLHEFSTIPG VKEDVTEIIL TLKELSATID GEGSRTLKIE
110 120 130 140 150
AQGPCSITGA DIICPPDVEI LSKDLAIATL DDNAKLNMEI FVDKGRGYVS
160 170 180 190 200
AEENKTENVP IGVLPVDSIY TPVEKVSYHV ENTRVGQKTD YDKLVLEVWT
210 220 230 240 250
NGSINPQEGI SLAAKVLVEH LNLFIDLTEH VSSVEIMVEK EEDQKEKVLE
260 270 280 290 300
MTIEELDLSV RSYNCLKRAG INTVEELANK SEDDMMKVRN LGKKSLEEVI
310
QKLEELGLGL KPSEE
Length:315
Mass (Da):34,920
Last modified:November 13, 2013 - v1
Checksum:iE75526C3C02D9B16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AVHW01000015 Genomic DNA Translation: EQF29438.1
RefSeqiWP_003427733.1, NZ_AVHW01000015.1

Genome annotation databases

EnsemblBacteriaiEQF29438; EQF29438; QEW_0105
GeneIDi31351592
PATRICifig|1151292.4.peg.326

Similar proteinsi

Entry informationi

Entry nameiT3DPH3_CLODI
AccessioniPrimary (citable) accession number: T3DPH3
Entry historyiIntegrated into UniProtKB/TrEMBL: November 13, 2013
Last sequence update: November 13, 2013
Last modified: April 25, 2018
This is version 26 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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