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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Chlamydia psittaci C1/97
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei595ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Metal-bindingUniRule annotation, Nucleotide-binding, ZincUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotationImported
ORF Names:CPC197_0828Imported
OrganismiChlamydia psittaci C1/97Imported
Taxonomic identifieri1112248 [NCBI]
Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
Proteomesi
  • UP000014856 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 629tRNA-synt_1InterPro annotationAdd BLAST611
Domaini680 – 826Anticodon_1InterPro annotationAdd BLAST147

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi49 – 59"HIGH" regionUniRule annotationAdd BLAST11
Motifi592 – 596"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

S7KA36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTEGEGSRE SLANREERIL NFWKTQEIFQ KSLKNREGRT LYSFYDGPPF
60 70 80 90 100
ATGLPHYGHL LAGTIKDVVG RFATMDGYYV PRRFGWDCHG VPVEYEVEKS
110 120 130 140 150
LNLTTPGAIE DFGVARFNEE CRKIVFRYVD EWEHYINRVG RWVDFSATWK
160 170 180 190 200
TMDASFMESV WWVFRSLYDQ GLVYEGVKVV PFSTKLGTPL SNFEAGQNYK
210 220 230 240 250
EVDDPSVVIK FALHGDSASL LVWTTTPWTL VSNMAVAVNP EITYVRVADK
260 270 280 290 300
VSGEQWILGQ GCLSRWFSDP DTYEVIESFP GTTLIGKSYE PPFNFFEQKR
310 320 330 340 350
AEGAYKILPG SFVEESEGTG VVHMAPAFGE ADFFVCKEHR VPMVCPVDNH
360 370 380 390 400
GCFTEEIPEY QGQYIKSCDK GIIKSLKTQG KVFYHGTIMH RYPFCWRTDT
410 420 430 440 450
PLIYKTVNSW FVSVEKIKDK MLQANKKIHW VPEHIKAGRF GKWLEGARDW
460 470 480 490 500
AISRNRYWGT PIPVWKSKDG EILVIGSVEE LEKLTGEKVS DLHCHFVDQL
510 520 530 540 550
KVEKDGKSFH RVPYVFDCWF DSGAMPYAQN HYPFENQEAT ESGFPADFIA
560 570 580 590 600
EGLDQTRGWF YTLTVISAAL FDQPAFKNAI VNGIVLAEDG NKMSKRLNNY
610 620 630 640 650
PSPMSIMNTY GADALRLYLL DSVVVKAEDL RFSDKGVESV LKQVLLPLTN
660 670 680 690 700
VLSFFKTYTD LYGFDANNYD KEEISYSEID KWILSNLYTV VGKVRESMSS
710 720 730 740 750
YNLNTAVNPF VTFIDDLTNW YIRRCRRRFW ESADTPDRRA AFATLYEVLT
760 770 780 790 800
VFCRVIAPFI PFISEDIYQQ IKTENSLESV HLCDFPHVDL AKVFPDLEQR
810 820 830 840 850
MGDAREIVGL GHSLRKEHKL KVRQPLANFY VVGPKDRLDE LASFEQLIAE
860 870 880 890 900
ELNVKNIVFY KEAPSFVKTT VKPNFRSLGR RVGEKIKDIQ KALASLSQEQ
910 920 930 940 950
IQLLLKQEYI SLNLGSEELI LNMDDVLISW ETDPGYVARS SSLFTVVLDC
960 970 980 990 1000
QLTEELIVEA ISRELVNKIN TMRRNQKLHV SDRIVLRIQT SEDVRKAFVH
1010 1020 1030 1040
YVDYICEETL TTQWEFVDIL EGEEWDINGH PTVIAIEVAA RPH
Length:1,043
Mass (Da):119,833
Last modified:October 16, 2013 - v1
Checksum:iCD27EB3ADF8A010A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
ATMZ01000170 Genomic DNA Translation: EPP31420.1

Genome annotation databases

EnsemblBacteriaiEPP31420; EPP31420; CPC197_0828
PATRICifig|1112248.4.peg.696

Similar proteinsi

Entry informationi

Entry nameiS7KA36_CHLPS
AccessioniPrimary (citable) accession number: S7KA36
Entry historyiIntegrated into UniProtKB/TrEMBL: October 16, 2013
Last sequence update: October 16, 2013
Last modified: March 28, 2018
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

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