UniProtKB - S4R255 (S4R255_MOUSE)
Protein
Nitric oxide synthase
Gene
Nos1
Organism
Mus musculus (Mouse)
Status
Functioni
Produces nitric oxide (NO).UniRule annotation
Catalytic activityi
- H+ + 2 L-arginine + 3 NADPH + 4 O2 = 4 H2O + 2 L-citrulline + 3 NADP+ + 2 nitric oxideUniRule annotationARBA annotationEC:1.14.13.39UniRule annotationARBA annotation
Cofactori
Protein has several cofactor binding sites:- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterinARBA annotation
- hemeUniRule annotationARBA annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 415 | Iron (heme axial ligand)UniRule annotation | 1 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: InterPro
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- nitric-oxide synthase activity Source: UniProtKB-EC
GO - Biological processi
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | Calmodulin-bindingUniRule annotationARBA annotation, OxidoreductaseUniRule annotationARBA annotation |
Ligand | FADUniRule annotationARBA annotation, Flavoprotein, FMNUniRule annotationARBA annotation, HemeUniRule annotationARBA annotation, Iron, Metal-binding, NADPUniRule annotationARBA annotation |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Nos1Imported |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97360, Nos1 |
Subcellular locationi
Plasma membrane
- sarcolemma ARBA annotation; Peripheral membrane protein ARBA annotation
Other locations
- dendritic spine ARBA annotation
Plasma Membrane
- sarcolemma Source: UniProtKB-SubCell
Other locations
- dendritic spine Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projectionARBA annotationPTM / Processingi
Keywords - PTMi
Ubl conjugationARBA annotationProteomic databases
PRIDEi | S4R255 |
PTM databases
SwissPalmi | S4R255 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029361, Expressed in anterior amygdaloid area and 200 other tissues |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 99 | PDZInterPro annotationAdd BLAST | 83 | |
Domaini | 755 – 935 | Flavodoxin-likeInterPro annotationAdd BLAST | 181 | |
Domaini | 990 – 1237 | FAD-binding FR-typeInterPro annotationAdd BLAST | 248 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 114 – 174 | DisorderedSequence analysisAdd BLAST | 61 | |
Regioni | 271 – 298 | DisorderedSequence analysisAdd BLAST | 28 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 114 – 143 | PolarSequence analysisAdd BLAST | 30 | |
Compositional biasi | 160 – 174 | PolarSequence analysisAdd BLAST | 15 | |
Compositional biasi | 271 – 294 | PolarSequence analysisAdd BLAST | 24 |
Sequence similaritiesi
Phylogenomic databases
GeneTreei | ENSGT00940000159357 |
HOGENOMi | CLU_001570_16_0_1 |
OMAi | ERGHFYV |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
S4R255-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEEHTFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG
60 70 80 90 100
AAEQSGLIQA GDIILAVNDR PLVDLSYDSA LEVLRGIASE THVVLILRGP
110 120 130 140 150
EGFTTHLETT FTGDGTPKTI RVTQPLGTPT KAVDLSRQPS ASKDQPLAVD
160 170 180 190 200
RVPGPSNGPQ HAQGRGQGAG SVSQANGVAI DPTMKNTKAN LQDSGEQDEL
210 220 230 240 250
LKEIEPVLSI LTGGGKAVNR GGPAKAEMKD TGIQVDRDLD GKLHKAPPLG
260 270 280 290 300
GENDRVFNDL WGKGNVPVVL NNPYSENEQS PASGKQSPTK NGSPSRCPRF
310 320 330 340 350
LKVKNWETDV VLTDTLHLKS TLETGCTEQI CMGSIMLPSH HIRKSEDVRT
360 370 380 390 400
KDQLFPLAKE FLDQYYSSIK RFGSKAHMDR LEEVNKEIES TSTYQLKDTE
410 420 430 440 450
LIYGAKHAWR NASRCVGRIQ WSKLQVFDAR DCTTAHGMFN YICNHVKYAT
460 470 480 490 500
NKGNLRSAIT IFPQRTDGKH DFRVWNSQLI RYAGYKQPDG STLGDPANVE
510 520 530 540 550
FTEICIQQGW KPPRGRFDVL PLLLQANGND PELFQIPPEL VLEVPIRHPK
560 570 580 590 600
FDWFKDLGLK WYGLPAVSNM LLEIGGLEFS ACPFSGWYMG TEIGVRDYCD
610 620 630 640 650
NSRYNILEEV AKKMDLDMRK TSSLWKDQAL VEINIAVLYS FQSDKVTIVD
660 670 680 690 700
HHSATESFIK HMENEYRCRG GCPADWVWIV PPMSGSITPV FHQEMLNYRL
710 720 730 740 750
TPSFEYQPDP WNTHVWKGTN GTPTKRRAIG FKKLAEAVKF SAKLMGQAMA
760 770 780 790 800
KRVKATILYA TETGKSQAYA KTLCEIFKHA FDAKAMSMEE YDIVHLEHEA
810 820 830 840 850
LVLVVTSTFG NGDPPENGEK FGCALMEMRH PNSVQEERKS YKVRFNSVSS
860 870 880 890 900
YSDSRKSSGD GPDLRDNFES TGPLANVRFS VFGLGSRAYP HFCAFGHAVD
910 920 930 940 950
TLLEELGGER ILKMREGDEL CGQEEAFRTW AKKVFKAACD VFCVGDDVNI
960 970 980 990 1000
EKANNSLISN DRSWKRNKFR LTYVAEAPEL TQGLSNVHKK RVSAARLLSR
1010 1020 1030 1040 1050
QNLQSPKSSR STIFVRLHTN GNQELQYQPG DHLGVFPGNH EDLVNALIER
1060 1070 1080 1090 1100
LEDAPPANHV VKVEMLEERN TALGVISNWK DESRLPPCTI FQAFKYYLDI
1110 1120 1130 1140 1150
TTPPTPLQLQ QFASLATNEK EKQRLLVLSK GLQEYEEWKW GKNPTMVEVL
1160 1170 1180 1190 1200
EEFPSIQMPA TLLLTQLSLL QPRYYSISSS PDMYPDEVHL TVAIVSYHTR
1210 1220 1230 1240 1250
DGEGPVHHGV CSSWLNRIQA DDVVPCFVRG APSFHLPRNP QVPCILVGPG
1260 1270 1280 1290 1300
TGIAPFRSFW QQRQFDIQHK GMNPCPMVLV FGCRQSKIDH IYREETLQAK
1310 1320 1330 1340 1350
NKGVFRELYT AYSREPDRPK KYVQDVLQEQ LAESVYRALK EQGGHIYVCG
1360 1370 1380 1390 1400
DVTMAADVLK AIQRIMTQQG KLSEEDAGVF ISRLRDDNRY HEDIFGVTLR
1410 1420
TYEVTNRLRS ESIAFIEESK KDTDE
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ9Z0J4 | NOS1_MOUSE | Nitric oxide synthase, brain | Nos1 | 1,429 | Annotation score: | ||
F8WGF2 | F8WGF2_MOUSE | Constitutive NOS | Nos1 | 1,463 | Annotation score: | ||
S4R1D5 | S4R1D5_MOUSE | Nitric oxide synthase, brain | Nos1 | 80 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC114617 Genomic DNA No translation available. AC114993 Genomic DNA No translation available. |
Genome annotation databases
Ensembli | ENSMUST00000086451; ENSMUSP00000138506; ENSMUSG00000029361 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC114617 Genomic DNA No translation available. AC114993 Genomic DNA No translation available. |
3D structure databases
SMRi | S4R255 |
ModBasei | Search... |
PTM databases
SwissPalmi | S4R255 |
Proteomic databases
PRIDEi | S4R255 |
Protocols and materials databases
Antibodypediai | 3691, 908 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000086451; ENSMUSP00000138506; ENSMUSG00000029361 |
Organism-specific databases
MGIi | MGI:97360, Nos1 |
Phylogenomic databases
GeneTreei | ENSGT00940000159357 |
HOGENOMi | CLU_001570_16_0_1 |
OMAi | ERGHFYV |
Miscellaneous databases
ChiTaRSi | Nos1, mouse |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029361, Expressed in anterior amygdaloid area and 200 other tissues |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S4R255_MOUSE | |
Accessioni | S4R255Primary (citable) accession number: S4R255 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | September 18, 2013 |
Last sequence update: | September 18, 2013 | |
Last modified: | October 7, 2020 | |
This is version 60 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) |