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Entry version 40 (11 Dec 2019)
Sequence version 1 (18 Sep 2013)
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Protein

Fatty acid synthase apf5

Gene

apf5

Organism
Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Fatty acid synthase; part of the gene cluster that mediates the biosynthesis of the cyclic tetrapeptide apicidin F (APF) (PubMed:25058475). The non-ribosomal peptide synthetase apf1 incorporates four different amino acids to produce apicidin F: L-phenylalanine, D-pipecolic acid (D-pip), N-methoxy-L-tryptophan and L-2-aminooctanedioic acid (PubMed:25058475). L-Phenylalanine is the only proteinogenic amino acid directly used by apf1 (PubMed:24195442, PubMed:25058475). The 3 other apf1 substrates are non-proteinogenic and have to be modified by other enzymes of the cluster (PubMed:25058475). Lysine is converted to delta-1-pyrroline-5-carboxylate (P5C) which is reduced to L-pipecolic acid (L-pip) by apf3 (PubMed:25058475). L-pip is epimerized to D-pip, probably by apf1 activity, prior to incorporation (PubMed:25058475). L-Tryptophan is N-oxidyzed by one of the cytochrome P450 monooxygenases (apf7 or apf8), and further methylated at the hydroxy group by the O-methyltransferase apf6 to yield N-methoxy-L-tryptophan (PubMed:25058475). The synthesis of the fourth apf1 substrate is more complex (PubMed:25058475). The fatty acid synthase apf5 is involved in the synthesis of the octanoic acid backbone of L-2-aminooctanedioic acid by fixing one acetyl-CoA unit and three malonyl-CoA units (PubMed:25058475). Then one of the cytochrome P450 monooxygenases (apf7 or apf8) may oxidize this backbone to 2-oxooctanoic acid (PubMed:25058475). The aminotransferase apf4 is predicted to catalyze the exchange of the keto group with an amino group (PubMed:25058475). The next step would be the oxidation of 2-aminooctanoic acid by one of the cytochrome P450 monooxygenases (apf7 or apf8). The last step is the oxidation of 2-amino-8-hydroxyoctanoic acid to 2-aminooctanedioic acid is catalyzed by the FAD-dependent monooxygenase apf9 (PubMed:25058475).2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Secondary metabolite biosynthesis

This protein is involved in Secondary metabolite biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei1182PROSITE-ProRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Fatty acid synthase apf51 Publication (EC:2.3.1.41PROSITE-ProRule annotation1 Publication)
Alternative name(s):
Apicidin F synthesis protein 51 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:apf51 Publication
ORF Names:FFUJ_00010
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiGibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae and foot rot disease fungus) (Fusarium fujikuroi)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri1279085 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesHypocreomycetidaeHypocrealesNectriaceaeFusariumFusarium fujikuroi species complex
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000016800 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

Apicidin F, like the other known apicidins, is a cyclic tetrapeptides with anti-malarial properties via histone deacetylase inhibitory activity (PubMed:24195442).1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004371601 – 1606Fatty acid synthase apf5Add BLAST1606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei177O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Expression is positively regulated by the apicidin F cluster-specific transcription factor apf2 that binds to the eight-base-pair motif 5'-TGACGTGA-3' called the 'Api-box' that is found in all promoters of the apicidin F cluster except in the promoter region of apf2 itself (PubMed:25058475).1 Publication

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
S0DL59

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini142 – 218CarrierPROSITE-ProRule annotationAdd BLAST77

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.47.10, 3 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR040899 Fas_alpha_ACP
IPR026025 FAS_alpha_yeast
IPR041550 FASI_helical
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR036291 NAD(P)-bd_dom_sf
IPR009081 PP-bd_ACP
IPR016039 Thiolase-like

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF18325 Fas_alpha_ACP, 1 hit
PF18314 FAS_I_H, 1 hit
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000454 FAS_yeast_alpha, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF51735 SSF51735, 1 hit
SSF52151 SSF52151, 1 hit
SSF53901 SSF53901, 2 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit
PS50075 CARRIER, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

S0DL59-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHPDVEQELA YTLLIELMAH QFAYPVRWIE TQDHILGEVN AERIIEIGPS
60 70 80 90 100
PILTNMMKRT IASRFSNSDQ ALNINRHLMS PDNGNPDIYY KYEPTEEPDL
110 120 130 140 150
PELGSQSNTV KVSGSPAWEV QRPVTASHIP SGPVDEIEDT KVPVSAILIS
160 170 180 190 200
LLAPKLKKDP RAIPMTGTIS NLVGGRSTLS NEIVGDLLAE FPNRVPDKPE
210 220 230 240 250
EMPLSSLSET LSTSHDGQLG KATSALVMKM VSSRLPGGYS LSNARLYLRE
260 270 280 290 300
KWGLPPRRQD AVFLLALQRQ PTSRLLSSTD VDSFLDANAA AYFGQENLSI
310 320 330 340 350
PSRGSVQSAA PAVDAKALLL AKQQNDALLR DIMGVIQGHT AAKNESQDAT
360 370 380 390 400
LQASDSEAAA TKKLDMWISE HGDDYAKGMA PIFDAKKQRI YDSYWNWNAQ
410 420 430 440 450
DTILLFQRKL QGQMSSVKEL EQLSTSIVNR ACGRTLEQLD YIIAQAERDP
460 470 480 490 500
TVDSGLLKPM QLLRQTCVET QERQPVFINL SPDMAPLTTI SSDGRLIFSE
510 520 530 540 550
IPRALPCSKI LSTNEASSIA FPVSRADGVA VSYSPQLTEI LCHDLKDSRR
560 570 580 590 600
SGFSFSGKNV LLTGAGEGSI GNHILRHLLA GGARVTVTTS SFSEKVTAMF
610 620 630 640 650
QSIYARHGSK GSVLRVVPFN QGSHGDVQNL VKYIYADASW DLDFILPFAA
660 670 680 690 700
ISENGRDIED LDSKSEIAHR AMLTNLVRLV GAVASQKRQR DTITRPATVV
710 720 730 740 750
LPLSPNHGLL GNDGLYSESK RSLETLIPKW SSETWGSYIA LAGVIIGWTR
760 770 780 790 800
GTGLMDSNDV IAQAVESLGV RTFSAVEMAA NIVSVMGGRF NAECQETPII
810 820 830 840 850
VDMGGGLGSV KNFKAKLTSA RQELNAYAEL KRLTAQESSR DKAYVATDAH
860 870 880 890 900
AKTSKRLRGR ANILLPLPKN LNYEVDIAPF AASLDGMVDL SRVVVITGFA
910 920 930 940 950
ELGPLGNSRT RWEMEESGTL SLEGCVEMAW LMGLITYHNG IDKKGDHYSG
960 970 980 990 1000
WVDTKTSDAI CDDEIPGKYL EFMAKHSGIR EVEPEISDNG YDPSKKESLI
1010 1020 1030 1040 1050
EVALQRDLAP FETSIETAET LKRQHGDKAI VTQDASSGNC QVQLKAGAVV
1060 1070 1080 1090 1100
MVPRASRFNR TVAGQIPLGW TAKRYGISDD IIEQVDPVTL FSLVCTVEAL
1110 1120 1130 1140 1150
LCSGIIDPYE FYQHIHVSQF ANCLGSSMGG LTSLRKMHRD RYLDRSVKSD
1160 1170 1180 1190 1200
LVQETFINTT GAWINMLLSS SSGPIRTPVG ACASSLESLD TACDLIMLKK
1210 1220 1230 1240 1250
AKVCLVGGFE DFVEDLSYEF GCMKATCDTD AEYAAGRVPK EMSRPTASSR
1260 1270 1280 1290 1300
SGFVESQGCG VQVLTSAELA LEMGLPIFGI VAYTSMAADK AGRSVPAPGK
1310 1320 1330 1340 1350
GVLTNAREKS TIPNPMLSLG YRKRLLELRR TQIYQNISDH LDILNSEIAL
1360 1370 1380 1390 1400
FQESETASPG DLKAFRENRE LRIRQDAQAQ DAEVTFSLGN QFWKSQDAGD
1410 1420 1430 1440 1450
ISPIRGSLAV WGLGIDDISV ASLHGTSTVQ NDLNETMVIQ EQMKHLGRRQ
1460 1470 1480 1490 1500
GNLLPCVCQK WLTGHSKGAA GAWMVNGCLQ MMNTGLVPGN RNADNVDEKL
1510 1520 1530 1540 1550
REHRHLAFPN TNLQMDDIKA CSVTSFGFGQ KGGQALLVHP RYLFATIGRE
1560 1570 1580 1590 1600
RYDGYISKRD KRWRKACFRL SEAMVQGNMV SKCIKTEAPY TSADGVAALL

DPTARF
Length:1,606
Mass (Da):175,724
Last modified:September 18, 2013 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9B979110B4A66562
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
HF679023 Genomic DNA Translation: CCT63354.1

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
CCT63354; CCT63354; FFUJ_00010

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HF679023 Genomic DNA Translation: CCT63354.1

3D structure databases

SMRiS0DL59
ModBaseiSearch...

Genome annotation databases

EnsemblFungiiCCT63354; CCT63354; FFUJ_00010

Family and domain databases

Gene3Di3.40.47.10, 3 hits
InterProiView protein in InterPro
IPR016035 Acyl_Trfase/lysoPLipase
IPR040899 Fas_alpha_ACP
IPR026025 FAS_alpha_yeast
IPR041550 FASI_helical
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR036291 NAD(P)-bd_dom_sf
IPR009081 PP-bd_ACP
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF18325 Fas_alpha_ACP, 1 hit
PF18314 FAS_I_H, 1 hit
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit
PIRSFiPIRSF000454 FAS_yeast_alpha, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52151 SSF52151, 1 hit
SSF53901 SSF53901, 2 hits
PROSITEiView protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit
PS50075 CARRIER, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAPF5_GIBF5
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: S0DL59
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: September 18, 2013
Last modified: December 11, 2019
This is version 40 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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