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Protein

Enolase

Gene

eno

Organism
Enterococcus malodoratus ATCC 43197
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno), Enolase (eno)
  5. Pyruvate kinase (UAI_01216)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155SubstrateUniRule annotation1
Binding sitei164SubstrateUniRule annotation1
Active sitei205Proton donorUniRule annotation1
Metal bindingi242MagnesiumUniRule annotation1
Metal bindingi288MagnesiumUniRule annotation1
Binding sitei288SubstrateUniRule annotation1
Metal bindingi315MagnesiumUniRule annotation1
Binding sitei315SubstrateUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340Substrate (covalent); in inhibited formUniRule annotation1
Binding sitei391SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotation
Biological processGlycolysisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
Alternative name(s):
2-phospho-D-glycerate hydro-lyaseUniRule annotation
2-phosphoglycerate dehydrataseUniRule annotation
Gene namesi
Name:enoUniRule annotation
ORF Names:UAI_00664Imported
OrganismiEnterococcus malodoratus ATCC 43197Imported
Taxonomic identifieri1158601 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesEnterococcaceaeEnterococcus
Proteomesi
  • UP000013783 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Secreted UniRule annotation
  • Cell surface UniRule annotation
  • Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation, SecretedUniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 134Enolase_NInterPro annotationAdd BLAST131
Domaini139 – 428Enolase_CInterPro annotationAdd BLAST290

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni367 – 370Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the enolase family.UniRule annotation

Phylogenomic databases

OrthoDBiPOG091H02DK

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

R2RJ57-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIISDVYAR EVLDSRGNPT IEVEVYTEDG AFGRGMVPSG ASTGEYEAVE
60 70 80 90 100
LRDGDKARYG GKGVTKAVDN VNNIIAEAII GYDVRDQAAI DAAMIELDGT
110 120 130 140 150
PNKGKLGANA ILGVSIAVAR AAADFLEIPL YNYLGGFNTK VLPTPMMNII
160 170 180 190 200
NGGSHADNSI DFQEFMIMPV GAPTFKEALR YGAEVFHALA SILKAKGLAT
210 220 230 240 250
SVGDEGGFAP NLGSNEEGFE VIIEAIEKAG YVPGKDIVLA MDAASSEFYD
260 270 280 290 300
KEKGVYVLAD SGEGEKTTEE MIAFYESLVA KYPIISIEDG LDENDWEGTK
310 320 330 340 350
KLTEALGDKV QLVGDDLFVT NTEKLAKAID MGVANSILIK VNQIGTLTET
360 370 380 390 400
FNAIEMAKEA GYTAVVSHRS GETEDSTISD IAVATNAGQI KTGSLSRTDR
410 420 430
IAKYNQLLRI EDQLGDVAEY KGIKSFYNLK NNPFIAK
Length:437
Mass (Da):46,785
Last modified:June 26, 2013 - v1
Checksum:i3ECB1BA5E506D2E8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJAK01000007 Genomic DNA Translation: EOH80626.1
RefSeqiWP_010739546.1, NZ_KE136481.1

Genome annotation databases

EnsemblBacteriaiEOH80626; EOH80626; UAI_00664
GeneIDi34353882
PATRICifig|1158601.3.peg.645

Similar proteinsi

Entry informationi

Entry nameiR2RJ57_9ENTE
AccessioniPrimary (citable) accession number: R2RJ57
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: June 20, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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