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Protein

UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase

Gene

murE

Organism
Ralstonia pickettii OR214
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei194UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei200UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei202UDP-MurNAc-L-Ala-D-GluUniRule annotation1
Binding sitei406Meso-diaminopimelateUniRule annotation1
Binding sitei481Meso-diaminopimelate; via carbonyl oxygenUniRule annotation1
Binding sitei485Meso-diaminopimelateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi125 – 131ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigaseUniRule annotationImported
Biological processCell cycle, Cell divisionUniRule annotation, Cell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligaseUniRule annotation (EC:6.3.2.13UniRule annotation)
Alternative name(s):
Meso-A2pm-adding enzymeUniRule annotation
Meso-diaminopimelate-adding enzymeUniRule annotation
UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligaseUniRule annotation
UDP-MurNAc-tripeptide synthetaseUniRule annotation
UDP-N-acetylmuramyl-tripeptide synthetaseUniRule annotation
Gene namesi
Name:murEUniRule annotation
ORF Names:OR214_03669Imported
OrganismiRalstonia pickettii OR214Imported
Taxonomic identifieri1264675 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000013280 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei234N6-carboxylysineUniRule annotation1

Post-translational modificationi

Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini123 – 331Mur_ligase_MInterPro annotationAdd BLAST209
Domaini352 – 439Mur_ligase_CInterPro annotationAdd BLAST88

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni167 – 168UDP-MurNAc-L-Ala-D-Glu bindingUniRule annotation2
Regioni430 – 433Meso-diaminopimelate bindingUniRule annotation4

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi430 – 433Meso-diaminopimelate recognition motifUniRule annotation4

Sequence similaritiesi

Belongs to the MurCDEF family. MurE subfamily.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiPOG091H0082

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

R0E4U6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAKPTSDRP TIDARLTPVV DWLRAQAPAR ADLTSDTRKL KTGDVFVAYV
60 70 80 90 100
LGNARQRGDG RPHIPQAIEA GASAVLAEAH GYAMPADAPV RILPVDGLAE
110 120 130 140 150
LAGPLAAQWY GVPGPEALRV IGVTGTNGKT SCSQWIAQAL TKHGERCAVV
160 170 180 190 200
GTLGTGFVDA LATTGFTTPD AIQLQRSLAD LHRAGARAIA MEVSSHGLEQ
210 220 230 240 250
GRVDGTRFDI AVFTNLTQDH LDYHGTMAEY ELAKARLFGW QGLRAAVINR
260 270 280 290 300
DDAAGVRLLQ NARADVETIE YGIDGDAAAQ HGARQWLRAT NVRAHRTGTT
310 320 330 340 350
FDVDGSFGRA TVHVSTVGLF NVSNLLAVLG ALLAAGVSWQ DAIARLEKLQ
360 370 380 390 400
PVSGRMERFG GEDGPLVVVD YAHTPDALEQ TLRALAPITE ARGGKLWAVF
410 420 430 440 450
GCGGDRDPGK RPQMGAIAER LAPHVVLTSD NPRSEDPQHI LDEIADGMDN
460 470 480 490 500
PRAATQIEDR AAAILHAVRH ADAHDVIVVA GKGHESTQEI AGRKRPFSDQ
510
EHVRLALAAR GVNA
Length:514
Mass (Da):54,691
Last modified:June 26, 2013 - v1
Checksum:i808828593376034D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
APMQ01000010 Genomic DNA Translation: ENZ76502.1
RefSeqiWP_004633078.1, NZ_APMQ01000010.1

Genome annotation databases

EnsemblBacteriaiENZ76502; ENZ76502; OR214_03669
PATRICifig|1264675.3.peg.3605

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
APMQ01000010 Genomic DNA Translation: ENZ76502.1
RefSeqiWP_004633078.1, NZ_APMQ01000010.1

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiENZ76502; ENZ76502; OR214_03669
PATRICifig|1264675.3.peg.3605

Phylogenomic databases

OrthoDBiPOG091H0082

Enzyme and pathway databases

UniPathwayi
UPA00219

Family and domain databases

Gene3Di3.40.1190.10, 1 hit
3.90.190.20, 1 hit
HAMAPiMF_00208 MurE, 1 hit
InterProiView protein in InterPro
IPR036565 Mur-like_cat_sf
IPR004101 Mur_ligase_C
IPR036615 Mur_ligase_C_dom_sf
IPR013221 Mur_ligase_cen
IPR035911 MurE/MurF_N
IPR005761 UDP-N-AcMur-Glu-dNH2Pim_ligase
PfamiView protein in Pfam
PF02875 Mur_ligase_C, 1 hit
PF08245 Mur_ligase_M, 1 hit
SUPFAMiSSF53244 SSF53244, 1 hit
SSF53623 SSF53623, 1 hit
SSF63418 SSF63418, 1 hit
TIGRFAMsiTIGR01085 murE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiR0E4U6_RALPI
AccessioniPrimary (citable) accession number: R0E4U6
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: March 28, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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