UniProtKB - Q9ZWG1 (PUMP2_ARATH)
Mitochondrial uncoupling protein 2
PUMP2
Functioni
PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. May be involved in protecting plant cells against oxidative stress damage (By similarity).
By similarityGO - Molecular functioni
- amino acid transmembrane transporter activity Source: TAIR
- oxidative phosphorylation uncoupler activity Source: GO_Central
GO - Biological processi
- amino acid transmembrane transport Source: TAIR
- mitochondrial transmembrane transport Source: GO_Central
- mitochondrial transport Source: InterPro
Keywordsi
Biological process | Stress response, Transport |
Protein family/group databases
TCDBi | 2.A.29.3.8, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial uncoupling protein 2Short name: AtPUMP2 |
Gene namesi | Name:PUMP2 Synonyms:UCP2 Ordered Locus Names:At5g58970 ORF Names:K19M22.16 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G58970 |
TAIRi | locus:2154593, AT5G58970 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Golgi apparatus
- Golgi apparatus Source: TAIR
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 16 – 36 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 73 – 93 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 120 – 140 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 179 – 199 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 220 – 240 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 270 – 290 | Helical; Name=6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420256 | 1 – 305 | Mitochondrial uncoupling protein 2Add BLAST | 305 |
Proteomic databases
PaxDbi | Q9ZWG1 |
ProteomicsDBi | 226124 [Q9ZWG1-1] |
Expressioni
Inductioni
Gene expression databases
ExpressionAtlasi | Q9ZWG1, baseline and differential |
Genevisiblei | Q9ZWG1, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 10 – 104 | Solcar 1Add BLAST | 95 | |
Repeati | 114 – 205 | Solcar 2Add BLAST | 92 | |
Repeati | 214 – 297 | Solcar 3Add BLAST | 84 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0753, Eukaryota |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | Q9ZWG1 |
OMAi | MMVTKKR |
OrthoDBi | 984118at2759 |
PhylomeDBi | Q9ZWG1 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADFKPRIEI SFLETFICSA FAACFAELCT IPLDTAKVRL QLQRKIPTGD
60 70 80 90 100
GENLPKYRGS IGTLATIARE EGISGLWKGV IAGLHRQCIY GGLRIGLYEP
110 120 130 140 150
VKTLLVGSDF IGDIPLYQKI LAALLTGAIA IIVANPTDLV KVRLQSEGKL
160 170 180 190 200
PAGVPRRYAG AVDAYFTIVK LEGVSALWTG LGPNIARNAI VNAAELASYD
210 220 230 240 250
QIKETIMKIP FFRDSVLTHL LAGLAAGFFA VCIGSPIDVV KSRMMGDSTY
260 270 280 290 300
RNTVDCFIKT MKTEGIMAFY KGFLPNFTRL GTWNAIMFLT LEQVKKVFLR
EVLYD
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ9FGU9 | Q9FGU9_ARATH | Gb|AAC95187.1 | At5g59020, K19M22.16, K19M22_16 | 780 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044433 | 241 – 272 | KSRMM…AFYKG → SIHFRLLHKSTTRLFGFRLI MCGSAYIYNKFQ in isoform 2. 1 PublicationAdd BLAST | 32 | |
Alternative sequenceiVSP_044434 | 273 – 305 | Missing in isoform 2. 1 PublicationAdd BLAST | 33 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021706 mRNA Translation: BAA36222.1 JQ937241 mRNA Translation: AFI41209.1 AB016885 Genomic DNA Translation: BAB09640.1 CP002688 Genomic DNA Translation: AED97124.1 CP002688 Genomic DNA Translation: AED97125.1 AY088200 mRNA Translation: AAM65742.1 BX831274 mRNA No translation available. |
RefSeqi | NP_568894.1, NM_125287.5 [Q9ZWG1-1] NP_974962.1, NM_203233.1 [Q9ZWG1-2] |
Genome annotation databases
EnsemblPlantsi | AT5G58970.1; AT5G58970.1; AT5G58970 [Q9ZWG1-1] AT5G58970.2; AT5G58970.2; AT5G58970 [Q9ZWG1-2] |
GeneIDi | 836014 |
Gramenei | AT5G58970.1; AT5G58970.1; AT5G58970 [Q9ZWG1-1] AT5G58970.2; AT5G58970.2; AT5G58970 [Q9ZWG1-2] |
KEGGi | ath:AT5G58970 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB021706 mRNA Translation: BAA36222.1 JQ937241 mRNA Translation: AFI41209.1 AB016885 Genomic DNA Translation: BAB09640.1 CP002688 Genomic DNA Translation: AED97124.1 CP002688 Genomic DNA Translation: AED97125.1 AY088200 mRNA Translation: AAM65742.1 BX831274 mRNA No translation available. |
RefSeqi | NP_568894.1, NM_125287.5 [Q9ZWG1-1] NP_974962.1, NM_203233.1 [Q9ZWG1-2] |
3D structure databases
AlphaFoldDBi | Q9ZWG1 |
SMRi | Q9ZWG1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G58970.1 |
Protein family/group databases
TCDBi | 2.A.29.3.8, the mitochondrial carrier (mc) family |
Proteomic databases
PaxDbi | Q9ZWG1 |
ProteomicsDBi | 226124 [Q9ZWG1-1] |
Genome annotation databases
EnsemblPlantsi | AT5G58970.1; AT5G58970.1; AT5G58970 [Q9ZWG1-1] AT5G58970.2; AT5G58970.2; AT5G58970 [Q9ZWG1-2] |
GeneIDi | 836014 |
Gramenei | AT5G58970.1; AT5G58970.1; AT5G58970 [Q9ZWG1-1] AT5G58970.2; AT5G58970.2; AT5G58970 [Q9ZWG1-2] |
KEGGi | ath:AT5G58970 |
Organism-specific databases
Araporti | AT5G58970 |
TAIRi | locus:2154593, AT5G58970 |
Phylogenomic databases
eggNOGi | KOG0753, Eukaryota |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | Q9ZWG1 |
OMAi | MMVTKKR |
OrthoDBi | 984118at2759 |
PhylomeDBi | Q9ZWG1 |
Miscellaneous databases
PROi | PR:Q9ZWG1 |
Gene expression databases
ExpressionAtlasi | Q9ZWG1, baseline and differential |
Genevisiblei | Q9ZWG1, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PUMP2_ARATH | |
Accessioni | Q9ZWG1Primary (citable) accession number: Q9ZWG1 Secondary accession number(s): F4KGF2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2012 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families