Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

12S seed storage protein CRD

Gene

CRD

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seed storage protein.

GO - Molecular functioni

  • nutrient reservoir activity Source: UniProtKB

GO - Biological processi

  • seed maturation Source: UniProtKB

Keywordsi

Molecular functionSeed storage protein, Storage protein

Names & Taxonomyi

Protein namesi
Recommended name:
12S seed storage protein CRD
Alternative name(s):
Cruciferin D
Legumin-type globulin storage protein CRD
Cleaved into the following 2 chains:
Alternative name(s):
12S seed storage protein CRD acidic chain
Alternative name(s):
12S seed storage protein CRD basic chain
Gene namesi
Name:CRD
Synonyms:CRU2
Ordered Locus Names:At1g03890
ORF Names:F21M11.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G03890
TAIRilocus:2024219 AT1G03890

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 252 PublicationsAdd BLAST25
ChainiPRO_000039992527 – 27012S seed storage protein CRD alpha chainAdd BLAST244
ChainiPRO_0000399926271 – 45112S seed storage protein CRD beta chainAdd BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 69By similarity
Modified residuei39Phosphoserine1 Publication1
Disulfide bondi112 ↔ 277Interchain (between alpha and beta chains)Sequence analysis
Modified residuei115PhosphothreonineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei396PhosphothreonineBy similarity1
Modified residuei437PhosphoserineBy similarity1

Post-translational modificationi

Ubiquitinated.By similarity
Proteolytically processed during seed maturation at a conserved Asn-Gly peptide bond by an asparaginyl endopeptidase to produce two mature polypeptides referred to as alpha and beta subunits that are joined together by a disulfide bond.
Phosphorylated in seeds on some Tyr residues in response to abscisic acid (ABA).By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9ZWA9

PTM databases

iPTMnetiQ9ZWA9

Expressioni

Tissue specificityi

Accumulates in seeds 8 days after anthesis.2 Publications

Developmental stagei

First observed in siliques 15 days post anthesis and accumulates progressively as native isoforms or proteolytic fragments during the last week of seed maturation/desiccation. Present in dry seeds, but disappears during their germination (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9ZWA9 differential
GenevisibleiQ9ZWA9 AT

Interactioni

Subunit structurei

Hexamer; each subunit is composed of an acidic and a basic chain derived from a single precursor and linked by a disulfide bond.

Protein-protein interaction databases

STRINGi3702.AT1G03890.1

Structurei

3D structure databases

ProteinModelPortaliQ9ZWA9
SMRiQ9ZWA9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHSE Eukaryota
ENOG410YATH LUCA
HOGENOMiHOG000217279
InParanoidiQ9ZWA9
OMAiGFEWISF
OrthoDBiEOG09360C8E
PhylomeDBiQ9ZWA9

Family and domain databases

Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR006044 11S_seedstore_pln
IPR006045 Cupin_1
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF00190 Cupin_1, 2 hits
PRINTSiPR00439 11SGLOBULIN
SMARTiView protein in SMART
SM00835 Cupin_1, 2 hits
SUPFAMiSSF51182 SSF51182, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ZWA9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKLLFSLLS VVSLSFLLFF HGAEARQREA PFPNACHFSQ INSLAPAQAT
60 70 80 90 100
KFEAGQMEVW DHMSPELRCA GVTVARITLQ PNSIFLPAFF SPPALAYVVQ
110 120 130 140 150
GEGVMGTIAS GCPETFAEVE GSSGRGGGGD PGRRFEDMHQ KLENFRRGDV
160 170 180 190 200
FASLAGVSQW WYNRGDSDAV IVIVLDVTNR ENQLDQVPRM FQLAGSRTQE
210 220 230 240 250
EEQPLTWPSG NNAFSGFDPN IIAEAFKINI ETAKQLQNQK DNRGNIIRAN
260 270 280 290 300
GPLHFVIPPP REWQQDGIAN GIEETYCTAK IHENIDDPER SDHFSTRAGR
310 320 330 340 350
ISTLNSLNLP VLRLVRLNAL RGYLYSGGMV LPQWTANAHT VLYVTGGQAK
360 370 380 390 400
IQVVDDNGQS VFNEQVGQGQ IIVIPQGFAV SKTAGETGFE WISFKTNDNA
410 420 430 440 450
YINTLSGQTS YLRAVPVDVI KASYGVNEEE AKRIKFSQQE TMLSMTPSSS

S
Length:451
Mass (Da):49,675
Last modified:May 1, 1999 - v1
Checksum:i456BA2B5BE8BB9B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti382K → R in BAD43747 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003027 Genomic DNA Translation: AAD10679.1
CP002684 Genomic DNA Translation: AEE27629.1
AY065432 mRNA Translation: AAL38873.1
AY117228 mRNA Translation: AAM51303.1
AK175984 mRNA Translation: BAD43747.1
PIRiF86169
RefSeqiNP_171885.1, NM_100269.4
UniGeneiAt.20175

Genome annotation databases

EnsemblPlantsiAT1G03890.1; AT1G03890.1; AT1G03890
GeneIDi839379
GrameneiAT1G03890.1; AT1G03890.1; AT1G03890
KEGGiath:AT1G03890

Similar proteinsi

Entry informationi

Entry nameiCRU4_ARATH
AccessioniPrimary (citable) accession number: Q9ZWA9
Secondary accession number(s): Q67ZY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health