UniProtKB - Q9ZUY3 (AROD3_ARATH)
Protein
Arogenate dehydratase 3, chloroplastic
Gene
ADT3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine. Together with GCR1 and GPA1, required for blue light-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings.2 Publications
Miscellaneous
Has no detectable prehenate dehydratase activity.
Caution
Was reported to be a cytosolic prephenate dehydratase interacting with a G-protein alpha-subunit.1 Publication
Catalytic activityi
- EC:4.2.1.91
Kineticsi
- KM=0.43 mM for arogenate1 Publication
- Vmax=5.17 pmol/sec/µg enzyme with arogenate as substrate1 Publication
: L-phenylalanine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes L-phenylalanine from L-arogenate.Proteins known to be involved in this subpathway in this organism are:
- Arogenate dehydratase (AXX17_At2g23810), Arogenate dehydratase 5, chloroplastic (ADT5), Arogenate dehydratase 4, chloroplastic (ADT4), Arogenate dehydratase (AT9943_LOCUS7865), Arogenate dehydratase (AT9943_LOCUS19800), Arogenate dehydratase (AXX17_At3g07630), Arogenate dehydratase/prephenate dehydratase 6, chloroplastic (ADT6), Arogenate dehydratase (AXX17_At1g12160), Arogenate dehydratase (AXX17_At3g38410), Arogenate dehydratase (AXX17_At1g08050), Arogenate dehydratase/prephenate dehydratase 1, chloroplastic (ADT1), Arogenate dehydratase (AXX17_At5g22060), Arogenate dehydratase 3, chloroplastic (ADT3), Arogenate dehydratase/prephenate dehydratase 2, chloroplastic (ADT2), Arogenate dehydratase (AT9943_LOCUS10400), Arogenate dehydratase (AT9943_LOCUS12741)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-phenylalanine from L-arogenate, the pathway L-phenylalanine biosynthesis and in Amino-acid biosynthesis.
GO - Molecular functioni
- arogenate dehydratase activity Source: TAIR
- prephenate dehydratase activity Source: InterPro
GO - Biological processi
- L-phenylalanine biosynthetic process Source: TAIR
- response to karrikin Source: TAIR
- response to low fluence blue light stimulus by blue low-fluence system Source: TAIR
- tyrosine biosynthetic process Source: TAIR
Keywordsi
Molecular function | Lyase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Phenylalanine biosynthesis |
Enzyme and pathway databases
BioCyci | ARA:AT2G27820-MONOMER |
BRENDAi | 4.2.1.51, 399 4.2.1.91, 399 |
SABIO-RKi | Q9ZUY3 |
UniPathwayi | UPA00121;UER00344 |
Names & Taxonomyi
Protein namesi | Recommended name: Arogenate dehydratase 3, chloroplastic (EC:4.2.1.91)Short name: AtADT3 Alternative name(s): Prephenate dehydratase 1 Short name: AtPDT1 |
Gene namesi | Name:ADT3 Synonyms:PD1, PDT1 Ordered Locus Names:At2g27820 ORF Names:F15K20.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G27820 |
TAIRi | locus:2042021, AT2G27820 |
Subcellular locationi
Chloroplast
- chloroplast stroma 2 Publications
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: UniProtKB-SubCell
Cytosol
- cytosol Source: TAIR
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Lack of Phe and Tyr accumulation after blue light irradiation of etiolated seedlings.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 24 | ChloroplastSequence analysisAdd BLAST | 24 | |
ChainiPRO_0000373792 | 25 – 424 | Arogenate dehydratase 3, chloroplasticAdd BLAST | 400 |
Proteomic databases
PaxDbi | Q9ZUY3 |
PRIDEi | Q9ZUY3 |
ProteomicsDBi | 246999 |
Expressioni
Tissue specificityi
Expressed in roots, leaves, stems, flowers and siliques.1 Publication
Gene expression databases
ExpressionAtlasi | Q9ZUY3, baseline and differential |
Genevisiblei | Q9ZUY3, AT |
Interactioni
Subunit structurei
May interact with GPA1.
Protein-protein interaction databases
BioGRIDi | 2679, 1 interactor |
STRINGi | 3702.AT2G27820.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 122 – 299 | Prephenate dehydratasePROSITE-ProRule annotationAdd BLAST | 178 | |
Domaini | 313 – 404 | ACTPROSITE-ProRule annotationAdd BLAST | 92 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 30 – 75 | Ser-richAdd BLAST | 46 | |
Compositional biasi | 241 – 272 | Ala-richAdd BLAST | 32 | |
Compositional biasi | 409 – 420 | Poly-SerAdd BLAST | 12 |
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2797, Eukaryota |
HOGENOMi | CLU_035008_4_1_1 |
InParanoidi | Q9ZUY3 |
OMAi | CEHTISK |
OrthoDBi | 1090069at2759 |
PhylomeDBi | Q9ZUY3 |
Family and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001086, Preph_deHydtase IPR018528, Preph_deHydtase_CS |
Pfami | View protein in Pfam PF00800, PDT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00857, PREPHENATE_DEHYDR_1, 1 hit PS00858, PREPHENATE_DEHYDR_2, 1 hit PS51171, PREPHENATE_DEHYDR_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9ZUY3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRTLLPSHTP ATVTTAARRR HVIHCAGKRS DSFSINSSSS DWQSSCAILS
60 70 80 90 100
SKVNSQEQSE SLSSNSNGSS SYHVSAVNGH NNGAGVSDLN LVPFNNNQSI
110 120 130 140 150
QSKKPLSISD LSPAPMHGSN LRVAYQGVPG AYSEAAAGKA YPNCQAIPCD
160 170 180 190 200
QFEVAFQAVE LWIADRAVLP VENSLGGSIH RNYDLLLRHR LHIVGEVQLP
210 220 230 240 250
VHHCLIALPG VRKEFLTRVI SHPQGLAQCE HTLTKLGLNV AREAVDDTAG
260 270 280 290 300
AAEFIAANNI RDTAAIASAR AAEIYGLEIL EDGIQDDASN VTRFVMLARE
310 320 330 340 350
PIIPRTDRPF KTSIVFAHEK GTCVLFKVLS AFAFRNISLT KIESRPNHNV
360 370 380 390 400
PIRLVDEANV GTAKHFEYMF YIDFEASMAE SRAQNALSEV QEFTSFLRVL
410 420
GSYPMDMTSW SPSSSSSSSS TFSL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 206 | I → M in AAM65232 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ411464 mRNA Translation: ABD67750.1 AC005824 Genomic DNA Translation: AAC73018.1 CP002685 Genomic DNA Translation: AEC08050.1 AY087695 mRNA Translation: AAM65232.1 BT025989 mRNA Translation: ABG25078.1 AK229426 mRNA Translation: BAF01286.1 |
PIRi | D84677 |
RefSeqi | NP_180350.1, NM_128342.3 |
Genome annotation databases
EnsemblPlantsi | AT2G27820.1; AT2G27820.1; AT2G27820 |
GeneIDi | 817329 |
Gramenei | AT2G27820.1; AT2G27820.1; AT2G27820 |
KEGGi | ath:AT2G27820 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ411464 mRNA Translation: ABD67750.1 AC005824 Genomic DNA Translation: AAC73018.1 CP002685 Genomic DNA Translation: AEC08050.1 AY087695 mRNA Translation: AAM65232.1 BT025989 mRNA Translation: ABG25078.1 AK229426 mRNA Translation: BAF01286.1 |
PIRi | D84677 |
RefSeqi | NP_180350.1, NM_128342.3 |
3D structure databases
SMRi | Q9ZUY3 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2679, 1 interactor |
STRINGi | 3702.AT2G27820.1 |
Proteomic databases
PaxDbi | Q9ZUY3 |
PRIDEi | Q9ZUY3 |
ProteomicsDBi | 246999 |
Genome annotation databases
EnsemblPlantsi | AT2G27820.1; AT2G27820.1; AT2G27820 |
GeneIDi | 817329 |
Gramenei | AT2G27820.1; AT2G27820.1; AT2G27820 |
KEGGi | ath:AT2G27820 |
Organism-specific databases
Araporti | AT2G27820 |
TAIRi | locus:2042021, AT2G27820 |
Phylogenomic databases
eggNOGi | KOG2797, Eukaryota |
HOGENOMi | CLU_035008_4_1_1 |
InParanoidi | Q9ZUY3 |
OMAi | CEHTISK |
OrthoDBi | 1090069at2759 |
PhylomeDBi | Q9ZUY3 |
Enzyme and pathway databases
UniPathwayi | UPA00121;UER00344 |
BioCyci | ARA:AT2G27820-MONOMER |
BRENDAi | 4.2.1.51, 399 4.2.1.91, 399 |
SABIO-RKi | Q9ZUY3 |
Miscellaneous databases
PROi | PR:Q9ZUY3 |
Gene expression databases
ExpressionAtlasi | Q9ZUY3, baseline and differential |
Genevisiblei | Q9ZUY3, AT |
Family and domain databases
InterProi | View protein in InterPro IPR002912, ACT_dom IPR001086, Preph_deHydtase IPR018528, Preph_deHydtase_CS |
Pfami | View protein in Pfam PF00800, PDT, 1 hit |
PROSITEi | View protein in PROSITE PS51671, ACT, 1 hit PS00857, PREPHENATE_DEHYDR_1, 1 hit PS00858, PREPHENATE_DEHYDR_2, 1 hit PS51171, PREPHENATE_DEHYDR_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROD3_ARATH | |
Accessioni | Q9ZUY3Primary (citable) accession number: Q9ZUY3 Secondary accession number(s): Q0WNL3, Q8LAP1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways