UniProtKB - Q9ZU51 (RHA2B_ARATH)
Protein
E3 ubiquitin-protein ligase RHA2B
Gene
RHA2B
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
E3 ubiquitin-protein ligase involved in the positive regulation of abscisic acid (ABA) signaling and responses to salt and osmotic stresses during seed germination and early seedling development. Acts additively with RHA2A in regulating ABA signaling and drought response. Possesses E3 ubiquitin ligase activity in vitro.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 74 – 116 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 43 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: UniProtKB
- ubiquitin-protein transferase activity Source: TAIR
GO - Biological processi
- abscisic acid-activated signaling pathway Source: UniProtKB-KW
- cellular response to hypoxia Source: TAIR
- positive regulation of abscisic acid-activated signaling pathway Source: UniProtKB
- protein ubiquitination Source: UniProtKB
- regulation of response to osmotic stress Source: UniProtKB
- response to karrikin Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Abscisic acid signaling pathway, Stress response, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RHA2BCurated (EC:2.3.2.27)Alternative name(s): RING-H2 finger A2b1 Publication RING-H2 zinc finger protein RHA2bCurated RING-type E3 ubiquitin transferase RHA2BCurated |
Gene namesi | Name:RHA2B1 Publication Ordered Locus Names:At2g01150 ORF Names:F10A8.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G01150 |
TAIRi | locus:2038766, AT2G01150 |
Pathology & Biotechi
Disruption phenotypei
No visible phenotype.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056036 | 1 – 147 | E3 ubiquitin-protein ligase RHA2BAdd BLAST | 147 |
Proteomic databases
PaxDbi | Q9ZU51 |
ProteomicsDBi | 236901 |
PTM databases
iPTMneti | Q9ZU51 |
Expressioni
Tissue specificityi
Expressed in vascular tissue, root tips, embryos and pistils.1 Publication
Inductioni
By the proteasome inhibitor MG132. Down-regulated during reinitiation of mitotic activity.1 Publication
Gene expression databases
ExpressionAtlasi | Q9ZU51, baseline and differential |
Genevisiblei | Q9ZU51, AT |
Interactioni
Subunit structurei
Interacts with NAC19.
1 PublicationProtein-protein interaction databases
BioGRIDi | 48, 1 interactor |
IntActi | Q9ZU51, 1 interactor |
STRINGi | 3702.AT2G01150.1 |
Family & Domainsi
Domaini
The ring domain is sufficient for the interaction with NAC19.
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 74 – 116 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 43 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
HOGENOMi | CLU_013137_18_5_1 |
InParanoidi | Q9ZU51 |
OMAi | CLCTLRD |
PhylomeDBi | Q9ZU51 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9ZU51-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLQGQLSDV SSDSIPLMLL ALLATFFRHV RSLLLFPSSA PVVVVTSNLS
60 70 80 90 100
VLADQLNLNR LFSYRYSDNA ASDCIVCLSK LKTGEEVRKL DCRHVFHKQC
110 120 130 140
LEGWLQHLNF NCPLCRSPLL PHHHQGHGSD ASISAFPLRS TSTASSH
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B063 | A0A1P8B063_ARATH | RING-H2 finger protein 2B | RHA2B RING-H2 finger protein 2B, RING-H2 FINGER PROTEIN RHA2B, At2g01150, F10A8.3, F10A8_3 | 196 | Annotation score: | ||
A0A1P8B060 | A0A1P8B060_ARATH | RING-H2 finger protein 2B | RHA2B RING-H2 finger protein 2B, RING-H2 FINGER PROTEIN RHA2B, At2g01150, F10A8.3, F10A8_3 | 173 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078823 mRNA Translation: AAC68672.1 AC006200 Genomic DNA Translation: AAD14516.2 CP002685 Genomic DNA Translation: AEC05407.1 |
PIRi | C84421 T51843 |
RefSeqi | NP_001318169.1, NM_001335035.1 |
Genome annotation databases
EnsemblPlantsi | AT2G01150.1; AT2G01150.1; AT2G01150 |
GeneIDi | 814644 |
Gramenei | AT2G01150.1; AT2G01150.1; AT2G01150 |
KEGGi | ath:AT2G01150 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078823 mRNA Translation: AAC68672.1 AC006200 Genomic DNA Translation: AAD14516.2 CP002685 Genomic DNA Translation: AEC05407.1 |
PIRi | C84421 T51843 |
RefSeqi | NP_001318169.1, NM_001335035.1 |
3D structure databases
SMRi | Q9ZU51 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 48, 1 interactor |
IntActi | Q9ZU51, 1 interactor |
STRINGi | 3702.AT2G01150.1 |
PTM databases
iPTMneti | Q9ZU51 |
Proteomic databases
PaxDbi | Q9ZU51 |
ProteomicsDBi | 236901 |
Genome annotation databases
EnsemblPlantsi | AT2G01150.1; AT2G01150.1; AT2G01150 |
GeneIDi | 814644 |
Gramenei | AT2G01150.1; AT2G01150.1; AT2G01150 |
KEGGi | ath:AT2G01150 |
Organism-specific databases
Araporti | AT2G01150 |
TAIRi | locus:2038766, AT2G01150 |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
HOGENOMi | CLU_013137_18_5_1 |
InParanoidi | Q9ZU51 |
OMAi | CLCTLRD |
PhylomeDBi | Q9ZU51 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q9ZU51 |
Gene expression databases
ExpressionAtlasi | Q9ZU51, baseline and differential |
Genevisiblei | Q9ZU51, AT |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHA2B_ARATH | |
Accessioni | Q9ZU51Primary (citable) accession number: Q9ZU51 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | June 1, 2002 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways