UniProtKB - Q9ZT91 (EFTM_ARATH)
Protein
Elongation factor Tu, mitochondrial
Gene
TUFA
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 74 – 81 | GTPBy similarity | 8 | |
Nucleotide bindingi | 136 – 140 | GTPBy similarity | 5 | |
Nucleotide bindingi | 191 – 194 | GTPBy similarity | 4 |
GO - Molecular functioni
- ATP binding Source: TAIR
- cobalt ion binding Source: TAIR
- GTPase activity Source: InterPro
- GTP binding Source: UniProtKB-KW
- translation elongation factor activity Source: GO_Central
- zinc ion binding Source: TAIR
GO - Biological processi
- mitochondrial translational elongation Source: GO_Central
- response to cadmium ion Source: TAIR
- translational elongation Source: GO_Central
Keywordsi
Molecular function | Elongation factor |
Biological process | Protein biosynthesis |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Elongation factor Tu, mitochondrial |
Gene namesi | Name:TUFA Ordered Locus Names:At4g02930 ORF Names:T4I9.19, T5J8.25 |
Organismi | Arabidopsis thaliana (Mouse-ear cress)Imported |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G02930 |
TAIRi | locus:2139325, AT4G02930 |
Subcellular locationi
Mitochondrion
- Mitochondrion 2 Publications
Cell Wall
- cell wall Source: TAIR
Cytosol
- cytosol Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 51 | MitochondrionSequence analysisAdd BLAST | 51 | |
ChainiPRO_0000007466 | 52 – 454 | Elongation factor Tu, mitochondrialAdd BLAST | 403 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9ZT91 |
PRIDEi | Q9ZT91 |
ProteomicsDBi | 220738 |
PTM databases
MetOSitei | Q9ZT91 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9ZT91, baseline and differential |
Genevisiblei | Q9ZT91, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 13423, 7 interactors |
STRINGi | 3702.AT4G02930.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 65 – 261 | tr-type GAdd BLAST | 197 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 74 – 81 | G1By similarity | 8 | |
Regioni | 115 – 119 | G2By similarity | 5 | |
Regioni | 136 – 139 | G3By similarity | 4 | |
Regioni | 191 – 194 | G4By similarity | 4 | |
Regioni | 229 – 231 | G5By similarity | 3 |
Sequence similaritiesi
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0460, Eukaryota |
HOGENOMi | CLU_007265_0_0_1 |
OMAi | MECELIG |
OrthoDBi | 491836at2759 |
PhylomeDBi | Q9ZT91 |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
HAMAPi | MF_00118_B, EF_Tu_B, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9ZT91-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASVVLRNPS SKRLVPFSSQ IYSRCGASVT SSYSISHSIG GDDLSSSTFG
60 70 80 90 100
TSSFWRSMAT FTRNKPHVNV GTIGHVDHGK TTLTAAITKV LAEEGKAKAI
110 120 130 140 150
AFDEIDKAPE EKKRGITIAT AHVEYETAKR HYAHVDCPGH ADYVKNMITG
160 170 180 190 200
AAQMDGGILV VSGPDGPMPQ TKEHILLARQ VGVPSLVCFL NKVDVVDDPE
210 220 230 240 250
LLELVEMELR ELLSFYKFPG DDIPIIRGSA LSALQGTNDE IGRQAILKLM
260 270 280 290 300
DAVDEYIPDP VRVLDKPFLM PIEDVFSIQG RGTVATGRIE QGVIKVGEEV
310 320 330 340 350
EILGLREGGV PLKSTVTGVE MFKKILDNGQ AGDNVGLLLR GLKREDIQRG
360 370 380 390 400
MVIAKPGSCK TYKKFEAEIY VLTKDEGGRH TAFFSNYRPQ FYLRTADITG
410 420 430 440 450
KVELPENVKM VMPGDNVTAV FELIMPVPLE TGQRFALREG GRTVGAGVVS
KVMT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 – 10 | MASVVLRNPS → MEEPVDREDIDLRVSSTDIG WNEFAAA in CAA61511 (PubMed:8555448).Curated | 10 | |
Sequence conflicti | 310 – 312 | VPL → CST in CAA61511 (PubMed:8555448).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89227 mRNA Translation: CAA61511.1 AC004044 Genomic DNA Translation: AAD15337.1 AF069442 Genomic DNA Translation: AAC79113.1 AL161495 Genomic DNA Translation: CAB77778.1 CP002687 Genomic DNA Translation: AEE82252.1 AY136421 mRNA Translation: AAM97087.1 BT008755 mRNA Translation: AAP49517.1 F14375 mRNA Translation: CAA23078.1 |
PIRi | T01400 |
RefSeqi | NP_192202.1, NM_116527.4 |
Genome annotation databases
EnsemblPlantsi | AT4G02930.1; AT4G02930.1; AT4G02930 |
GeneIDi | 828134 |
Gramenei | AT4G02930.1; AT4G02930.1; AT4G02930 |
KEGGi | ath:AT4G02930 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X89227 mRNA Translation: CAA61511.1 AC004044 Genomic DNA Translation: AAD15337.1 AF069442 Genomic DNA Translation: AAC79113.1 AL161495 Genomic DNA Translation: CAB77778.1 CP002687 Genomic DNA Translation: AEE82252.1 AY136421 mRNA Translation: AAM97087.1 BT008755 mRNA Translation: AAP49517.1 F14375 mRNA Translation: CAA23078.1 |
PIRi | T01400 |
RefSeqi | NP_192202.1, NM_116527.4 |
3D structure databases
SMRi | Q9ZT91 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 13423, 7 interactors |
STRINGi | 3702.AT4G02930.1 |
PTM databases
MetOSitei | Q9ZT91 |
Proteomic databases
PaxDbi | Q9ZT91 |
PRIDEi | Q9ZT91 |
ProteomicsDBi | 220738 |
Genome annotation databases
EnsemblPlantsi | AT4G02930.1; AT4G02930.1; AT4G02930 |
GeneIDi | 828134 |
Gramenei | AT4G02930.1; AT4G02930.1; AT4G02930 |
KEGGi | ath:AT4G02930 |
Organism-specific databases
Araporti | AT4G02930 |
TAIRi | locus:2139325, AT4G02930 |
Phylogenomic databases
eggNOGi | KOG0460, Eukaryota |
HOGENOMi | CLU_007265_0_0_1 |
OMAi | MECELIG |
OrthoDBi | 491836at2759 |
PhylomeDBi | Q9ZT91 |
Miscellaneous databases
PROi | PR:Q9ZT91 |
Gene expression databases
ExpressionAtlasi | Q9ZT91, baseline and differential |
Genevisiblei | Q9ZT91, AT |
Family and domain databases
CDDi | cd01884, EF_Tu, 1 hit cd03697, EFTU_II, 1 hit |
HAMAPi | MF_00118_B, EF_Tu_B, 1 hit |
InterProi | View protein in InterPro IPR041709, EF-Tu_GTP-bd IPR004161, EFTu-like_2 IPR033720, EFTU_2 IPR031157, G_TR_CS IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR000795, T_Tr_GTP-bd_dom IPR009000, Transl_B-barrel_sf IPR009001, Transl_elong_EF1A/Init_IF2_C IPR004541, Transl_elong_EFTu/EF1A_bac/org IPR004160, Transl_elong_EFTu/EF1A_C |
Pfami | View protein in Pfam PF00009, GTP_EFTU, 1 hit PF03144, GTP_EFTU_D2, 1 hit PF03143, GTP_EFTU_D3, 1 hit |
PRINTSi | PR00315, ELONGATNFCT |
SUPFAMi | SSF50447, SSF50447, 1 hit SSF50465, SSF50465, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00485, EF-Tu, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS00301, G_TR_1, 1 hit PS51722, G_TR_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EFTM_ARATH | |
Accessioni | Q9ZT91Primary (citable) accession number: Q9ZT91 Secondary accession number(s): Q39206 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 158 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families