UniProtKB - Q9ZT50 (RHA2A_ARATH)
Protein
E3 ubiquitin-protein ligase RHA2A
Gene
RHA2A
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
E3 ubiquitin-protein ligase involved in the positive regulation of abscisic acid (ABA) signaling and responses to salt and osmotic stresses during seed germination and early seedling development (PubMed:19286935, PubMed:21478367). Acts additively with RHA2B in regulating ABA signaling and drought response (PubMed:21478367). Possesses E3 ubiquitin ligase activity in vitro (PubMed:15644464, PubMed:19286935).3 Publications
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 86 – 128 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 43 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: GO_Central
- ubiquitin-protein transferase activity Source: UniProtKB
GO - Biological processi
- abscisic acid-activated signaling pathway Source: UniProtKB-KW
- positive regulation of abscisic acid-activated signaling pathway Source: TAIR
- protein ubiquitination Source: UniProtKB
- regulation of response to osmotic stress Source: TAIR
- response to salt stress Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Abscisic acid signaling pathway, Stress response, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RHA2ACurated (EC:2.3.2.27)Alternative name(s): RING-H2 finger A2a1 Publication RING-H2 zinc finger protein RHA2aCurated RING-type E3 ubiquitin transferase RHA2ACurated |
Gene namesi | Name:RHA2A1 Publication Ordered Locus Names:At1g15100 ORF Names:F9L1.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G15100 |
TAIRi | locus:2037683, AT1G15100 |
Pathology & Biotechi
Disruption phenotypei
Decreased sensitivity to ABA and salt and osmotic stresses during seed germination and early seedling development.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 89 | C → A: Loss of E3 ubiquitin ligase activity. 1 Publication | 1 | |
Mutagenesisi | 103 | E → P: Decreased affinity for ANAC. 1 Publication | 1 | |
Mutagenesisi | 110 – 111 | KK → NE: Abolishes the nuclear concentration. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056035 | 1 – 155 | E3 ubiquitin-protein ligase RHA2AAdd BLAST | 155 |
Proteomic databases
PaxDbi | Q9ZT50 |
ProteomicsDBi | 236973 |
Expressioni
Tissue specificityi
Expressed in stems, flowers, cauline leaves, rosettes, siliques, seeds and roots.3 Publications
Gene expression databases
ExpressionAtlasi | Q9ZT50, baseline and differential |
Genevisiblei | Q9ZT50, AT |
Interactioni
Subunit structurei
Interacts with NAC019 and NAC055.
2 PublicationsBinary interactionsi
Q9ZT50
With | #Exp. | IntAct |
---|---|---|
NAC019 [Q9C932] | 3 | EBI-2025293,EBI-1786615 |
Protein-protein interaction databases
BioGRIDi | 23313, 1 interactor |
IntActi | Q9ZT50, 1 interactor |
STRINGi | 3702.AT1G15100.1 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 77 – 83 | Poly-Gly | 7 |
Domaini
The ring domain is sufficient for the interaction with ANAC.
Presence of both a nuclear localization sequence (NLS) and a nuclear export sequence (NES).
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 86 – 128 | RING-type; atypicalPROSITE-ProRule annotationAdd BLAST | 43 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0800, Eukaryota |
HOGENOMi | CLU_013137_18_5_1 |
InParanoidi | Q9ZT50 |
OMAi | KLECRHV |
OrthoDBi | 1522472at2759 |
PhylomeDBi | Q9ZT50 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9ZT50-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGLQGQLSDV SSDSIPLMLL SLLAVFINHL RSFLLRLTSK SNPNLPVDDV
60 70 80 90 100
SIASGLANII VLADQLSLNR LFSYRCGDGG GGGSDCVVCL SKLKEGEEVR
110 120 130 140 150
KLECRHVFHK KCLEGWLHQF NFTCPLCRSA LVSDDCVSKT QRSVGRDLIS
CFSLH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078822 mRNA Translation: AAC68671.1 DQ059097 mRNA Translation: AAY57583.1 AC007591 Genomic DNA Translation: AAD39638.1 CP002684 Genomic DNA Translation: AEE29264.1 AY075590 mRNA Translation: AAL91611.1 AY094057 mRNA Translation: AAM16213.1 AY088303 mRNA Translation: AAM65842.1 |
PIRi | T51842 |
RefSeqi | NP_172962.1, NM_101378.3 |
Genome annotation databases
EnsemblPlantsi | AT1G15100.1; AT1G15100.1; AT1G15100 |
GeneIDi | 838073 |
Gramenei | AT1G15100.1; AT1G15100.1; AT1G15100 |
KEGGi | ath:AT1G15100 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF078822 mRNA Translation: AAC68671.1 DQ059097 mRNA Translation: AAY57583.1 AC007591 Genomic DNA Translation: AAD39638.1 CP002684 Genomic DNA Translation: AEE29264.1 AY075590 mRNA Translation: AAL91611.1 AY094057 mRNA Translation: AAM16213.1 AY088303 mRNA Translation: AAM65842.1 |
PIRi | T51842 |
RefSeqi | NP_172962.1, NM_101378.3 |
3D structure databases
SMRi | Q9ZT50 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 23313, 1 interactor |
IntActi | Q9ZT50, 1 interactor |
STRINGi | 3702.AT1G15100.1 |
Proteomic databases
PaxDbi | Q9ZT50 |
ProteomicsDBi | 236973 |
Genome annotation databases
EnsemblPlantsi | AT1G15100.1; AT1G15100.1; AT1G15100 |
GeneIDi | 838073 |
Gramenei | AT1G15100.1; AT1G15100.1; AT1G15100 |
KEGGi | ath:AT1G15100 |
Organism-specific databases
Araporti | AT1G15100 |
TAIRi | locus:2037683, AT1G15100 |
Phylogenomic databases
eggNOGi | KOG0800, Eukaryota |
HOGENOMi | CLU_013137_18_5_1 |
InParanoidi | Q9ZT50 |
OMAi | KLECRHV |
OrthoDBi | 1522472at2759 |
PhylomeDBi | Q9ZT50 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q9ZT50 |
Gene expression databases
ExpressionAtlasi | Q9ZT50, baseline and differential |
Genevisiblei | Q9ZT50, AT |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF13639, zf-RING_2, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RHA2A_ARATH | |
Accessioni | Q9ZT50Primary (citable) accession number: Q9ZT50 Secondary accession number(s): Q4TU39 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways