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Protein

Beta sliding clamp

Gene

dnaN

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciHPYL85963:G1G1A-487-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:jhp_0452
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054391 – 374Beta sliding clampAdd BLAST374

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Protein-protein interaction databases

STRINGi85963.jhp0452

Structurei

3D structure databases

ProteinModelPortaliQ9ZLX4
SMRiQ9ZLX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8 Bacteria
COG0592 LUCA
KOiK02338
OMAiNYEAVIP
OrthoDBiPOG091H02B3

Family and domain databases

CDDicd00140 beta_clamp, 1 hit
InterProiView protein in InterPro
IPR001001 DNA_polIII_beta
IPR022635 DNA_polIII_beta_C
IPR022637 DNA_polIII_beta_cen
IPR022634 DNA_polIII_beta_N
PANTHERiPTHR30478 PTHR30478, 1 hit
PfamiView protein in Pfam
PF00712 DNA_pol3_beta, 1 hit
PF02767 DNA_pol3_beta_2, 1 hit
PF02768 DNA_pol3_beta_3, 1 hit
SMARTiView protein in SMART
SM00480 POL3Bc, 1 hit
TIGRFAMsiTIGR00663 dnan, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZLX4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKISVSKNDL ENTLRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYISTQ STDKEGVGTI NGKKFLDIIS CLKDSNIVLE TKDDSLVIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSLEIN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGVLMQ FNQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVVENET HAFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYTSSFTLG KEEFKEGIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLK CNEPSSPFLI
360 370
QEPLDEKQSH LNAKISTLMM PITL
Length:374
Mass (Da):42,047
Last modified:May 1, 1999 - v1
Checksum:iB0D55FE670AD55D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06030.1
PIRiA71931
RefSeqiWP_000704288.1, NC_000921.1

Genome annotation databases

EnsemblBacteriaiAAD06030; AAD06030; jhp_0452
KEGGihpj:jhp_0452

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06030.1
PIRiA71931
RefSeqiWP_000704288.1, NC_000921.1

3D structure databases

ProteinModelPortaliQ9ZLX4
SMRiQ9ZLX4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85963.jhp0452

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD06030; AAD06030; jhp_0452
KEGGihpj:jhp_0452

Phylogenomic databases

eggNOGiENOG4105CZ8 Bacteria
COG0592 LUCA
KOiK02338
OMAiNYEAVIP
OrthoDBiPOG091H02B3

Enzyme and pathway databases

BioCyciHPYL85963:G1G1A-487-MONOMER

Family and domain databases

CDDicd00140 beta_clamp, 1 hit
InterProiView protein in InterPro
IPR001001 DNA_polIII_beta
IPR022635 DNA_polIII_beta_C
IPR022637 DNA_polIII_beta_cen
IPR022634 DNA_polIII_beta_N
PANTHERiPTHR30478 PTHR30478, 1 hit
PfamiView protein in Pfam
PF00712 DNA_pol3_beta, 1 hit
PF02767 DNA_pol3_beta_2, 1 hit
PF02768 DNA_pol3_beta_3, 1 hit
SMARTiView protein in SMART
SM00480 POL3Bc, 1 hit
TIGRFAMsiTIGR00663 dnan, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3B_HELPJ
AccessioniPrimary (citable) accession number: Q9ZLX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: February 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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