murI

Functionⁱ

Provides the (R)-glutamate required for cell wall biosynthesis.UniRule annotation1 Publication

Catalytic activityⁱ

L-glutamate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
=
D-glutamate
Search proteins in UniProtKB for this molecule.
Search chemical reactions in Rhea for this molecule.
See the description of this molecule in ChEBI.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00258
3 Publications
Manual assertion based on experiment inⁱ
- Ref.2
  "Exploitation of structural and regulatory diversity in glutamate racemases."
  Lundqvist T., Fisher S.L., Kern G., Folmer R.H., Xue Y., Newton D.T., Keating T.A., Alm R.A., de Jonge B.L.
  Nature 447:817-822(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY, SUBUNIT, FUNCTION, PATHWAY.
- Ref.3
  "Potent and selective inhibitors of Helicobacter pylori glutamate racemase (MurI): pyridodiazepine amines."
  Geng B., Basarab G., Comita-Prevoir J., Gowravaram M., Hill P., Kiely A., Loch J., MacPherson L., Morningstar M., Mullen G., Osimboni E., Satz A., Eyermann C., Lundqvist T.
  Bioorg. Med. Chem. Lett. 19:930-936(2009) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY, SUBUNIT.
- Ref.4
  "Design of inhibitors of Helicobacter pylori glutamate racemase as selective antibacterial agents: incorporation of imidazoles onto a core pyrazolopyrimidinedione scaffold to improve bioavailabilty."
  Basarab G.S., Hill P., Eyermann C.J., Gowravaram M., Kack H., Osimoni E.
  Bioorg. Med. Chem. Lett. 22:5600-5607(2012) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY.
EC:
5.1.1.3
- Search proteins in UniProtKB for this EC number.
- See the description of this EC number in ENZYME.
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00258
3 Publications
Manual assertion based on experiment inⁱ
- Ref.2
  "Exploitation of structural and regulatory diversity in glutamate racemases."
  Lundqvist T., Fisher S.L., Kern G., Folmer R.H., Xue Y., Newton D.T., Keating T.A., Alm R.A., de Jonge B.L.
  Nature 447:817-822(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY, SUBUNIT, FUNCTION, PATHWAY.
- Ref.3
  "Potent and selective inhibitors of Helicobacter pylori glutamate racemase (MurI): pyridodiazepine amines."
  Geng B., Basarab G., Comita-Prevoir J., Gowravaram M., Hill P., Kiely A., Loch J., MacPherson L., Morningstar M., Mullen G., Osimboni E., Satz A., Eyermann C., Lundqvist T.
  Bioorg. Med. Chem. Lett. 19:930-936(2009) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.87 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY, SUBUNIT.
- Ref.4
  "Design of inhibitors of Helicobacter pylori glutamate racemase as selective antibacterial agents: incorporation of imidazoles onto a core pyrazolopyrimidinedione scaffold to improve bioavailabilty."
  Basarab G.S., Hill P., Eyermann C.J., Gowravaram M., Kack H., Osimoni E.
  Bioorg. Med. Chem. Lett. 22:5600-5607(2012) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY.
Source:
Rhea
Search for this reaction in UniProtKB.
See the description of this reaction in Rhea.
. Show »« Hide
L-glutamate
zoom
=
D-glutamate
zoom

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	70	Proton donor/acceptorUniRule annotationBy similarity		1
Active siteⁱ	181	Proton donor/acceptorUniRule annotationBy similarity		1

GO - Molecular functionⁱ

Complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall organization Source: UniProtKB-KW
peptidoglycan biosynthetic process Source: UniProtKB-UniRule
regulation of cell shape Source: UniProtKB-KW

Complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Isomerase
Biological process	Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCycⁱ	HPYL85963:G1G1A-540-MONOMER
BRENDAⁱ	5.1.1.3, 2604
UniPathwayⁱ	UPA00219

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Glutamate racemaseUniRule annotation (EC:5.1.1.3UniRule annotation3 Publications)
Gene namesⁱ	Name:murIUniRule annotation Synonyms:glr Ordered Locus Names:jhp_0496
Organismⁱ	Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifierⁱ	85963 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori
Proteomesⁱ	UP000000804 Componentⁱ: Chromosome

Pathology & Biotechⁱ

Chemistry databases

ChEMBLⁱ	CHEMBL5185
Drug and drug target database More...DrugBankⁱ	DB08698, 1-[(3S)-5-PHENYL-3-THIOPHEN-2-YL-3H-1,4-BENZODIAZEPIN-2-YL]AZETIDIN-3-OL

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000095477	1 – 255	Glutamate racemaseAdd BLAST		255

Interactionⁱ

Subunit structureⁱ

Homodimer.

2 Publications

Binary interactionsⁱ

Q9ZLT0

With	#Exp.	IntAct
itself	4	EBI-15642574,EBI-15642574

GO - Molecular functionⁱ

identical protein binding
Source: IntAct
Inferred from physical interactionⁱ
- Ref.2
  "Exploitation of structural and regulatory diversity in glutamate racemases."
  Lundqvist T., Fisher S.L., Kern G., Folmer R.H., Xue Y., Newton D.T., Keating T.A., Alm R.A., de Jonge B.L.
  Nature 447:817-822(2007) [PubMed] [Europe PMC] [Abstract]
  Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, CATALYTIC ACTIVITY, SUBUNIT, FUNCTION, PATHWAY.

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-60299N
STRINGⁱ	85963.jhp_0496

Chemistry databases

BindingDBⁱ

Q9ZLT0

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	2 – 10	Combined sources	9
Helixⁱ	13 – 21	Combined sources	9
Beta strandⁱ	26 – 32	Combined sources	7
Turnⁱ	34 – 36	Combined sources	3
Helixⁱ	44 – 58	Combined sources	15
Helixⁱ	59 – 61	Combined sources	3
Beta strandⁱ	66 – 68	Combined sources	3
Helixⁱ	71 – 76	Combined sources	6
Helixⁱ	78 – 84	Combined sources	7
Beta strandⁱ	89 – 93	Combined sources	5
Helixⁱ	94 – 103	Combined sources	10
Beta strandⁱ	111 – 115	Combined sources	5
Helixⁱ	117 – 122	Combined sources	6
Helixⁱ	124 – 131	Combined sources	8
Beta strandⁱ	137 – 141	Combined sources	5
Helixⁱ	145 – 150	Combined sources	6
Helixⁱ	157 – 167	Combined sources	11
Beta strandⁱ	175 – 181	Combined sources	7
Helixⁱ	184 – 187	Combined sources	4
Helixⁱ	188 – 198	Combined sources	11
Beta strandⁱ	206 – 209	Combined sources	4
Helixⁱ	210 – 221	Combined sources	12
Helixⁱ	223 – 225	Combined sources	3
Beta strandⁱ	233 – 240	Combined sources	8
Helixⁱ	242 – 253	Combined sources	12

Show more details

3D structure databases

SMRⁱ	Q9ZLT0
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q9ZLT0

EvolutionaryTraceⁱ

Q9ZLT0

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	7 – 8	Substrate bindingUniRule annotationCombined sources3 Publications	2
Regionⁱ	39 – 40	Substrate bindingUniRule annotationCombined sources3 Publications	2
Regionⁱ	71 – 72	Substrate bindingUniRule annotationCombined sources3 Publications	2
Regionⁱ	182 – 183	Substrate bindingUniRule annotationCombined sources3 Publications	2

Sequence similaritiesⁱ

Belongs to the aspartate/glutamate racemases family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	COG0796, Bacteria
OMAⁱ	LDFFKPH

Family and domain databases

HAMAPⁱ	MF_00258, Glu_racemase, 1 hit
InterProⁱ	View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004391, Glu_race
PANTHERⁱ	PTHR21198:SF2, PTHR21198:SF2, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01177, Asp_Glu_race, 1 hit
SUPFAMⁱ	SSF53681, SSF53681, 2 hits
TIGRFAMsⁱ	TIGR00067, glut_race, 1 hit
PROSITEⁱ	View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9ZLT0-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKIGVFDSGV GGFSVLKSLL KARLFDEIIY YGDSARVPYG TKDPTTIKQF 
        60         70         80         90        100
GLEALDFFKP HEIELLIVAC NTASALALEE MQKYSKIPIV GVIEPSILAI 
       110        120        130        140        150
KRQVEDKNAP ILVLGTKATI QSNAYDNALK QQGYLNISHL ATSLFVPLIE 
       160        170        180        190        200
ESILEGELLE TCMHYYFTPL EILPEVIILG CTHFPLIAQK IEGYFMGHFA 
       210        220        230        240        250
LPTPPLLIHS GDAIVEYLQQ KYALKNNACT FPKVEFHASG DVIWLERQAK 

EWLKL

Length:255

Mass (Da):28,503

Last modified:May 1, 1999 - v1

Checksum:ⁱB31F72E86EBAAC7B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE001439 Genomic DNA Translation: AAD06074.1
PIRⁱ	C71924
NCBI Reference Sequences More...RefSeqⁱ	WP_000690367.1, NZ_CP011330.1

Genome annotation databases

EnsemblBacteriaⁱ	AAD06074; AAD06074; jhp_0496
KEGGⁱ	hpj:jhp_0496
PATRICⁱ	fig\|85963.30.peg.500

Protein	Similar proteins		Species	Score	Length	Source
Q9ZLT0	Glutamate racemase		HELPX		255	UniRef100_Q9ZLT0

Protein	Similar proteins		Species	Score	Length	Source
Q9ZLT0	Glutamate racemase		HELPY		255	UniRef90_Q9ZLT0
Glutamate racemase		HELP2		255
Glutamate racemase		HELPG		255
Glutamate racemase		HELPH		255
Glutamate racemase		HELPS		255
+1278

Protein	Similar proteins		Species	Score	Length	Source
Q9ZLT0	Glutamate racemase		HELP2		255	UniRef50_Q9ZLT0
Glutamate racemase		HELPY		255
Glutamate racemase		HELPS		255
Glutamate racemase		HELPH		255
Glutamate racemase		HELPG		255
+1586

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AE001439 Genomic DNA Translation: AAD06074.1
PIRⁱ	C71924
RefSeqⁱ	WP_000690367.1, NZ_CP011330.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2JFX	X-ray	2.30	A/B	1-255	[»]
	2JFY	X-ray	1.90	A/B	1-255	[»]
	2JFZ	X-ray	1.86	A/B	1-255	[»]
	2W4I	X-ray	1.87	A/B/E/F	1-255	[»]
	4B1F	X-ray	2.05	A/B	1-255	[»]
SMRⁱ	Q9ZLT0
ModBaseⁱ	Search...
PDBe-KBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-60299N
STRINGⁱ	85963.jhp_0496

Chemistry databases

BindingDBⁱ	Q9ZLT0
ChEMBLⁱ	CHEMBL5185
DrugBankⁱ	DB08698, 1-[(3S)-5-PHENYL-3-THIOPHEN-2-YL-3H-1,4-BENZODIAZEPIN-2-YL]AZETIDIN-3-OL

Genome annotation databases

EnsemblBacteriaⁱ	AAD06074; AAD06074; jhp_0496
KEGGⁱ	hpj:jhp_0496
PATRICⁱ	fig\|85963.30.peg.500

Phylogenomic databases

eggNOGⁱ	COG0796, Bacteria
OMAⁱ	LDFFKPH

Enzyme and pathway databases

UniPathwayⁱ	UPA00219
BioCycⁱ	HPYL85963:G1G1A-540-MONOMER
BRENDAⁱ	5.1.1.3, 2604

Miscellaneous databases

EvolutionaryTraceⁱ	Q9ZLT0

EvolutionaryTraceⁱ

Q9ZLT0

Family and domain databases

HAMAPⁱ	MF_00258, Glu_racemase, 1 hit
InterProⁱ	View protein in InterPro IPR015942, Asp/Glu/hydantoin_racemase IPR001920, Asp/Glu_race IPR018187, Asp/Glu_racemase_AS_1 IPR033134, Asp/Glu_racemase_AS_2 IPR004391, Glu_race
PANTHERⁱ	PTHR21198:SF2, PTHR21198:SF2, 1 hit
Pfamⁱ	View protein in Pfam PF01177, Asp_Glu_race, 1 hit
SUPFAMⁱ	SSF53681, SSF53681, 2 hits
TIGRFAMsⁱ	TIGR00067, glut_race, 1 hit
PROSITEⁱ	View protein in PROSITE PS00923, ASP_GLU_RACEMASE_1, 1 hit PS00924, ASP_GLU_RACEMASE_2, 1 hit
ProtoNetⁱ	Search...
MobiDBⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MURI_HELPJ
Accessionⁱ	Q9ZLT0Primary (citable) accession number: Q9ZLT0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
	Last sequence update:	May 1, 1999
	Last modified:	December 2, 2020
	This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9ZLT0 (MURI_HELPJ)

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Glutamate racemase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Q9ZLT0

GO - Molecular functioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Catalytic activityⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ