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Protein

Bifunctional NAD(P)H-hydrate repair enzyme Nnr

Gene

nnr

Organism
Helicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration (By similarity).By similarity

Catalytic activityi

ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH.
ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide.
(6R)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate.

Cofactori

K+By similarityNote: Binds 1 potassium ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57PotassiumBy similarity1
Metal bindingi118PotassiumBy similarity1
Binding sitei149NAD(P)HX (for epimerase activity)By similarity1
Metal bindingi152PotassiumBy similarity1
Binding sitei298NAD(P)HX (for dehydratase activity); via amide nitrogenBy similarity1
Binding sitei412NAD(P)HX (for dehydratase activity)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi383 – 387ADPBy similarity5
Nucleotide bindingi402 – 411ADPBy similarity10

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme
LigandATP-binding, Metal-binding, NAD, NADP, Nucleotide-binding, Potassium

Enzyme and pathway databases

BioCyciHPYL85963:G1G1A-1376-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional NAD(P)H-hydrate repair enzyme Nnr
Alternative name(s):
Nicotinamide nucleotide repair protein
Including the following 2 domains:
ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC:4.2.1.136)
Alternative name(s):
ADP-dependent NAD(P)HX dehydratase
NAD(P)H-hydrate epimerase (EC:5.1.99.6)
Alternative name(s):
NAD(P)HX epimerase
Gene namesi
Name:nnr
Ordered Locus Names:jhp_1281
OrganismiHelicobacter pylori (strain J99 / ATCC 700824) (Campylobacter pylori J99)
Taxonomic identifieri85963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000804 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001190451 – 464Bifunctional NAD(P)H-hydrate repair enzyme NnrAdd BLAST464

Interactioni

Protein-protein interaction databases

STRINGi85963.jhp1281

Structurei

3D structure databases

ProteinModelPortaliQ9ZJM4
SMRiQ9ZJM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 205YjeF N-terminalAdd BLAST198
Domaini213 – 464YjeF C-terminalAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 205NAD(P)H-hydrate epimeraseBy similarityAdd BLAST205
Regioni56 – 60NAD(P)HX (for epimerase activity)By similarity5
Regioni122 – 128NAD(P)HX (for epimerase activity)By similarity7
Regioni213 – 464ADP-dependent (S)-NAD(P)H-hydrate dehydrataseBy similarityAdd BLAST252
Regioni340 – 346NAD(P)HX (for dehydratase activity)By similarity7

Sequence similaritiesi

In the N-terminal section; belongs to the NnrE/AIBP family.Curated
In the C-terminal section; belongs to the NnrD/CARKD family.Curated

Phylogenomic databases

eggNOGiENOG4108ENG Bacteria
COG0062 LUCA
COG0063 LUCA
KOiK17758
K17759
OMAiKSPMCQL
OrthoDBiPOG091H01XZ

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZJM4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSVYEKGNA LDKRVLEEWL LSEDILMENA AMALERAVLQ NASLGAKVII
60 70 80 90 100
LCGSGDNGGD GYTLARRLVG RFKTLVFEMK LAKSPMCQLQ KERAKKVGVV
110 120 130 140 150
IKAWEEKNED LECDVLVDCV VGSAFKGGLE PFLDFESLSQ KARFKIACDI
160 170 180 190 200
PSGIDSKGRV DKRAFKADTT ISMGAIKSCL LSDKAKDYIG ELKVGHLGVF
210 220 230 240 250
NQIYEIPTDT FLLEKSDLKL PLRDRKNAHK GDYGHAHVLL GKHSGAGLLS
260 270 280 290 300
ALSALSFGSG VVSIQALECE ITSNNKPLEL VFCENFPKKL SAFALGMGLE
310 320 330 340 350
NIPKDFKKWL ELAPCVLDAG VFYHKEVLQA LEKEVILTPH PKEFLSLLKS
360 370 380 390 400
VGINISMLEL LDNKLEIARD FSQKYPKVVL LLKGANTLIA HQGRVFINNL
410 420 430 440 450
GSVALAKAGS GDVLAGLIVS LLSQNYTPLD AAINASLAHA LAGLEFKNHY
460
ALTPLDLIEK IKRL
Length:464
Mass (Da):50,677
Last modified:May 1, 1999 - v1
Checksum:i4C43852339A4B52E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06866.1
PIRiG71825
RefSeqiWP_000953985.1, NZ_CP011330.1

Genome annotation databases

EnsemblBacteriaiAAD06866; AAD06866; jhp_1281
KEGGihpj:jhp_1281
PATRICifig|85963.30.peg.1287

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001439 Genomic DNA Translation: AAD06866.1
PIRiG71825
RefSeqiWP_000953985.1, NZ_CP011330.1

3D structure databases

ProteinModelPortaliQ9ZJM4
SMRiQ9ZJM4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi85963.jhp1281

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD06866; AAD06866; jhp_1281
KEGGihpj:jhp_1281
PATRICifig|85963.30.peg.1287

Phylogenomic databases

eggNOGiENOG4108ENG Bacteria
COG0062 LUCA
COG0063 LUCA
KOiK17758
K17759
OMAiKSPMCQL
OrthoDBiPOG091H01XZ

Enzyme and pathway databases

BioCyciHPYL85963:G1G1A-1376-MONOMER

Family and domain databases

CDDicd01171 YXKO-related, 1 hit
Gene3Di3.40.1190.20, 1 hit
3.40.50.10260, 1 hit
HAMAPiMF_01965 NADHX_dehydratase, 1 hit
MF_01966 NADHX_epimerase, 1 hit
InterProiView protein in InterPro
IPR017953 Carbohydrate_kinase_pred_CS
IPR000631 CARKD
IPR030677 Nnr
IPR029056 Ribokinase-like
IPR004443 YjeF_N_dom
IPR036652 YjeF_N_dom_sf
PfamiView protein in Pfam
PF01256 Carb_kinase, 1 hit
PF03853 YjeF_N, 1 hit
PIRSFiPIRSF017184 Nnr, 1 hit
SUPFAMiSSF53613 SSF53613, 1 hit
SSF64153 SSF64153, 1 hit
TIGRFAMsiTIGR00196 yjeF_cterm, 1 hit
TIGR00197 yjeF_nterm, 1 hit
PROSITEiView protein in PROSITE
PS01050 YJEF_C_2, 1 hit
PS51383 YJEF_C_3, 1 hit
PS51385 YJEF_N, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNNR_HELPJ
AccessioniPrimary (citable) accession number: Q9ZJM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: October 10, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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