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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Mycobacterium smegmatis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi318Magnesium or manganese 1UniRule annotation1
Metal bindingi330Magnesium or manganese 1UniRule annotation1
Metal bindingi330Magnesium or manganese 2UniRule annotation1
Metal bindingi332Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi184 – 239ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.3.2.4 3512
UniPathwayi
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
OrganismiMycobacterium smegmatis
Taxonomic identifieri1772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycolicibacterium

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001778431 – 373D-alanine--D-alanine ligaseAdd BLAST373

Structurei

3D structure databases

ProteinModelPortaliQ9ZGN0
SMRiQ9ZGN0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini156 – 363ATP-graspUniRule annotationAdd BLAST208

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
KOiK01921

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZGN0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAPNHPPGR TRVAVVYGGR SSEHAISCVS AGSILRNLDP ERFEVVAIGI
60 70 80 90 100
TPDGSWVLTD GRPETLAITD GKLPAVTEAS GTELALPAAP NRSGQLLALG
110 120 130 140 150
NGPGEILAAV DVVFPVLHGP YGEDGTIQGL LELAGVPYVG SGVLASAAGM
160 170 180 190 200
DKEYTKKLLA AEGLPIGDQV VLRPGVETLD LEQRERLGLP VFVKPARGGS
210 220 230 240 250
SIGVSRVTAW DELPAAVALA RRHDPKVIVE AAVIGRELEC GVLEFPDGRL
260 270 280 290 300
EASTVGEIRV AGVRGREDGF YDFATKYLED AAELDVPAKV DDDVADEIRQ
310 320 330 340 350
LAVRAFTAIG CQGLARVDFF LTDDGPVINE INTMPGFTTI SMYPRMWAAG
360 370
GIDYPTLLAA MVDTAIARGT GLR
Length:373
Mass (Da):39,232
Last modified:May 1, 1999 - v1
Checksum:iFE7151D858C39D43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077728 Genomic DNA Translation: AAC99396.1
RefSeqiWP_011728314.1, NZ_LN831039.1

Genome annotation databases

KEGGimsh:LI98_11915
msn:LI99_11910

Similar proteinsi

Entry informationi

Entry nameiDDL_MYCSM
AccessioniPrimary (citable) accession number: Q9ZGN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 20, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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