UniProtKB - Q9ZF99 (MDH_AQUAR)
Protein
Malate dehydrogenase
Gene
mdh
Organism
Aquaspirillum arcticum
Status
Functioni
Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation
Catalytic activityi
- EC:1.1.1.37UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 95 | SubstrateUniRule annotation | 1 | |
Binding sitei | 101 | SubstrateUniRule annotation | 1 | |
Binding sitei | 108 | NADUniRule annotation | 1 | |
Binding sitei | 115 | NADUniRule annotation1 Publication | 1 | |
Binding sitei | 134 | SubstrateUniRule annotation | 1 | |
Binding sitei | 165 | SubstrateUniRule annotation1 Publication | 1 | |
Active sitei | 190 | Proton acceptorUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 18 | NADUniRule annotation1 Publication | 7 | |
Nucleotide bindingi | 132 – 134 | NADUniRule annotation1 Publication | 3 |
GO - Molecular functioni
- L-malate dehydrogenase activity Source: UniProtKB-UniRule
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- malate metabolic process Source: InterPro
- tricarboxylic acid cycle Source: UniProtKB-UniRule
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Tricarboxylic acid cycle |
Ligand | NAD |
Enzyme and pathway databases
BRENDAi | 1.1.1.37, 395 |
SABIO-RKi | Q9ZF99 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mdhUniRule annotation |
Organismi | Aquaspirillum arcticum |
Taxonomic identifieri | 87645 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Chromobacteriaceae › Aquaspirillum |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000113346 | 2 – 329 | Malate dehydrogenaseAdd BLAST | 328 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9ZF99 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9ZF99 |
Family & Domainsi
Sequence similaritiesi
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
HAMAPi | MF_01517, Malate_dehydrog_2, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR010945, Malate_DH_type2 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR23382, PTHR23382, 1 hit |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01759, MalateDH-SF1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9ZF99-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKTPMRVAV TGAAGQICYS LLFRIANGDM LGKDQPVILQ LLEIPNEKAQ
60 70 80 90 100
KALQGVMMEI DDCAFPLLAG MTAHADPMTA FKDADVALLV GARPRGPGME
110 120 130 140 150
RKDLLEANAQ IFTVQGKAID AVASRNIKVL VVGNPANTNA YIAMKSAPSL
160 170 180 190 200
PAKNFTAMLR LDHNRALSQI AAKTGKPVSS IEKLFVWGNH SPTMYADYRY
210 220 230 240 250
AQIDGASVKD MINDDAWNRD TFLPTVGKRG AAIIDARGVS SAASAANAAI
260 270 280 290 300
DHIHDWVLGT AGKWTTMGIP SDGSYGIPEG VIFGFPVTTE NGEYKIVQGL
310 320
SIDAFSQERI NVTLNELLEE QNGVQHLLG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109682 Genomic DNA Translation: AAD13225.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF109682 Genomic DNA Translation: AAD13225.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B8P | X-ray | 1.90 | A | 1-329 | [»] | |
1B8U | X-ray | 2.50 | A | 1-329 | [»] | |
1B8V | X-ray | 2.10 | A | 1-329 | [»] | |
SMRi | Q9ZF99 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB02637, Oxaloacetate Ion |
Enzyme and pathway databases
BRENDAi | 1.1.1.37, 395 |
SABIO-RKi | Q9ZF99 |
Miscellaneous databases
EvolutionaryTracei | Q9ZF99 |
Family and domain databases
Gene3Di | 3.90.110.10, 1 hit |
HAMAPi | MF_01517, Malate_dehydrog_2, 1 hit |
InterProi | View protein in InterPro IPR001557, L-lactate/malate_DH IPR022383, Lactate/malate_DH_C IPR001236, Lactate/malate_DH_N IPR015955, Lactate_DH/Glyco_Ohase_4_C IPR010945, Malate_DH_type2 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR23382, PTHR23382, 1 hit |
Pfami | View protein in Pfam PF02866, Ldh_1_C, 1 hit PF00056, Ldh_1_N, 1 hit |
PIRSFi | PIRSF000102, Lac_mal_DH, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF56327, SSF56327, 1 hit |
TIGRFAMsi | TIGR01759, MalateDH-SF1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MDH_AQUAR | |
Accessioni | Q9ZF99Primary (citable) accession number: Q9ZF99 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 23, 2007 | |
Last modified: | October 7, 2020 | |
This is version 117 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families