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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi146Zinc 1UniRule annotation1
Metal bindingi149Zinc 1UniRule annotation1
Metal bindingi163Zinc 2UniRule annotation1
Metal bindingi166Zinc 2UniRule annotation1
Metal bindingi185Zinc 2UniRule annotation1
Metal bindingi188Zinc 2UniRule annotation1
Metal bindingi199Zinc 1UniRule annotation1
Metal bindingi202Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 211CR-typeUniRule annotationAdd BLAST79

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processDNA replication, Stress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciRPRO272947:G1GT0-187-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:RP184
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000708731 – 370Chaperone protein DnaJAdd BLAST370

Proteomic databases

PRIDEiQ9ZDY0

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi272947.RP184

Structurei

3D structure databases

ProteinModelPortaliQ9ZDY0
SMRiQ9ZDY0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 68JUniRule annotationAdd BLAST65
Repeati146 – 153CXXCXGXG motif8
Repeati163 – 170CXXCXGXG motif8
Repeati185 – 192CXXCXGXG motif8
Repeati199 – 206CXXCXGXG motif8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi75 – 116Gly-richAdd BLAST42

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri133 – 211CR-typeUniRule annotationAdd BLAST79

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105BZ5 Bacteria
COG0484 LUCA
HOGENOMiHOG000226717
KOiK03686
OMAiDMGGFAD

Family and domain databases

CDDicd06257 DnaJ, 1 hit
cd10719 DnaJ_zf, 1 hit
Gene3Di1.10.287.110, 1 hit
HAMAPiMF_01152 DnaJ, 1 hit
InterProiView protein in InterPro
IPR012724 DnaJ
IPR002939 DnaJ_C
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR008971 HSP40/DnaJ_pept-bd
IPR001305 HSP_DnaJ_Cys-rich_dom
IPR036410 HSP_DnaJ_Cys-rich_dom_sf
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF01556 DnaJ_C, 1 hit
PF00684 DnaJ_CXXCXGXG, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
SSF49493 SSF49493, 3 hits
SSF57938 SSF57938, 1 hit
TIGRFAMsiTIGR02349 DnaJ_bact, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit
PS51188 ZF_CR, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZDY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDYYQVLG VSKTASQADI KKAYLKLAKQ YHPDTTNAND AEKKFKEINA
60 70 80 90 100
AYDVLKDEQK RAAYDRFGHD AFQSQQARGG GNNGSFHPDI NDIFGDFFSD
110 120 130 140 150
FMGSGRRKQT SSKIRGSDLK YDLTIKLEEA FHGIEKNISF SSEVKCDTCH
160 170 180 190 200
GTGSEKGETI TTCDACGGVG ATRIQQGFFT LEQTCHKCQG NGQIIKNPCK
210 220 230 240 250
KCHGMGRYHK QRNLSINIPA GVENGTRIRH TGEGEAGIRG GNNGDLYVDI
260 270 280 290 300
AIKPHDIYKV DGANLHCKLP ISFVHAALGG EIEVPVIEGG KVKLTIPAGT
310 320 330 340 350
QNGDQLRLRS KGMSKVRSTI RGDMLTHIHV EVPKNLSKKQ RELLEEFKKE
360 370
SINEKENDSS FFNKMKSMWS
Length:370
Mass (Da):41,013
Last modified:May 1, 1999 - v1
Checksum:iAE6721285B4F6186
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235270 Genomic DNA Translation: CAA14650.1
PIRiC71729
RefSeqiNP_220573.1, NC_000963.1
WP_004595923.1, NC_000963.1

Genome annotation databases

EnsemblBacteriaiCAA14650; CAA14650; CAA14650
GeneIDi883882
KEGGirpr:RP184
PATRICifig|272947.5.peg.191

Similar proteinsi

Entry informationi

Entry nameiDNAJ_RICPR
AccessioniPrimary (citable) accession number: Q9ZDY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

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