Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aconitate hydratase A

Gene

acnA

Organism
Rickettsia prowazekii (strain Madrid E)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the catabolism of short chain fatty acids (SCFA) via the tricarboxylic acid (TCA)(acetyl degradation route) and probably the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate. Could catalyze the hydration of 2-methyl-cis-aconitate to yield (2R,3S)-2-methylisocitrate. The apo form of AcnA functions as a RNA-binding regulatory protein.By similarity

Catalytic activityi

Citrate = isocitrate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.By similarity
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. Aconitate hydratase A (acnA)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Pathwayi: propanoate degradation

This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.By similarity
View all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi426Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi492Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi495Iron-sulfur (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase, RNA-binding
Biological processTricarboxylic acid cycle
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciRPRO272947:G1GT0-803-MONOMER
UniPathwayi
UPA00223;UER00718

UPA00946

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase ABy similarity (EC:4.2.1.3By similarity)
Short name:
ACNBy similarity
Short name:
AconitaseBy similarity
Alternative name(s):
(2R,3S)-2-methylisocitrate dehydrataseBy similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydrataseBy similarity
Iron-responsive protein-likeBy similarity
Short name:
IRP-likeBy similarity
Probable 2-methyl-cis-aconitate hydrataseBy similarity (EC:4.2.1.99By similarity)
RNA-binding proteinBy similarity
Gene namesi
Name:acnA
Ordered Locus Names:RP799
OrganismiRickettsia prowazekii (strain Madrid E)
Taxonomic identifieri272947 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiatyphus group
Proteomesi
  • UP000002480 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000766651 – 878Aconitate hydratase AAdd BLAST878

Proteomic databases

PRIDEiQ9ZCF4

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi272947.RP799

Structurei

3D structure databases

ProteinModelPortaliQ9ZCF4
SMRiQ9ZCF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Phylogenomic databases

eggNOGiENOG4108I0Z Bacteria
COG1048 LUCA
HOGENOMiHOG000025703
KOiK01681
OMAiMRIIPPG

Family and domain databases

Gene3Di3.20.19.10, 1 hit
3.30.499.10, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR006249 Aconitase/IRP2
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PANTHERiPTHR11670 PTHR11670, 1 hit
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01341 aconitase_1, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ZCF4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKVHNSEYI KELVVDNTSY KIYDINKAAS DIGLPLKKLP YSLRVLLENV
60 70 80 90 100
LRTNGNKENL LVFKEWLKTK KSNTEIDFMP ARVLMQDFTG VPAIVDLAAM
110 120 130 140 150
RDAMQKIGCN PLKINPLIPV DLVIDHSVSV DSYGNKESFD QNVHMEMKRN
160 170 180 190 200
IERYQFLKWG QQAFNNFKVV PPGTGICHQV NLEFLSQVVW HNDGTAYPDS
210 220 230 240 250
LVGTDSHTTM VNGLSVLGWG VGGIEAESAM LGQPITMIIP EVIGVKLIGK
260 270 280 290 300
LAGMATATDL VLTITEILRR KKVVGKFVEF FGNGLRNLTI SDRATISNMS
310 320 330 340 350
PEYGATCGFF PIDQETLKYL EVTGREITQI KLVEKYAIEQ NLWYNCEDTQ
360 370 380 390 400
EYTEVLELDL STVYSSLAGP KRPQDRVNLN CVASNFQNEL PYFALANKDI
410 420 430 440 450
DKKYAVANQN YAIGNGDVVI AAITSCTNTS NPSVMIGAAL LAKKALEHGL
460 470 480 490 500
NVKPWVKTSL APGSKVVTEY LKLSGLDKYL DALGFNLVGY GCTTCIGNSG
510 520 530 540 550
SLNPEIENTI NKNRLVVASV LSGNRNFEGR INPLTKASYL ASPILVVAYA
560 570 580 590 600
LSGTLNIDLT NTPIGANIYL KDIWPSQKEI DEVIANSINS SMFIEKYADV
610 620 630 640 650
FNGTKEWRDL QITTGTNYNW DKNSTYINNP PYFDNIGSEH SIKDIKSARI
660 670 680 690 700
LAIFGDSITT DHISPAGSIS KNSPAAKYLI KHHIEPLDFN SYGSRRGNHE
710 720 730 740 750
VMMRGTFANI RIKNEMCNGV EGGFTINQLS GVQQTIYDTA MDYKAHDIPL
760 770 780 790 800
VIFAGKEYGS GSSRDWAAKG PGLLGIKAVI AESFERIHRS NLVGMGILPL
810 820 830 840 850
TFTGKNTRLN LKLDGSEIID LIGLSENIKP YNLVKCVIKK QTNEISTIDL
860 870
ILQIFTENEI NYIKHGSIMQ FVVESLKG
Length:878
Mass (Da):97,120
Last modified:May 1, 1999 - v1
Checksum:i2835539115E66805
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA Translation: CAA15225.1
PIRiA71641
RefSeqiNP_221149.1, NC_000963.1
WP_004596907.1, NC_000963.1

Genome annotation databases

EnsemblBacteriaiCAA15225; CAA15225; CAA15225
GeneIDi883812
KEGGirpr:RP799
PATRICifig|272947.5.peg.835

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ235273 Genomic DNA Translation: CAA15225.1
PIRiA71641
RefSeqiNP_221149.1, NC_000963.1
WP_004596907.1, NC_000963.1

3D structure databases

ProteinModelPortaliQ9ZCF4
SMRiQ9ZCF4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272947.RP799

Proteomic databases

PRIDEiQ9ZCF4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAA15225; CAA15225; CAA15225
GeneIDi883812
KEGGirpr:RP799
PATRICifig|272947.5.peg.835

Phylogenomic databases

eggNOGiENOG4108I0Z Bacteria
COG1048 LUCA
HOGENOMiHOG000025703
KOiK01681
OMAiMRIIPPG

Enzyme and pathway databases

UniPathwayi
UPA00223;UER00718

UPA00946

BioCyciRPRO272947:G1GT0-803-MONOMER

Family and domain databases

Gene3Di3.20.19.10, 1 hit
3.30.499.10, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR006249 Aconitase/IRP2
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PANTHERiPTHR11670 PTHR11670, 1 hit
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01341 aconitase_1, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACNA_RICPR
AccessioniPrimary (citable) accession number: Q9ZCF4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Rickettsia prowazekii
    Rickettsia prowazekii (strain Madrid E): entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again