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UniProtKB - Q9ZB63 (CARY_GEOSE)
Protein
Carbamoyl-phosphate synthase arginine-specific large chain
Gene
carB
Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Functioni
Catalytic activityi
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphateEC:6.3.5.5
Cofactori
Temperature dependencei
Thermostable up to 63 degrees Celsius.
: L-arginine biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate. This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 285 | Magnesium 1By similarity | 1 | |
Metal bindingi | 299 | Magnesium 1By similarity | 1 | |
Metal bindingi | 299 | Magnesium 2By similarity | 1 | |
Metal bindingi | 301 | Magnesium 2By similarity | 1 | |
Metal bindingi | 822 | Magnesium 3By similarity | 1 | |
Metal bindingi | 834 | Magnesium 3By similarity | 1 | |
Metal bindingi | 834 | Magnesium 4By similarity | 1 | |
Metal bindingi | 836 | Magnesium 4By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 160 – 217 | ATPBy similarityAdd BLAST | 58 | |
Nucleotide bindingi | 702 – 756 | ATPBy similarityAdd BLAST | 55 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- arginine biosynthetic process Source: UniProtKB-UniRule
- pyrimidine nucleotide biosynthetic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Amino-acid biosynthesis, Arginine biosynthesis |
Ligand | ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
SABIO-RKi | Q9ZB63 |
UniPathwayi | UPA00068;UER00171 |
Names & Taxonomyi
Protein namesi | Recommended name: Carbamoyl-phosphate synthase arginine-specific large chain (EC:6.3.5.5)Alternative name(s): Carbamoyl-phosphate synthetase ammonia chain |
Gene namesi | Name:carB |
Organismi | Geobacillus stearothermophilus (Bacillus stearothermophilus) |
Taxonomic identifieri | 1422 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Geobacillus |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144990 | 1 – 1043 | Carbamoyl-phosphate synthase arginine-specific large chainAdd BLAST | 1043 |
Interactioni
Subunit structurei
Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 134 – 328 | ATP-grasp 1Add BLAST | 195 | |
Domaini | 676 – 863 | ATP-grasp 2Add BLAST | 188 | |
Domaini | 930 – 1037 | MGS-likePROSITE-ProRule annotationAdd BLAST | 108 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 402 | Carboxyphosphate synthetic domainAdd BLAST | 402 | |
Regioni | 403 – 550 | Oligomerization domainAdd BLAST | 148 | |
Regioni | 551 – 932 | Carbamoyl phosphate synthetic domainAdd BLAST | 382 | |
Regioni | 933 – 1043 | Allosteric domainAdd BLAST | 111 |
Sequence similaritiesi
Belongs to the CarB family.Curated
Keywords - Domaini
RepeatFamily and domain databases
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit |
HAMAPi | MF_01210_B, CPSase_L_chain_B, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR011607, MGS-like_dom IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit |
PRINTSi | PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF52440, SSF52440, 2 hits |
TIGRFAMsi | TIGR01369, CPSaseII_lrg, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS51855, MGS, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9ZB63-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPKDSSLQSI LLIGSGRSSS AKAAEFDYSG TQACIAFKEE GYRVILVNNN
60 70 80 90 100
PATIMTDDVH ADAVYFEPLT VEAVEAIIAK ETPDGLLATF GGQTGLNLAF
110 120 130 140 150
QLHEAGVLKK YGVRLLGTPI EAIKRPRGGP RTFRALMHEL GEPVPESEIV
160 170 180 190 200
TSVEEAVAFA EQIGFPIIIR PAYTLGGTGG GIAENMEQFL ALVEKGLNES
210 220 230 240 250
PIHQCLIERS VAGFKEIEYE VMRDQSNTCI TVCNMENVDP VGIHTGDSIV
260 270 280 290 300
VAPSQTLTDE EYQMLRSSAV KIISALGIIG GCNIQFALDP NSKQYYLIEV
310 320 330 340 350
NPRVSRSSAL ASKATGYPIA ALPAKLAVGY TLAELVNPVT KTTYASFEPA
360 370 380 390 400
LDYVVVKFPR LPFDKFPHAD RKLGTQMKAT GEVMAIDRNM ERAFQKAVQS
410 420 430 440 450
LEGKNNGLLL PELSVKTNDE LKQLLVDKDD RRFFAILELL RRGVAVEAIH
460 470 480 490 500
KWTKIDRFFL CSFERLVALE KQAAAATLDT IEEQTFRFLK EKGCSDAFLA
510 520 530 540 550
ETWGVTELDV RNKRKELGIV PSYKMVDTCA AEFHSETDYY YSTYFGEDER
560 570 580 590 600
KQPSGKEKVL IIGAGPIRIG QGIEFDYSSV HSVFALQKEG YETVMINNNP
610 620 630 640 650
ETVSTDFAVA DRLYFEPLTL ESVLDVIEAE QIKHVIVQFG GQTAINLVKG
660 670 680 690 700
LEEAGVPLLG VTYDMIDQLE DRDRFYQLLE ELDIPHVPGL VANNAEELAA
710 720 730 740 750
KAAEIGYPVL LRPSYVIGGC GMFIVHSEAQ LAALIEQGEL TYPILIDAYL
760 770 780 790 800
DGKEAEADIV TDGTDIVLPV IIEHVEKAGV HSGDSYAWLP AQTLTGEEKA
810 820 830 840 850
KIIDYAGRIA KKLGFKGIMN IQYVIADGNV YVLEVNPRAS RTVPIVSKTT
860 870 880 890 900
GVPLAQIATK LLLGKSLVDI VDEKARGLAV MPYAVLKYPV FSTHKLPGVD
910 920 930 940 950
PMVGPEMKST GEGISIAATK EEAAYKAFYP YLQKKANANE VYVIGNIDAE
960 970 980 990 1000
LEAEMTAKQL TIVADVPFSD WVKRDTALAV IDLGKEEGEA NKRMTALSRQ
1010 1020 1030 1040
LLVFTERETL KLFLQALDVD HLDVQPIHGW LEKKKQAEQA VIA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43091 Genomic DNA Translation: AAC78719.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U43091 Genomic DNA Translation: AAC78719.1 |
3D structure databases
AlphaFoldDBi | Q9ZB63 |
SMRi | Q9ZB63 |
ModBasei | Search... |
Enzyme and pathway databases
UniPathwayi | UPA00068;UER00171 |
SABIO-RKi | Q9ZB63 |
Family and domain databases
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit |
HAMAPi | MF_01210_B, CPSase_L_chain_B, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR011607, MGS-like_dom IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit |
PRINTSi | PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF52440, SSF52440, 2 hits |
TIGRFAMsi | TIGR01369, CPSaseII_lrg, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS51855, MGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CARY_GEOSE | |
Accessioni | Q9ZB63Primary (citable) accession number: Q9ZB63 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families