UniProtKB - Q9Z315 (SNUT1_MOUSE)
Protein
U4/U6.U5 tri-snRNP-associated protein 1
Gene
Sart1
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a role in mRNA splicing as a component of the U4/U6-U5 tri-snRNP, one of the building blocks of the spliceosome. May also bind to DNA. Appears to play a role in hypoxia-induced regulation of EPO gene expression.1 Publication
GO - Biological processi
- maturation of 5S rRNA Source: GO_Central
- mRNA cis splicing, via spliceosome Source: GO_Central
- mRNA splicing, via spliceosome Source: MGI
- positive regulation of cytotoxic T cell differentiation Source: MGI
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Names & Taxonomyi
Protein namesi | Recommended name: U4/U6.U5 tri-snRNP-associated protein 1Alternative name(s): Hypoxia-associated factor Squamous cell carcinoma antigen recognized by T-cells 1 Short name: SART-1 Short name: mSART-1 |
Gene namesi | Name:Sart1 Synonyms:Haf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1309453, Sart1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- Cajal body Source: MGI
- catalytic step 2 spliceosome Source: MGI
- nuclear speck Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- U2-type precatalytic spliceosome Source: MGI
- U4/U6 x U5 tri-snRNP complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000223309 | 1 – 806 | U4/U6.U5 tri-snRNP-associated protein 1Add BLAST | 806 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 125 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 133 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 141 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 141 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 147 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 188 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 189 | PhosphothreonineCombined sources | 1 | |
Cross-linki | 277 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 321 | PhosphoserineBy similarity | 1 | |
Cross-linki | 329 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 336 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 348 | PhosphoserineBy similarity | 1 | |
Cross-linki | 400 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 414 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 430 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 480 | PhosphoserineCombined sources | 1 | |
Modified residuei | 492 | PhosphoserineBy similarity | 1 | |
Modified residuei | 527 | PhosphoserineBy similarity | 1 | |
Cross-linki | 554 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 597 | PhosphoserineBy similarity | 1 | |
Modified residuei | 602 | PhosphoserineBy similarity | 1 | |
Modified residuei | 604 | PhosphoserineBy similarity | 1 | |
Modified residuei | 627 | PhosphoserineBy similarity | 1 | |
Cross-linki | 654 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 663 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 690 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 701 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 705 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 715 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 729 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 755 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 764 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 767 | PhosphoserineBy similarity | 1 | |
Modified residuei | 770 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 781 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 786 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 795 | PhosphoserineBy similarity | 1 | |
Cross-linki | 797 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Post-translational modificationi
Sumoylated with SUMO2.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Z315 |
jPOSTi | Q9Z315 |
MaxQBi | Q9Z315 |
PaxDbi | Q9Z315 |
PeptideAtlasi | Q9Z315 |
PRIDEi | Q9Z315 |
PTM databases
iPTMneti | Q9Z315 |
PhosphoSitePlusi | Q9Z315 |
Expressioni
Tissue specificityi
Ubiquitously expressed. Shows a high expression in fetal liver and a low expression in adult liver.1 Publication
Developmental stagei
Expressed at maximal level at 12 dpc. Declines progressively until birth.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000039148, Expressed in ear and 324 other tissues |
ExpressionAtlasi | Q9Z315, baseline and differential |
Genevisiblei | Q9Z315, MM |
Interactioni
Subunit structurei
Identified in the spliceosome C complex.
Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 203075, 25 interactors |
IntActi | Q9Z315, 26 interactors |
MINTi | Q9Z315 |
STRINGi | 10090.ENSMUSP00000047397 |
Miscellaneous databases
RNActi | Q9Z315, protein |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 157 – 231 | Sequence analysisAdd BLAST | 75 | |
Coiled coili | 494 – 540 | Sequence analysisAdd BLAST | 47 |
Sequence similaritiesi
Belongs to the SNU66/SART1 family.Curated
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG2217, Eukaryota |
GeneTreei | ENSGT00390000007071 |
HOGENOMi | CLU_009379_3_0_1 |
InParanoidi | Q9Z315 |
OMAi | ARWENKQ |
OrthoDBi | 1068640at2759 |
PhylomeDBi | Q9Z315 |
TreeFami | TF318828 |
Family and domain databases
InterProi | View protein in InterPro IPR005011, SNU66/SART1 |
PANTHERi | PTHR14152, PTHR14152, 1 hit |
Pfami | View protein in Pfam PF03343, SART-1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9Z315-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGSSKKHRGE KEAAGTTAAA GTGGTTEQPP RHREHKKHKH RSSGGGSSGG
60 70 80 90 100
ERRKRSRERG SERGSGRRGA EAEARSGAHG RERSQAEPSE RRVKREKRDD
110 120 130 140 150
GYEAAASSKA SSGDASSLSI EETNKLRAKL GLKPLEVNAV KKEAGTKEEP
160 170 180 190 200
VAADVINPMA LRQREELREK LAAAKEKRLL NQKLGKIKTL GEDDPWLDDT
210 220 230 240 250
AAWIERSRQL QKEKDLAEKR AKLLEEMDQE FGVSTLVEEE FEQRRQDLYS
260 270 280 290 300
ARDLQGLTVE HAIDSFREGE TVVLTLKDKG VLQDGEDVLV NVNMVDKERA
310 320 330 340 350
DKNVELRKKK PDYLPYAEDE SVDDLAQQKP RSILAKYDEE LEGERPHSFR
360 370 380 390 400
LEQGGMADGL RERELEEIRT KLRLQAQSLS SVGPRLASEY LSPEEMVTFK
410 420 430 440 450
KTKRRVKKIR KKEKEVIMRA DDLLPLGDQT QDGDFGSRLR GRGRRRVPEV
460 470 480 490 500
EEEALEDEEK DPVAQPPPSD DTRVENMDIS DEEDGGALPP GSPEGLEEDE
510 520 530 540 550
AELELQKQLE KGRRLRQLQQ LQQLRDSGEK VLEIVKKLES RQRGWEEEED
560 570 580 590 600
PERKGTIVFN ATSEFCRTLG EIPTYGLAGN REEQEELMDF ERDEERSANG
610 620 630 640 650
GSESDGEENI GWSTVNLDEE KQHQDFSASS TTILDEEPIV NRGLAAALLL
660 670 680 690 700
CQNKGLLETT VQKVARVKAP NKSLPSAVYC IEDKMAIDDK YSRREEYRGF
710 720 730 740 750
TQDFKEKDGY KPDVKIEYVD ETGRKLTPKE AFRQLSHRFH GKGSGKMKTE
760 770 780 790 800
RRMKKLDEEA LLKKMSSSDT PLGTVALLQE KQKAQKTPYI VLSGSGKSMN
ANTITK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A494B9E9 | A0A494B9E9_MOUSE | U4/U6.U5 tri-snRNP-associated prote... | Sart1 | 113 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | A → T in AAD20645 (PubMed:10887110).Curated | 1 | |
Sequence conflicti | 70 | A → T in AAH28823 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 70 | A → T in AAH51394 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 566 | C → Y in AAD20645 (PubMed:10887110).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014721 mRNA Translation: BAA36583.1 AF129931 mRNA Translation: AAD20645.1 BC028823 mRNA Translation: AAH28823.1 BC051394 mRNA Translation: AAH51394.1 AF105334 Genomic DNA Translation: AAD38450.1 |
CCDSi | CCDS29460.1 |
RefSeqi | NP_058578.3, NM_016882.3 |
Genome annotation databases
Ensembli | ENSMUST00000044207; ENSMUSP00000047397; ENSMUSG00000039148 |
GeneIDi | 20227 |
KEGGi | mmu:20227 |
UCSCi | uc008gcu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB014721 mRNA Translation: BAA36583.1 AF129931 mRNA Translation: AAD20645.1 BC028823 mRNA Translation: AAH28823.1 BC051394 mRNA Translation: AAH51394.1 AF105334 Genomic DNA Translation: AAD38450.1 |
CCDSi | CCDS29460.1 |
RefSeqi | NP_058578.3, NM_016882.3 |
3D structure databases
SMRi | Q9Z315 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 203075, 25 interactors |
IntActi | Q9Z315, 26 interactors |
MINTi | Q9Z315 |
STRINGi | 10090.ENSMUSP00000047397 |
PTM databases
iPTMneti | Q9Z315 |
PhosphoSitePlusi | Q9Z315 |
Proteomic databases
EPDi | Q9Z315 |
jPOSTi | Q9Z315 |
MaxQBi | Q9Z315 |
PaxDbi | Q9Z315 |
PeptideAtlasi | Q9Z315 |
PRIDEi | Q9Z315 |
Protocols and materials databases
Antibodypediai | 30019, 207 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000044207; ENSMUSP00000047397; ENSMUSG00000039148 |
GeneIDi | 20227 |
KEGGi | mmu:20227 |
UCSCi | uc008gcu.1, mouse |
Organism-specific databases
CTDi | 9092 |
MGIi | MGI:1309453, Sart1 |
Phylogenomic databases
eggNOGi | KOG2217, Eukaryota |
GeneTreei | ENSGT00390000007071 |
HOGENOMi | CLU_009379_3_0_1 |
InParanoidi | Q9Z315 |
OMAi | ARWENKQ |
OrthoDBi | 1068640at2759 |
PhylomeDBi | Q9Z315 |
TreeFami | TF318828 |
Enzyme and pathway databases
Reactomei | R-MMU-72163, mRNA Splicing - Major Pathway |
Miscellaneous databases
BioGRID-ORCSi | 20227, 8 hits in 17 CRISPR screens |
ChiTaRSi | Sart1, mouse |
PROi | PR:Q9Z315 |
RNActi | Q9Z315, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039148, Expressed in ear and 324 other tissues |
ExpressionAtlasi | Q9Z315, baseline and differential |
Genevisiblei | Q9Z315, MM |
Family and domain databases
InterProi | View protein in InterPro IPR005011, SNU66/SART1 |
PANTHERi | PTHR14152, PTHR14152, 1 hit |
Pfami | View protein in Pfam PF03343, SART-1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SNUT1_MOUSE | |
Accessioni | Q9Z315Primary (citable) accession number: Q9Z315 Secondary accession number(s): Q8K155, Q9R1I9, Q9R270 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | May 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families