UniProtKB - Q9Z311 (MECR_RAT)
Enoyl-[acyl-carrier-protein] reductase, mitochondrial
Mecr
Functioni
Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II). Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Displays a preference for medium-chain over short- and long-chain substrates (By similarity).
May provide the octanoyl chain used for lipoic acid biosynthesis, regulating protein lipoylation and mitochondrial respiratory activity particularly in Purkinje cells (By similarity).
By similarityCaution
Catalytic activityi
- EC:1.3.1.104By similarityThis reaction proceeds in the backwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 94 | Proton donorBy similarity | 1 | |
Binding sitei | 167 | NADPBy similarity | 1 | |
Binding sitei | 368 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 193 – 196 | NADPBy similarity | 4 | |
Nucleotide bindingi | 216 – 218 | NADPBy similarity | 3 | |
Nucleotide bindingi | 285 – 288 | NADPBy similarity | 4 | |
Nucleotide bindingi | 310 – 312 | NADPBy similarity | 3 |
GO - Molecular functioni
- signaling receptor binding Source: RGD
- trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB
GO - Biological processi
- fatty acid biosynthetic process Source: UniProtKB-KW
- fatty acid metabolic process Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Ligand | NADP |
Enzyme and pathway databases
Reactomei | R-RNO-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Names & Taxonomyi
Protein namesi | Recommended name: Enoyl-[acyl-carrier-protein] reductase, mitochondrial (EC:1.3.1.104By similarity)Alternative name(s): 2-enoyl thioester reductase1 Publication Nuclear receptor-binding factor 1 Short name: NRBF-1 |
Gene namesi | Name:Mecr Synonyms:Nrbf1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 3208, Mecr |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 53 | MitochondrionSequence analysisAdd BLAST | 53 | |
ChainiPRO_0000000890 | 54 – 373 | Enoyl-[acyl-carrier-protein] reductase, mitochondrialAdd BLAST | 320 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 61 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 61 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 252 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 252 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 267 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 267 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 316 | N6-succinyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q9Z311 |
PTM databases
iPTMneti | Q9Z311 |
PhosphoSitePlusi | Q9Z311 |
Interactioni
Subunit structurei
Homodimer (By similarity).
Isoform 2 interacts with PPARA in the nucleus and increases its activity (PubMed:25031892).
By similarity1 PublicationGO - Molecular functioni
- signaling receptor binding Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031375 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0025, Eukaryota |
InParanoidi | Q9Z311 |
OrthoDBi | 1269870at2759 |
PhylomeDBi | Q9Z311 |
TreeFami | TF312886 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLVSRRLTGA RARAPLLASL LEAWCRQGRT TSSYSAFSEP SHVRALVYGN
60 70 80 90 100
HGDPAKVIQL KNLELTAVEG SDVHVKMLAA PINPSDINMI QGNYGLLPKL
110 120 130 140 150
PAVGGNEGVG QVIAVGSSVS GLKPGDWVIP ANAGLGTWRT EAVFSEEALI
160 170 180 190 200
GVPKDIPLQS AATLGVNPCT AYRMLVDFEQ LQPGDSVIQN ASNSGVGQAV
210 220 230 240 250
IQIASALGLK TINVIRDRPD IKKLTDRLKD LGADYVLTEE ELRMPETKNI
260 270 280 290 300
FKDLPLPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVTASVSML
310 320 330 340 350
IFKDLKLRGF WLSQWKKNHS PDEFKELILI LCNLIRQGQL TAPAWSGIPL
360 370
QDYQQALEAS MKPFVSLKQI LTM
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140TAE6 | A0A140TAE6_RAT | Enoyl-[acyl-carrier-protein] reduct... | Mecr | 373 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_057305 | 1 – 76 | Missing in isoform 2. Add BLAST | 76 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015724 mRNA Translation: BAA34804.1 AABR06040190 Genomic DNA No translation available. AABR06040191 Genomic DNA No translation available. |
RefSeqi | NP_058905.1, NM_017209.1 [Q9Z311-1] |
Genome annotation databases
GeneIDi | 29470 |
KEGGi | rno:29470 |
UCSCi | RGD:3208, rat [Q9Z311-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB015724 mRNA Translation: BAA34804.1 AABR06040190 Genomic DNA No translation available. AABR06040191 Genomic DNA No translation available. |
RefSeqi | NP_058905.1, NM_017209.1 [Q9Z311-1] |
3D structure databases
AlphaFoldDBi | Q9Z311 |
SMRi | Q9Z311 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000031375 |
PTM databases
iPTMneti | Q9Z311 |
PhosphoSitePlusi | Q9Z311 |
Proteomic databases
PaxDbi | Q9Z311 |
Genome annotation databases
GeneIDi | 29470 |
KEGGi | rno:29470 |
UCSCi | RGD:3208, rat [Q9Z311-1] |
Organism-specific databases
CTDi | 51102 |
RGDi | 3208, Mecr |
Phylogenomic databases
eggNOGi | KOG0025, Eukaryota |
InParanoidi | Q9Z311 |
OrthoDBi | 1269870at2759 |
PhylomeDBi | Q9Z311 |
TreeFami | TF312886 |
Enzyme and pathway databases
Reactomei | R-RNO-77346, Beta oxidation of decanoyl-CoA to octanoyl-CoA-CoA |
Miscellaneous databases
PROi | PR:Q9Z311 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH-like_C IPR013154, ADH_N IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 1 hit SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MECR_RAT | |
Accessioni | Q9Z311Primary (citable) accession number: Q9Z311 Secondary accession number(s): F1LPY7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | May 1, 1999 | |
Last modified: | May 25, 2022 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families