UniProtKB - Q9Z309 (CIB2_MOUSE)
Protein
Calcium and integrin-binding family member 2
Gene
Cib2
Organism
Mus musculus (Mouse)
Status
Functioni
Calcium-binding protein critical for proper photoreceptor cell maintenance and function. Plays a role in intracellular calcium homeostasis by decreasing ATP-induced calcium release (PubMed:23023331). May be involved in the mechanotransduction process (By similarity).By similarity1 Publication
Miscellaneous
The binding of either calcium or magnesium significantly increases the structural stability of the protein in comparison to apo-CIB (calcium- and magnesium-free form).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 116 – 127 | 1PROSITE-ProRule annotationAdd BLAST | 12 | |
Calcium bindingi | 157 – 168 | 2PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: MGI
- integrin binding Source: MGI
- magnesium ion binding Source: UniProtKB
- protein-containing complex binding Source: MGI
- protein homodimerization activity Source: UniProtKB
GO - Biological processi
- calcium ion homeostasis Source: UniProtKB
- cellular response to ATP Source: UniProtKB
- photoreceptor cell maintenance Source: UniProtKB
- positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Keywordsi
Ligand | Calcium, Magnesium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium and integrin-binding family member 2Alternative name(s): Kinase-interacting protein 2 Short name: KIP 2 |
Gene namesi | Name:Cib2 Synonyms:Kip2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1929293, Cib2 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma membrane
- sarcolemma 1 Publication
Other locations
- stereocilium 1 Publication
- Photoreceptor inner segment 1 Publication
- photoreceptor outer segment 1 Publication
Note: Colocalized with ITGA7 at the myotendinous junctions (MTJ) and at the neuromuscular junctions (NMJ) (PubMed:18611855). Localizes in the cuticular plate along and at the tip of the stereocilia of vestibular sensory hair cells (By similarity).By similarity2 Publications
Cytoskeleton
- cuticular plate Source: UniProtKB
Plasma Membrane
- sarcolemma Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- muscle tendon junction Source: MGI
- neuromuscular junction Source: MGI
- photoreceptor inner segment Source: UniProtKB
- photoreceptor outer segment Source: UniProtKB
- stereocilium Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000073535 | 1 – 187 | Calcium and integrin-binding family member 2Add BLAST | 187 |
Proteomic databases
PaxDbi | Q9Z309 |
PRIDEi | Q9Z309 |
ProteomicsDBi | 279079 |
PTM databases
PhosphoSitePlusi | Q9Z309 |
Expressioni
Tissue specificityi
Expressed in the supporting cells in both the organ of Corti and the vestibular sensory epithelia. Expressed in inner and outer segments of photoreceptor cells, as well as in the pigmented epithelium. Also observed in the inner and outer plexiform layers and in the ganglion cell layer (at protein level) (PubMed:23023331). Widely expressed (PubMed:23023331). Strongly expressed in skeletal muscles, brain, kidney and liver (PubMed:23023331). Also expressed in the skeletal muscle, retina and cochlea (PubMed:18611855, PubMed:23023331). Expressed in megakaryocytes and endothelial cells (PubMed:18989529).3 Publications
Developmental stagei
Expressed in the central nervous system and in muscle at 16 dpc, onward (PubMed:18611855). Expressed in the inner ear and retina at 15.5 and 17.5 dpc (PubMed:23023331).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000037493, Expressed in brown adipose tissue and 219 other tissues |
ExpressionAtlasi | Q9Z309, baseline and differential |
Genevisiblei | Q9Z309, MM |
Interactioni
Subunit structurei
Homodimer (By similarity).
Interacts with WHRN and MYO7A (By similarity).
Interacts with ITGA2B (via C-terminus cytoplasmic tail region); this interaction is stabilized/increased in a calcium and magnesium-dependent manner (PubMed:18611855).
Interacts with ITGA7 (via C-terminus cytoplasmic tail region); this interaction is stabilized/increased in a calcium and magnesium-dependent manner (PubMed:18989529).
By similarity2 PublicationsGO - Molecular functioni
- integrin binding Source: MGI
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038527 |
Miscellaneous databases
RNActi | Q9Z309, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 66 – 101 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 103 – 138 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 144 – 179 | EF-hand 3PROSITE-ProRule annotationAdd BLAST | 36 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0038, Eukaryota |
GeneTreei | ENSGT00940000157327 |
HOGENOMi | CLU_061288_6_0_1 |
InParanoidi | Q9Z309 |
OMAi | SYVEFEH |
OrthoDBi | 1271942at2759 |
PhylomeDBi | Q9Z309 |
TreeFami | TF313865 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits |
i Sequence
Sequence statusi: Complete.
Q9Z309-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNKQTIFTE EQLDNYQDCT FFNKKDILKL HARFYELAPN LVPMDYRKSP
60 70 80 90 100
IVHVPMSLII QMPELRENPF KERIVEAFSE DGEGNLTFND FVDMFSVLCE
110 120 130 140 150
SAPRELKANY AFKIYDFNTD NFICKEDLEM TLARLTKSEL EEDEVVLVCD
160 170 180
KVIEEADLDG DGKLGFADFE DMIAKAPDFL STFHIRI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016080 mRNA Translation: BAA36545.1 BC005739 mRNA Translation: AAH05739.1 |
CCDSi | CCDS40642.1 |
RefSeqi | NP_062660.1, NM_019686.5 |
Genome annotation databases
Ensembli | ENSMUST00000041901; ENSMUSP00000038527; ENSMUSG00000037493 |
GeneIDi | 56506 |
KEGGi | mmu:56506 |
UCSCi | uc009prf.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB016080 mRNA Translation: BAA36545.1 BC005739 mRNA Translation: AAH05739.1 |
CCDSi | CCDS40642.1 |
RefSeqi | NP_062660.1, NM_019686.5 |
3D structure databases
SMRi | Q9Z309 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038527 |
PTM databases
PhosphoSitePlusi | Q9Z309 |
Proteomic databases
PaxDbi | Q9Z309 |
PRIDEi | Q9Z309 |
ProteomicsDBi | 279079 |
Protocols and materials databases
Antibodypediai | 14987, 202 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000041901; ENSMUSP00000038527; ENSMUSG00000037493 |
GeneIDi | 56506 |
KEGGi | mmu:56506 |
UCSCi | uc009prf.1, mouse |
Organism-specific databases
CTDi | 10518 |
MGIi | MGI:1929293, Cib2 |
Phylogenomic databases
eggNOGi | KOG0038, Eukaryota |
GeneTreei | ENSGT00940000157327 |
HOGENOMi | CLU_061288_6_0_1 |
InParanoidi | Q9Z309 |
OMAi | SYVEFEH |
OrthoDBi | 1271942at2759 |
PhylomeDBi | Q9Z309 |
TreeFami | TF313865 |
Miscellaneous databases
BioGRID-ORCSi | 56506, 0 hits in 52 CRISPR screens |
ChiTaRSi | Cib2, mouse |
PROi | PR:Q9Z309 |
RNActi | Q9Z309, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000037493, Expressed in brown adipose tissue and 219 other tissues |
ExpressionAtlasi | Q9Z309, baseline and differential |
Genevisiblei | Q9Z309, MM |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom |
Pfami | View protein in Pfam PF13499, EF-hand_7, 1 hit |
SMARTi | View protein in SMART SM00054, EFh, 3 hits |
SUPFAMi | SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 2 hits PS50222, EF_HAND_2, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CIB2_MOUSE | |
Accessioni | Q9Z309Primary (citable) accession number: Q9Z309 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | May 1, 1999 | |
Last modified: | April 7, 2021 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot