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Protein

Klotho

Gene

Kl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).By similarity
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.By similarity

Catalytic activityi

A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Names & Taxonomyi

Protein namesi
Recommended name:
Klotho (EC:3.2.1.31)
Cleaved into the following chain:
Gene namesi
Name:Kl
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620396 Kl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini35 – 983ExtracellularSequence analysisAdd BLAST949
Transmembranei984 – 1004HelicalSequence analysisAdd BLAST21
Topological domaini1005 – 1014CytoplasmicSequence analysis10

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Sequence analysisAdd BLAST34
ChainiPRO_000004224935 – 1014KlothoAdd BLAST980
ChainiPRO_000004225035 – ?Klotho peptideBy similarity

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi161N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi285N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi346N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi609N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi614N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi696N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9Z2Y9
PRIDEiQ9Z2Y9

Expressioni

Tissue specificityi

Present in cortical renal tubules and the parathyroid (at protein level). Strongly expressed in kidney. Expressed at low levels in brain, lung, intestine and ovaries.3 Publications

Developmental stagei

Expressed faintly from E18 in the kidney. Expression increases in the kidney after 4 days of age.1 Publication

Inductioni

Down-regulated by angiotensin II and iron overload (at protein level). Down-regulated by acute inflammatory stress, and in models for long-term hypertension, diabetes mellitus and chronic renal failure.5 Publications

Gene expression databases

GenevisibleiQ9Z2Y9 RN

Interactioni

Subunit structurei

Homodimer. Interacts with FGF23 and FGFR1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001449

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Y9
SMRiQ9Z2Y9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 508Glycosyl hydrolase-1 1Add BLAST450
Regioni517 – 955Glycosyl hydrolase-1 2Add BLAST439

Domaini

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
HOGENOMiHOG000060126
HOVERGENiHBG081856
InParanoidiQ9Z2Y9
KOiK14756
OrthoDBiEOG091G0035
PhylomeDBiQ9Z2Y9
TreeFamiTF314803

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
IPR028546 Klotho
PANTHERiPTHR10353 PTHR10353, 5 hits
PTHR10353:SF10 PTHR10353:SF10, 5 hits
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 3 hits
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9Z2Y9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPARAPPRRL PRLLLLRLLS LHLLLLTLRA RCLSAEPGQG AQTWARFARP
60 70 80 90 100
PVPEASGLLH DTFPDGFLWA VGSAAYQTEG GWRQHGKGAS IWDTFTHHPR
110 120 130 140 150
AIPEDSPIVM APSGAPLPPL PSTGDVASDS YNNVYRDTEG LRELGVTHYR
160 170 180 190 200
FSISWARVLP NGTAGTPNRE GLRYYRRLLE RLRELGVQPV VTLYHWDLPQ
210 220 230 240 250
RLQDTYGGWA NRALADHFRD YAELCFRHFG GQVKYWITID NPYVVAWHGY
260 270 280 290 300
ATGRLAPGVR GSSRLGYLVA HNLLLAHAKV WRLYNTSFRP TQGGRVSIAL
310 320 330 340 350
GSHWITPRRM TDYHIRECQK SLDFVLGWFA KPIFIDGDYP KSMKNNLSSL
360 370 380 390 400
LPDFTESEKR FIRGTADFFA LSFGPTLSFQ LLDPSMKFRQ LESPSLRQLL
410 420 430 440 450
SWIDLEYNHP QIFIVENGWF VSGTTRRDDA KYMYYLKKFI MESLKAIRLD
460 470 480 490 500
GVDVIGYTAW SLMDGFEWHR GYSIRRGLFY VDFLSQDKEL LPKSSALFYQ
510 520 530 540 550
KLIENNGFPP LPENQPLEGT FPCDFAWGVV DNYIQVDPTL SQFTDPNVYL
560 570 580 590 600
WDVHHSKRLI KVDGVVAKKR KPYCVDFSAI RPQITLLREM RVTHFRFSLD
610 620 630 640 650
WALILPLGNQ TQVNRTVLHF YRCMVSELVH ANITPVVALW QPATPHQGLP
660 670 680 690 700
HALAKHGAWE NPHTALAFAD YANLCFEELG HWVKFWITIN EPNSRNMTYR
710 720 730 740 750
AGHHLLKAHA LAWHLYDDKF RAAQKGKISI ALQVDWIEPA CPFSQKDKEV
760 770 780 790 800
AERVLEFDVG WLAEPIFGSG DYPHVMREWL NQKNNFLLPY FTEDEKKLIR
810 820 830 840 850
GSFDFLALSH YTTILVDWEK EDPIKYNDYL EVQEMTDITW LNSPNQVAVV
860 870 880 890 900
PWGLRKALNW LRFKYGDLPM FVTANGIDDD PHAEQDSLRM YYIKNYVNEA
910 920 930 940 950
LKAYVLDGIN LCGYFAYSLS DRSVPKSGFY RYAANQFEPK PSIKHYRKII
960 970 980 990 1000
DNNGFLGSGT LGRFCPEEYT VCTGCGFFQT RKSLLAFISF LVFAFVTSLA
1010
LIYYYSKKGR RRYK
Length:1,014
Mass (Da):116,800
Last modified:May 1, 1999 - v1
Checksum:i16F3B57581AC1DF0
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0H2UH96A0A0H2UH96_RAT
Klotho
Kl
905Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017820 mRNA Translation: BAA34740.1
PIRiJE0333
RefSeqiNP_112626.1, NM_031336.1
UniGeneiRn.30061

Genome annotation databases

GeneIDi83504
KEGGirno:83504
UCSCiRGD:620396 rat

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

The thread of life - Issue 65 of December 2005

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017820 mRNA Translation: BAA34740.1
PIRiJE0333
RefSeqiNP_112626.1, NM_031336.1
UniGeneiRn.30061

3D structure databases

ProteinModelPortaliQ9Z2Y9
SMRiQ9Z2Y9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001449

Protein family/group databases

CAZyiGH1 Glycoside Hydrolase Family 1

Proteomic databases

PaxDbiQ9Z2Y9
PRIDEiQ9Z2Y9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83504
KEGGirno:83504
UCSCiRGD:620396 rat

Organism-specific databases

CTDi9365
RGDi620396 Kl

Phylogenomic databases

eggNOGiKOG0626 Eukaryota
COG2723 LUCA
HOGENOMiHOG000060126
HOVERGENiHBG081856
InParanoidiQ9Z2Y9
KOiK14756
OrthoDBiEOG091G0035
PhylomeDBiQ9Z2Y9
TreeFamiTF314803

Miscellaneous databases

PROiPR:Q9Z2Y9

Gene expression databases

GenevisibleiQ9Z2Y9 RN

Family and domain databases

InterProiView protein in InterPro
IPR001360 Glyco_hydro_1
IPR033132 Glyco_hydro_1_N_CS
IPR017853 Glycoside_hydrolase_SF
IPR028546 Klotho
PANTHERiPTHR10353 PTHR10353, 5 hits
PTHR10353:SF10 PTHR10353:SF10, 5 hits
PfamiView protein in Pfam
PF00232 Glyco_hydro_1, 3 hits
PRINTSiPR00131 GLHYDRLASE1
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS00653 GLYCOSYL_HYDROL_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKLOT_RAT
AccessioniPrimary (citable) accession number: Q9Z2Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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