Psmd10

Functionⁱ

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.By similarity1 Publication

Acts as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export (By similarity).By similarity

GO - Molecular functionⁱ

transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

apoptotic process Source: UniProtKB-KW
cytoplasmic sequestering of NF-kappaB Source: UniProtKB
negative regulation of apoptotic process Source: UniProtKB
negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
negative regulation of MAPK cascade Source: UniProtKB
negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
negative regulation of release of cytochrome c from mitochondria Source: UniProtKB
negative regulation of transcription by RNA polymerase II Source: UniProtKB
positive regulation of cell growth Source: UniProtKB
positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
positive regulation of protein ubiquitination Source: UniProtKB
proteasome regulatory particle assembly Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Chaperone
Biological process	Apoptosis

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 26S proteasome non-ATPase regulatory subunit 10 Alternative name(s): 26S proteasome regulatory subunit p28 Gankyrin
Gene namesⁱ	Name:Psmd10
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome X

Organism-specific databases

MGIⁱ	MGI:1858898 Psmd10

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000067046	1 – 231	26S proteasome non-ATPase regulatory subunit 10Add BLAST		231

Proteomic databases

EPDⁱ	Q9Z2X2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9Z2X2
PaxDbⁱ	Q9Z2X2
PeptideAtlas More...PeptideAtlasⁱ	Q9Z2X2
PRIDEⁱ	Q9Z2X2

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	Q9Z2X2

REPRODUCTION-2DPAGEⁱ

Q9Z2X2

PTM databases

iPTMnetⁱ	Q9Z2X2
PhosphoSitePlusⁱ	Q9Z2X2

Expressionⁱ

Inductionⁱ

Up-regulated by activated HRAS.1 Publication

Gene expression databases

Bgeeⁱ	ENSMUSG00000031429
ExpressionAtlasⁱ	Q9Z2X2 baseline and differential
Genevisibleⁱ	Q9Z2X2 MM

Interactionⁱ

Subunit structureⁱ

Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome. Stays associated throughout the assembly of the PA700/19S RC and is released upon association with the 20S core. Interacts with PSMC4. Interacts with RB1. Interacts with CDK4. Interacts with MDM2. Interacts with RELA. Associates with a CDK4:CCND2 serine/threonine kinase complex (By similarity). Interacts with ARHGDIA and increases the interaction between ARHGDIA and RHOA, hence promotes ARHGDIA inactivation of RHOA and ROCK (By similarity).By similarity

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
PSMC4	P43686	4	EBI-8377084,EBI-743997	From Homo sapiens.
RB1	P06400	3	EBI-8377084,EBI-491274	From Homo sapiens.

Show more details

GO - Molecular functionⁱ

transcription factor binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	207302, 4 interactors
Database of interacting proteins More...DIPⁱ	DIP-29273N
IntActⁱ	Q9Z2X2, 4 interactors
Molecular INTeraction database More...MINTⁱ	Q9Z2X2
STRINGⁱ	10090.ENSMUSP00000033805

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	6 – 8	Combined sources	3
Helixⁱ	9 – 16	Combined sources	8
Helixⁱ	19 – 28	Combined sources	10
Helixⁱ	30 – 34	Combined sources	5
Helixⁱ	43 – 50	Combined sources	8
Helixⁱ	53 – 61	Combined sources	9
Helixⁱ	76 – 83	Combined sources	8
Helixⁱ	86 – 94	Combined sources	9
Helixⁱ	109 – 115	Combined sources	7
Helixⁱ	119 – 127	Combined sources	9
Helixⁱ	142 – 149	Combined sources	8
Helixⁱ	152 – 160	Combined sources	9
Helixⁱ	175 – 181	Combined sources	7
Helixⁱ	185 – 193	Combined sources	9
Helixⁱ	208 – 211	Combined sources	4
Helixⁱ	214 – 229	Combined sources	16

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2DVW	X-ray	2.30	A	1-231	[»]
	2DWZ	X-ray	2.40	A/C	1-231	[»]
	3AJI	X-ray	2.05	A/C	1-231	[»]
ProteinModelPortalⁱ	Q9Z2X2
SMRⁱ	Q9Z2X2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q9Z2X2

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Repeatⁱ	3 – 36	ANK 1Add BLAST	34
Repeatⁱ	37 – 69	ANK 2Add BLAST	33
Repeatⁱ	70 – 102	ANK 3Add BLAST	33
Repeatⁱ	103 – 135	ANK 4Add BLAST	33
Repeatⁱ	136 – 168	ANK 5Add BLAST	33
Repeatⁱ	169 – 201	ANK 6Add BLAST	33
Repeatⁱ	202 – 226	ANK 7Add BLAST	25

Keywords - Domainⁱ

ANK repeat, Repeat

Phylogenomic databases

eggNOGⁱ	KOG4412 Eukaryota COG0666 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144039
HOGENOMⁱ	HOG000158359
HOVERGENⁱ	HBG053737
InParanoidⁱ	Q9Z2X2
KEGG Orthology (KO) More...KOⁱ	K06694
OMAⁱ	SVNQNGC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0KEZ

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00023 Ank, 1 hit PF12796 Ank_2, 2 hits
PRINTSⁱ	PR01415 ANKYRIN
SMARTⁱ	View protein in SMART SM00248 ANK, 5 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 5 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9Z2X2-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEGCVSNIMI CNLAYSGKLD ELKERILADK SLATRTDQDS RTALHWACSA 
        60         70         80         90        100
GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLVKGAHVN 
       110        120        130        140        150
AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYDA TAMHRAAAKG 
       160        170        180        190        200
NLKMVHILLF YKASTNIQDT EGNTPLHLAC DEERVEEAKF LVTQGASIYI 
       210        220        230 
ENKEEKTPLQ VAKGGLGLIL KRLAESEEAS M

Length:231

Mass (Da):25,084

Last modified:January 9, 2013 - v3

Checksum:ⁱBCE7B9A79C91358B

GO

Sequence cautionⁱ

The sequence BAB31128 differs from that shown. Reason: Frameshift at position 218.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	101	A → S in BAA36969 (Ref. 1) Curated	1
Sequence conflictⁱ	122	A → S in BAA36969 (Ref. 1) Curated	1
Sequence conflictⁱ	226	S → G in BAB26053 (PubMed:16141072).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB022022 mRNA Translation: BAA36969.1 AK009068 mRNA Translation: BAB26053.1 AK018233 mRNA Translation: BAB31128.1 Frameshift. AK136400 mRNA Translation: BAE22964.1 AL672306 Genomic DNA Translation: CAM15395.1 CH466616 Genomic DNA Translation: EDL23939.1 BC026931 mRNA Translation: AAH26931.1 BC056196 mRNA Translation: AAH56196.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30444.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001157649.1, NM_001164177.1 NP_058579.2, NM_016883.4
UniGeneⁱ	Mm.17640

Genome annotation databases

Ensemblⁱ	ENSMUST00000033805; ENSMUSP00000033805; ENSMUSG00000031429 ENSMUST00000230246; ENSMUSP00000155318; ENSMUSG00000116430
GeneIDⁱ	53380
KEGGⁱ	mmu:53380
UCSC genome browser More...UCSCⁱ	uc009ulj.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length
Q9Z2X2	UPI000719F4FC		EQUAS		270
UPI0003C12292		PANHO		260
UPI00084789C6		CAPHI		260
26S proteasome non-ATPase regulatory subunit 10		PTEAL		259
UPI0007879A98		ROUAE		259
+24

Protein	Similar proteins		Species	Score	Length
Q9Z2X2	UPI000719F4FC		EQUAS		270
UPI00084789C6		CAPHI		260
UPI0003C12292		PANHO		260
26S proteasome non-ATPase regulatory subunit 10		PTEAL		259
UPI0007879A98		ROUAE		259
+33

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB022022 mRNA Translation: BAA36969.1 AK009068 mRNA Translation: BAB26053.1 AK018233 mRNA Translation: BAB31128.1 Frameshift. AK136400 mRNA Translation: BAE22964.1 AL672306 Genomic DNA Translation: CAM15395.1 CH466616 Genomic DNA Translation: EDL23939.1 BC026931 mRNA Translation: AAH26931.1 BC056196 mRNA Translation: AAH56196.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS30444.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001157649.1, NM_001164177.1 NP_058579.2, NM_016883.4
UniGeneⁱ	Mm.17640

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2DVW	X-ray	2.30	A	1-231	[»]
	2DWZ	X-ray	2.40	A/C	1-231	[»]
	3AJI	X-ray	2.05	A/C	1-231	[»]
ProteinModelPortalⁱ	Q9Z2X2
SMRⁱ	Q9Z2X2
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	207302, 4 interactors
Database of interacting proteins More...DIPⁱ	DIP-29273N
IntActⁱ	Q9Z2X2, 4 interactors
Molecular INTeraction database More...MINTⁱ	Q9Z2X2
STRINGⁱ	10090.ENSMUSP00000033805

PTM databases

iPTMnetⁱ	Q9Z2X2
PhosphoSitePlusⁱ	Q9Z2X2

2D gel databases

REPRODUCTION-2DPAGE More...REPRODUCTION-2DPAGEⁱ	Q9Z2X2

REPRODUCTION-2DPAGEⁱ

Q9Z2X2

Proteomic databases

EPDⁱ	Q9Z2X2
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9Z2X2
PaxDbⁱ	Q9Z2X2
PeptideAtlas More...PeptideAtlasⁱ	Q9Z2X2
PRIDEⁱ	Q9Z2X2

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000033805; ENSMUSP00000033805; ENSMUSG00000031429 ENSMUST00000230246; ENSMUSP00000155318; ENSMUSG00000116430
GeneIDⁱ	53380
KEGGⁱ	mmu:53380
UCSC genome browser More...UCSCⁱ	uc009ulj.2 mouse

Organism-specific databases

CTDⁱ	5716
MGIⁱ	MGI:1858898 Psmd10

Phylogenomic databases

eggNOGⁱ	KOG4412 Eukaryota COG0666 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00910000144039
HOGENOMⁱ	HOG000158359
HOVERGENⁱ	HBG053737
InParanoidⁱ	Q9Z2X2
KEGG Orthology (KO) More...KOⁱ	K06694
OMAⁱ	SVNQNGC
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0KEZ

Miscellaneous databases

EvolutionaryTraceⁱ	Q9Z2X2
Protein Ontology More...PROⁱ	PR:Q9Z2X2
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031429
ExpressionAtlasⁱ	Q9Z2X2 baseline and differential
Genevisibleⁱ	Q9Z2X2 MM

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00204 ANK, 1 hit
Gene3Dⁱ	1.25.40.20, 1 hit
InterProⁱ	View protein in InterPro IPR002110 Ankyrin_rpt IPR020683 Ankyrin_rpt-contain_dom IPR036770 Ankyrin_rpt-contain_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00023 Ank, 1 hit PF12796 Ank_2, 2 hits
PRINTSⁱ	PR01415 ANKYRIN
SMARTⁱ	View protein in SMART SM00248 ANK, 5 hits
SUPFAMⁱ	SSF48403 SSF48403, 1 hit
PROSITEⁱ	View protein in PROSITE PS50297 ANK_REP_REGION, 1 hit PS50088 ANK_REPEAT, 5 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PSD10_MOUSE
Accessionⁱ	Q9Z2X2Primary (citable) accession number: Q9Z2X2 Secondary accession number(s): Q8R0G2, Q9D383, Q9D7N8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
	Last sequence update:	January 9, 2013
	Last modified:	June 20, 2018
	This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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Supporting data

UniProtKB - Q9Z2X2 (PSD10_MOUSE)

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26S proteasome non-ATPase regulatory subunit 10

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Cytoskeleton

Cytosol

Nucleus

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ