UniProtKB - Q9Z2X2 (PSD10_MOUSE)
26S proteasome non-ATPase regulatory subunit 10
Psmd10
Functioni
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity).
Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.
By similarity1 PublicationActs as an oncoprotein by being involved in negative regulation of tumor suppressors RB1 and p53/TP53. Overexpression is leading to phosphorylation of RB1 and proteasomal degradation of RB1. Regulates CDK4-mediated phosphorylation of RB1 by competing with CDKN2A for binding with CDK4. Facilitates binding of MDM2 to p53/TP53 and the mono- and polyubiquitination of p53/TP53 by MDM2 suggesting a function in targeting the TP53:MDM2 complex to the 26S proteasome. Involved in p53-independent apoptosis. Involved in regulation of NF-kappa-B by retaining it in the cytoplasm. Binds to the NF-kappa-B component RELA and accelerates its XPO1/CRM1-mediated nuclear export (By similarity).
By similarityGO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cytoplasmic sequestering of NF-kappaB Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
- negative regulation of MAPK cascade Source: UniProtKB
- negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
- negative regulation of release of cytochrome c from mitochondria Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- positive regulation of cell growth Source: UniProtKB
- positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of protein ubiquitination Source: UniProtKB
- proteasome regulatory particle assembly Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Biological process | Apoptosis |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8932339, ROS sensing by NFE2L2 R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Names & Taxonomyi
Protein namesi | Recommended name: 26S proteasome non-ATPase regulatory subunit 10Alternative name(s): 26S proteasome regulatory subunit p28 Gankyrin |
Gene namesi | Name:Psmd10 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1858898, Psmd10 |
VEuPathDBi | HostDB:ENSMUSG00000031429 |
Subcellular locationi
Cytoskeleton
- intermediate filament cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- proteasome complex Source: MGI
- proteasome regulatory particle, base subcomplex Source: Ensembl
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000067046 | 1 – 231 | 26S proteasome non-ATPase regulatory subunit 10Add BLAST | 231 |
Proteomic databases
EPDi | Q9Z2X2 |
MaxQBi | Q9Z2X2 |
PaxDbi | Q9Z2X2 |
PeptideAtlasi | Q9Z2X2 |
PRIDEi | Q9Z2X2 |
ProteomicsDBi | 291829 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z2X2 |
PTM databases
iPTMneti | Q9Z2X2 |
PhosphoSitePlusi | Q9Z2X2 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000031429, Expressed in primary oocyte and 307 other tissues |
ExpressionAtlasi | Q9Z2X2, baseline and differential |
Genevisiblei | Q9Z2X2, MM |
Interactioni
Subunit structurei
Part of transient complex containing PSMD10, PSMC4, PSMC5 and PAAF1 formed during the assembly of the 26S proteasome. Stays associated throughout the assembly of the PA700/19S RC and is released upon association with the 20S core.
Interacts with PSMC4.
Interacts with RB1.
Interacts with CDK4.
Interacts with MDM2.
Interacts with RELA. Associates with a CDK4:CCND2 serine/threonine kinase complex (By similarity).
Interacts with ARHGDIA and increases the interaction between ARHGDIA and RHOA, hence promotes ARHGDIA inactivation of RHOA and ROCK (By similarity).
By similarityBinary interactionsi
Q9Z2X2
With | #Exp. | IntAct |
---|---|---|
PSMC4 [P43686] from Homo sapiens. | 4 | EBI-8377084,EBI-743997 |
RB1 [P06400] from Homo sapiens. | 3 | EBI-8377084,EBI-491274 |
Protein-protein interaction databases
BioGRIDi | 207302, 9 interactors |
DIPi | DIP-29273N |
IntActi | Q9Z2X2, 3 interactors |
MINTi | Q9Z2X2 |
STRINGi | 10090.ENSMUSP00000033805 |
Miscellaneous databases
RNActi | Q9Z2X2, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q9Z2X2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z2X2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 3 – 36 | ANK 1Add BLAST | 34 | |
Repeati | 37 – 69 | ANK 2Add BLAST | 33 | |
Repeati | 70 – 102 | ANK 3Add BLAST | 33 | |
Repeati | 103 – 135 | ANK 4Add BLAST | 33 | |
Repeati | 136 – 168 | ANK 5Add BLAST | 33 | |
Repeati | 169 – 201 | ANK 6Add BLAST | 33 | |
Repeati | 202 – 226 | ANK 7Add BLAST | 25 |
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG4412, Eukaryota |
GeneTreei | ENSGT00940000153404 |
InParanoidi | Q9Z2X2 |
OMAi | WAVAYNR |
OrthoDBi | 1514637at2759 |
PhylomeDBi | Q9Z2X2 |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
IDEALi | IID50037 |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR039334, PSMD10-like |
PANTHERi | PTHR24126:SF24, PTHR24126:SF24, 1 hit |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 5 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MEGCVSNIMI CNLAYSGKLD ELKERILADK SLATRTDQDS RTALHWACSA
60 70 80 90 100
GHTEIVEFLL QLGVPVNDKD DAGWSPLHIA ASAGRDEIVK ALLVKGAHVN
110 120 130 140 150
AVNQNGCTPL HYAASKNRHE IAVMLLEGGA NPDAKDHYDA TAMHRAAAKG
160 170 180 190 200
NLKMVHILLF YKASTNIQDT EGNTPLHLAC DEERVEEAKF LVTQGASIYI
210 220 230
ENKEEKTPLQ VAKGGLGLIL KRLAESEEAS M
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AG83 | A2AG83_MOUSE | 26S proteasome non-ATPase regulator... | Psmd10 | 151 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 101 | A → S in BAA36969 (Ref. 1) Curated | 1 | |
Sequence conflicti | 122 | A → S in BAA36969 (Ref. 1) Curated | 1 | |
Sequence conflicti | 226 | S → G in BAB26053 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB022022 mRNA Translation: BAA36969.1 AK009068 mRNA Translation: BAB26053.1 AK018233 mRNA Translation: BAB31128.1 Frameshift. AK136400 mRNA Translation: BAE22964.1 AL672306 Genomic DNA No translation available. CH466616 Genomic DNA Translation: EDL23939.1 BC026931 mRNA Translation: AAH26931.1 BC056196 mRNA Translation: AAH56196.1 |
CCDSi | CCDS30444.1 |
RefSeqi | NP_001157649.1, NM_001164177.1 NP_058579.2, NM_016883.4 |
Genome annotation databases
Ensembli | ENSMUST00000033805; ENSMUSP00000033805; ENSMUSG00000031429 |
GeneIDi | 53380 |
KEGGi | mmu:53380 |
UCSCi | uc009ulj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB022022 mRNA Translation: BAA36969.1 AK009068 mRNA Translation: BAB26053.1 AK018233 mRNA Translation: BAB31128.1 Frameshift. AK136400 mRNA Translation: BAE22964.1 AL672306 Genomic DNA No translation available. CH466616 Genomic DNA Translation: EDL23939.1 BC026931 mRNA Translation: AAH26931.1 BC056196 mRNA Translation: AAH56196.1 |
CCDSi | CCDS30444.1 |
RefSeqi | NP_001157649.1, NM_001164177.1 NP_058579.2, NM_016883.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DVW | X-ray | 2.30 | A | 1-231 | [»] | |
2DWZ | X-ray | 2.40 | A/C | 1-231 | [»] | |
3AJI | X-ray | 2.05 | A/C | 1-231 | [»] | |
SMRi | Q9Z2X2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 207302, 9 interactors |
DIPi | DIP-29273N |
IntActi | Q9Z2X2, 3 interactors |
MINTi | Q9Z2X2 |
STRINGi | 10090.ENSMUSP00000033805 |
PTM databases
iPTMneti | Q9Z2X2 |
PhosphoSitePlusi | Q9Z2X2 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z2X2 |
Proteomic databases
EPDi | Q9Z2X2 |
MaxQBi | Q9Z2X2 |
PaxDbi | Q9Z2X2 |
PeptideAtlasi | Q9Z2X2 |
PRIDEi | Q9Z2X2 |
ProteomicsDBi | 291829 |
Protocols and materials databases
Antibodypediai | 496, 442 antibodies from 33 providers |
DNASUi | 53380 |
Genome annotation databases
Ensembli | ENSMUST00000033805; ENSMUSP00000033805; ENSMUSG00000031429 |
GeneIDi | 53380 |
KEGGi | mmu:53380 |
UCSCi | uc009ulj.2, mouse |
Organism-specific databases
CTDi | 5716 |
MGIi | MGI:1858898, Psmd10 |
VEuPathDBi | HostDB:ENSMUSG00000031429 |
Phylogenomic databases
eggNOGi | KOG4412, Eukaryota |
GeneTreei | ENSGT00940000153404 |
InParanoidi | Q9Z2X2 |
OMAi | WAVAYNR |
OrthoDBi | 1514637at2759 |
PhylomeDBi | Q9Z2X2 |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174084, Autodegradation of Cdh1 by Cdh1:APC/C R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-187577, SCF(Skp2)-mediated degradation of p27/p21 R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8932339, ROS sensing by NFE2L2 R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8939902, Regulation of RUNX2 expression and activity R-MMU-8941858, Regulation of RUNX3 expression and activity R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 53380, 0 hits in 63 CRISPR screens |
ChiTaRSi | Psmd10, mouse |
EvolutionaryTracei | Q9Z2X2 |
PROi | PR:Q9Z2X2 |
RNActi | Q9Z2X2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031429, Expressed in primary oocyte and 307 other tissues |
ExpressionAtlasi | Q9Z2X2, baseline and differential |
Genevisiblei | Q9Z2X2, MM |
Family and domain databases
Gene3Di | 1.25.40.20, 1 hit |
IDEALi | IID50037 |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf IPR039334, PSMD10-like |
PANTHERi | PTHR24126:SF24, PTHR24126:SF24, 1 hit |
Pfami | View protein in Pfam PF00023, Ank, 1 hit PF12796, Ank_2, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 5 hits |
SUPFAMi | SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 5 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PSD10_MOUSE | |
Accessioni | Q9Z2X2Primary (citable) accession number: Q9Z2X2 Secondary accession number(s): Q8R0G2, Q9D383, Q9D7N8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2002 |
Last sequence update: | January 9, 2013 | |
Last modified: | February 23, 2022 | |
This is version 168 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references