UniProtKB - Q9Z2X1 (HNRPF_MOUSE)
Protein
Heterogeneous nuclear ribonucleoprotein F
Gene
Hnrnpf
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity).By similarity
GO - Molecular functioni
- RNA binding Source: GO_Central
- single-stranded RNA binding Source: UniProtKB
- TBP-class protein binding Source: MGI
- transcription factor binding Source: MGI
GO - Biological processi
- mRNA processing Source: UniProtKB-KW
- regulation of RNA splicing Source: UniProtKB
- RNA splicing Source: UniProtKB-KW
Keywordsi
Molecular function | Ribonucleoprotein, RNA-binding |
Biological process | mRNA processing, mRNA splicing |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Names & Taxonomyi
Protein namesi | Recommended name: Heterogeneous nuclear ribonucleoprotein FShort name: hnRNP F Cleaved into the following chain: |
Gene namesi | Name:Hnrnpf Synonyms:Hnrpf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2138741, Hnrnpf |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- catalytic step 2 spliceosome Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000253053 | 1 – 415 | Heterogeneous nuclear ribonucleoprotein FAdd BLAST | 415 | |
Initiator methioninei | Removed; alternateBy similarity | |||
ChainiPRO_0000367116 | 2 – 415 | Heterogeneous nuclear ribonucleoprotein F, N-terminally processedAdd BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 2 | N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processedBy similarity | 1 | |
Cross-linki | 72 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 87 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 104 | PhosphoserineBy similarity | 1 | |
Modified residuei | 107 | PhosphoserineCombined sources | 1 | |
Modified residuei | 161 | PhosphoserineBy similarity | 1 | |
Cross-linki | 167 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 185 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 187 | PhosphoserineCombined sources | 1 | |
Modified residuei | 193 | PhosphoserineBy similarity | 1 | |
Modified residuei | 195 | PhosphoserineBy similarity | 1 | |
Modified residuei | 200 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 200 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 215 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 224 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 224 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Modified residuei | 265 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Sumoylated.By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9Z2X1 |
jPOSTi | Q9Z2X1 |
PaxDbi | Q9Z2X1 |
PeptideAtlasi | Q9Z2X1 |
PRIDEi | Q9Z2X1 |
ProteomicsDBi | 269618 [Q9Z2X1-1] 269619 [Q9Z2X1-2] |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z2X1 |
PTM databases
iPTMneti | Q9Z2X1 |
PhosphoSitePlusi | Q9Z2X1 |
SwissPalmi | Q9Z2X1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000042079, Expressed in thymus and 59 other tissues |
ExpressionAtlasi | Q9Z2X1, baseline and differential |
Genevisiblei | Q9Z2X1, MM |
Interactioni
Subunit structurei
Identified in the spliceosome C complex.
Interacts with AGO1, AGO2, TBP and TXNL4/DIM1 (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 16 | Interaction with RNABy similarity | 1 | |
Sitei | 20 | Interaction with RNABy similarity | 1 | |
Sitei | 52 | Interaction with RNABy similarity | 1 | |
Sitei | 75 | Interaction with RNABy similarity | 1 | |
Sitei | 116 | Interaction with RNABy similarity | 1 | |
Sitei | 120 | Interaction with RNABy similarity | 1 | |
Sitei | 150 | Interaction with RNABy similarity | 1 | |
Sitei | 173 | Interaction with RNABy similarity | 1 | |
Sitei | 294 | Interaction with RNABy similarity | 1 | |
Sitei | 298 | Interaction with RNABy similarity | 1 | |
Sitei | 326 | Interaction with RNABy similarity | 1 | |
Sitei | 349 | Interaction with RNABy similarity | 1 |
GO - Molecular functioni
- TBP-class protein binding Source: MGI
- transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 221126, 95 interactors |
CORUMi | Q9Z2X1 |
IntActi | Q9Z2X1, 36 interactors |
MINTi | Q9Z2X1 |
STRINGi | 10090.ENSMUSP00000130023 |
Miscellaneous databases
RNActi | Q9Z2X1, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9Z2X1 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9Z2X1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 11 – 90 | RRM 1PROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 111 – 188 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 289 – 366 | RRM 3PROSITE-ProRule annotationAdd BLAST | 78 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 86 | Interaction with RNABy similarity | 6 | |
Regioni | 179 – 184 | Interaction with RNABy similarity | 6 | |
Regioni | 355 – 360 | Interaction with RNABy similarity | 6 |
Domaini
The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.By similarity
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4211, Eukaryota |
GeneTreei | ENSGT00940000157838 |
HOGENOMi | CLU_032003_1_0_1 |
InParanoidi | Q9Z2X1 |
OMAi | CYGASYS |
OrthoDBi | 603679at2759 |
PhylomeDBi | Q9Z2X1 |
TreeFami | TF316157 |
Family and domain databases
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR012996, Znf_CHHC |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits PF08080, zf-RNPHF, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 5 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9Z2X1-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMLGPEGGEG YVVKLRGLPW SCSIEDVQNF LSDCTIHDGV AGVHFIYTRE
60 70 80 90 100
GRQSGEAFVE LESEDDVKLA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
110 120 130 140 150
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
160 170 180 190 200
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
210 220 230 240 250
FMSVQRPGPY DRPGTARRYI GIVKQAGLDR MRSGAYSAGY GGYEEYSGLS
260 270 280 290 300
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
360 370 380 390 400
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
410
YGAGYSGQNS MGGYD
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketJ3QM80 | J3QM80_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpf | 232 | Annotation score: | ||
J3QMT0 | J3QMT0_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpf | 208 | Annotation score: | ||
J3QP45 | J3QP45_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpf | 70 | Annotation score: | ||
J3QMV8 | J3QMV8_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpf | 31 | Annotation score: | ||
J3QNH2 | J3QNH2_MOUSE | Heterogeneous nuclear ribonucleopro... | Hnrnpf | 14 | Annotation score: |
Sequence cautioni
The sequence AAH27003 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAH29764 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence BAC36361 differs from that shown. Reason: Erroneous termination. Extended C-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 272 | Y → C in AAH27003 (PubMed:15489334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_021004 | 1 – 22 | MMLGP…LPWSC → MW in isoform 2. 1 PublicationAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK076478 mRNA Translation: BAC36361.1 Sequence problems. BC018185 mRNA Translation: AAH18185.1 BC025481 mRNA Translation: AAH25481.1 BC027003 mRNA Translation: AAH27003.1 Different initiation. BC029163 mRNA Translation: AAH29163.1 BC029764 mRNA Translation: AAH29764.1 Different initiation. BC033483 mRNA Translation: AAH33483.1 BC089313 mRNA Translation: AAH89313.1 |
CCDSi | CCDS20467.1 [Q9Z2X1-1] |
RefSeqi | NP_001159899.1, NM_001166427.1 [Q9Z2X1-1] NP_001159900.1, NM_001166428.1 [Q9Z2X1-1] NP_001159901.1, NM_001166429.1 [Q9Z2X1-1] NP_001159902.1, NM_001166430.1 [Q9Z2X1-1] NP_001159903.1, NM_001166431.1 [Q9Z2X1-1] NP_001159904.1, NM_001166432.1 [Q9Z2X1-1] NP_598595.1, NM_133834.2 [Q9Z2X1-1] XP_006506892.1, XM_006506829.3 [Q9Z2X1-1] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK076478 mRNA Translation: BAC36361.1 Sequence problems. BC018185 mRNA Translation: AAH18185.1 BC025481 mRNA Translation: AAH25481.1 BC027003 mRNA Translation: AAH27003.1 Different initiation. BC029163 mRNA Translation: AAH29163.1 BC029764 mRNA Translation: AAH29764.1 Different initiation. BC033483 mRNA Translation: AAH33483.1 BC089313 mRNA Translation: AAH89313.1 |
CCDSi | CCDS20467.1 [Q9Z2X1-1] |
RefSeqi | NP_001159899.1, NM_001166427.1 [Q9Z2X1-1] NP_001159900.1, NM_001166428.1 [Q9Z2X1-1] NP_001159901.1, NM_001166429.1 [Q9Z2X1-1] NP_001159902.1, NM_001166430.1 [Q9Z2X1-1] NP_001159903.1, NM_001166431.1 [Q9Z2X1-1] NP_001159904.1, NM_001166432.1 [Q9Z2X1-1] NP_598595.1, NM_133834.2 [Q9Z2X1-1] XP_006506892.1, XM_006506829.3 [Q9Z2X1-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2DB1 | NMR | - | A | 1-105 | [»] | |
SMRi | Q9Z2X1 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 221126, 95 interactors |
CORUMi | Q9Z2X1 |
IntActi | Q9Z2X1, 36 interactors |
MINTi | Q9Z2X1 |
STRINGi | 10090.ENSMUSP00000130023 |
PTM databases
iPTMneti | Q9Z2X1 |
PhosphoSitePlusi | Q9Z2X1 |
SwissPalmi | Q9Z2X1 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9Z2X1 |
Proteomic databases
EPDi | Q9Z2X1 |
jPOSTi | Q9Z2X1 |
PaxDbi | Q9Z2X1 |
PeptideAtlasi | Q9Z2X1 |
PRIDEi | Q9Z2X1 |
ProteomicsDBi | 269618 [Q9Z2X1-1] 269619 [Q9Z2X1-2] |
Protocols and materials databases
Antibodypediai | 13432, 339 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 3185 |
MGIi | MGI:2138741, Hnrnpf |
Phylogenomic databases
eggNOGi | KOG4211, Eukaryota |
GeneTreei | ENSGT00940000157838 |
HOGENOMi | CLU_032003_1_0_1 |
InParanoidi | Q9Z2X1 |
OMAi | CYGASYS |
OrthoDBi | 603679at2759 |
PhylomeDBi | Q9Z2X1 |
TreeFami | TF316157 |
Enzyme and pathway databases
Reactomei | R-MMU-6803529, FGFR2 alternative splicing R-MMU-72163, mRNA Splicing - Major Pathway R-MMU-72203, Processing of Capped Intron-Containing Pre-mRNA |
Miscellaneous databases
BioGRID-ORCSi | 98758, 14 hits in 53 CRISPR screens |
ChiTaRSi | Hnrnpf, mouse |
EvolutionaryTracei | Q9Z2X1 |
PROi | PR:Q9Z2X1 |
RNActi | Q9Z2X1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042079, Expressed in thymus and 59 other tissues |
ExpressionAtlasi | Q9Z2X1, baseline and differential |
Genevisiblei | Q9Z2X1, MM |
Family and domain databases
Gene3Di | 3.30.70.330, 3 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR012996, Znf_CHHC |
Pfami | View protein in Pfam PF00076, RRM_1, 2 hits PF08080, zf-RNPHF, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 3 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits |
PROSITEi | View protein in PROSITE PS50102, RRM, 3 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HNRPF_MOUSE | |
Accessioni | Q9Z2X1Primary (citable) accession number: Q9Z2X1 Secondary accession number(s): Q5FWK2 Q8R0E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 17, 2006 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 183 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references